138 research outputs found
Efficacy of fipronil/(S)-methoprene/eprinomectin/praziquantel (Broadline®) against Thelazia callipaeda in naturally infected cats
Bronchopulmonary Nematodes in Alpine Ibex: Shedding of First Stage Larvae Analyzed at the Individual Host Level
Wild boar ecology: a review of wild boar ecological and demographic parameters by bioregion all over Europe
Prognostic value of immunoglobulin G (IgG) patterns by western blotting immunodetection in treated dogs previously infected with Leishmania infantum
Reduced efficacy of fenbendazole and pyrantel pamoate treatments against intestinal nematodes of stud and performance horses
Development and Evaluation of qPCR Detection Method and Zn-MgO/Alginate Active Packaging for Controlling Listeria monocytogenes Contamination in Cold-Smoked Salmon
To answer to food industry requests to monitor the presence of L. monocytogenes in
cold-smoked salmon samples and to extend their shelf-life, a qPCR protocol for the detection of
L. monocytogenes, and an antibacterial active packaging reinforced with zinc magnesium oxide
nanoparticles (Zn-MgO NPs) were developed. The qPCR allowed the sensitive and easy detection
of L. monocytogenes in naturally contaminated samples, with specificity in full agreement with the
standard methods. The halo diusion study indicated a high antibacterial eciency of 1 mg/mL
Zn-MgO NPs against L. monocytogenes, while the flow cytometry showed only moderate cytotoxicity
of the nanoparticles towards mammalian cells at a concentration above 1 mg/mL. Thus, the novel
active packaging was developed by using 1 mg/mL of Zn-MgO NPs to reinforce the alginate film.
Cold-smoked salmon samples inoculated with L. monocytogenes and air-packed with the Zn-MgO
NPs-alginate nanobiocomposite film showed no bacterial proliferation at 4 C during 4 days. In the
same condition, L. monocytogenes growth in control contaminated samples packed with alginate
film alone. Our results suggest that Zn-MgO nanoparticles can extend the shelf-life of cold-smoked salmon samples
Molecular differentiation of cattle Sarcocystis spp. by multiplex PCR targeting 18S and COI genes following identification of Sarcocystis hominis in human stool samples
Integrating environmental, entomological, animal, and human data to model the Leishmania infantum transmission risk in a newly endemic area in Northern Italy
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