Monitoring regional development based on "green" indicators

The article presents the results of a study devoted to solving actual problems of implementing the concept of "green" development both at national and regional levels. The indicators of "green" development developed by international organizations are considered as a methodological basis for the creation of systems of monitoring and analyzing the processes of economy "greening", preventing and reducing current negative impact on the environment, increasing the efficiency of resource use in the context of carbon regulation. Assessment of the dynamics of the development of the Voronezh region has been carried out on the basis of selected indicators, and it has been concluded that the regional policy takes into account the necessary prerequisites for "green" growth, which determine the need to increase environmental, economic and social efficiency of activities, increasing the level of waste processing and reduce their producing.peer-reviewe

НЕВРОЛОГИЧЕСКИЕ АСПЕКТЫ У ДЕТЕЙ С ПЕРИНАТАЛЬНОЙ ВИЧ-ИНФЕКЦИЕЙ

In the given research results of inspection of children with a HIV-infection are analyzed at perinatal ways. 550 children from a birth till 10 years are surveyed. The neurological status of patients, the data immunological and virologic inspection of plasma and cerebrospinal fluid is studied. It is spent neuroimaging and psychometric testing. It is shown that defeat of nervous system is HIV-infection display at children’s age. A HIV-induced defeat of the central and peripheral nervous system is presented, first of all, to specific HIV-encephalitis with sharp and sub acute a current. Are revealed clinical and immunological correlations at various stages of infectious process, influence antiretroviral therapies on dynamics of neurological infringements is analyzed.В данном исследовании проанализированы результаты обследования детей с ВИЧ-инфекцией при перинатальном пути инфицирования. Обследовано 550 детей от рождения до 10 лет. Изучен неврологический статус пациентов, данные иммунологического и вирусологического обследования плазмы и ликвора. Проведена нейровизуализация и психометрическое тестирование. Показано, что поражение нервной системы является патогномоничным проявлением ВИЧ-инфекции в детском возрасте. ВИЧ-индуцированное поражение центральной и периферической нервной системы представлено, прежде всего, специфическим ВИЧ-энцефалитом с острым и подострым течением. Выявлены клинико-иммунологические корреляции на различных стадиях инфекционного процесса, проанализировано влияние антиретровирусной терапии на динамику неврологических нарушений

DANSSino: a pilot version of the DANSS neutrino detector

DANSSino is a reduced pilot version of a solid-state detector of reactor antineutrinos (to be created within the DANSS project and installed under the industrial 3 GW(th) reactor of the Kalinin Nuclear Power Plant -- KNPP). Numerous tests performed at a distance of 11 m from the reactor core demonstrate operability of the chosen design and reveal the main sources of the background. In spite of its small size (20x20x100 ccm), the pilot detector turned out to be quite sensitive to reactor antineutrinos, detecting about 70 IBD events per day with the signal-to-background ratio about unity.Comment: 16 pages, 11 figures, 3 tables. arXiv admin note: substantial text overlap with arXiv:1304.369

To the problem of essential early diagnostics of funicular myelosis

Purpose: to assess indices of peripheral blood and marrow of patients with funicular myelosis in comparison with anamnesis data in order to establish the reason of mielinopathy development to prescribe early pathogenic therapy. Material and Methods: 25 patients aged 42-79, with verified funicular myelosis, having various possible reasons of myelinopathy development in anamnesis. The received results: the assessment of indices of peripheral blood and marrow revealed changes characteristic of В12 deficiency anaemia. Early diagnostics of В-12 deficiency anaemia and well-timed beginning of treatment have been shown to result in complete regression of clinical symptoms of funicular myelosis in all patients, whereas in cases with prolongation of the disease for more than 6 months even adequate therapy resulted only in partial regression of neurologic symptomatology. Summary: presence of clinical sings of funicular myelosis, even at absence of changes of laboratory blood indices, requires more profound examination and more scrupulous anamnesis taking

Search for sterile neutrinos at the DANSS experiment

DANSS is a highly segmented 1~m${}^3$ plastic scintillator detector. Its 2500 one meter long scintillator strips have a Gd-loaded reflective cover. The DANSS detector is placed under an industrial 3.1~$\mathrm{GW_{th}}$ reactor of the Kalinin Nuclear Power Plant 350~km NW from Moscow. The distance to the core is varied on-line from 10.7~m to 12.7~m. The reactor building provides about 50~m water-equivalent shielding against the cosmic background. DANSS detects almost 5000 $\widetilde\nu_e$ per day at the closest position with the cosmic background less than 3$\%$. The inverse beta decay process is used to detect $\widetilde\nu_e$. Sterile neutrinos are searched for assuming the $4\nu$ model (3 active and 1 sterile $\nu$). The exclusion area in the $\Delta m_{14}^2,\sin^22\theta_{14}$ plane is obtained using a ratio of positron energy spectra collected at different distances. Therefore results do not depend on the shape and normalization of the reactor $\widetilde\nu_e$ spectrum, as well as on the detector efficiency. Results are based on 966 thousand antineutrino events collected at 3 distances from the reactor core. The excluded area covers a wide range of the sterile neutrino parameters up to $\sin^22\theta_{14}<0.01$ in the most sensitive region.Comment: 10 pages, 13 figures, version accepted for publicatio

Difficulties in diagnosing atypical hemolytic uremic syndrome

The paper presents the case of clinical observation of a patient with atypical hemolytic-uremic syndrome (aHUS). aHUS is a disease characterized by an unfavorable prognosis (severe or catastrophic course with rapid development of terminal renal or multi-organ failure). The aim of the study is to evaluate the approaches to differential diagnosis of aHUS in clinical practice.Material and methods. The study was conducted on the basis of the Nephrology Department of Kemerovo Regional Clinical Hospital n.a. S.V Belyaev. The clinical observation of patient D., aged 26 years old, is discussed.Results and discussion. Diagnosing aHUS requires: 1) diagnosing thrombotic microangiopathy (TMA: thrombocytopenia or decrease in platelet count by more than 25 % of original, visceral damage (kidneys, CNS, gastrointestinal tract, heart, lungs)); 2) ruling out HUS associated with Shiga toxin-producing Escherichia coli (STEC-HUS; negative for Shiga-toxin in blood and stool), thrombotic thrombocytopenic purpura (TTP), systemic connective tissue disease, catastrophic antiphospholipid syndrome, HIV infection; 3) assessing the activity of ADAMTS13 (decrease confirms the aHUS diagnosis); 4) proving normal content of complement components C3 and C4 as an additional argument in favor of aHUS diagnosis. At the first stage, the patient was diagnosed with TMA (platelet content 37 x 109/l, hemoglobin content 59 g/l), LDH up to 824 E/l), liver damage (AST, ALT and LDH activity 55, 60 and 824 U/l, respectively), kidney damage (acute renal damage), lungs, heart, and brain damage. At the second stage the following diagnoses were ruled out: STEC-HUS (Shiga toxin in blood and stool was not detected), TTP (ADAMTS13 activity level was 66 %, whereas reference values are 93-113 %, in TTP - below 5-10 %); systemic connective tissue diseases catastrophic antiphospholipid syndrome, HIV infection sepsis. Normal values of C3 (0.9 g/l) and C4 (0.23 g/l) complement components did not rule out the diagnosis of aHUS

Nomenclatural standards and genetic passports of potato cultivars bred by the Tatar Research Institute of Agriculture «Kazan Scientific Center of the Russian Academy of Sciences»

Breeding of potato cultivars adapted to the climatic conditions of the Middle Volga region and resistant to various harmful organisms has always been an urgent task for breeders of the Tatarstan Republic. In the present paper, the potato cultivars bred at the Tatar Research Institute of Agriculture – Kazan Scientific Center of the Russian Academy of Sciences, were taken as an example for demonstrating the results of application and elaboration of methodological approaches that are currently developed at the N.I. Vavilov Institute of Plant Genetic Resources (VIR) for the preparing of nomenclatural standards and their genotyping. At the initiative of the VIR researchers in collaboration with the authors of potato cultivars E.A. Gimaeva and Z. Stashevski plant material was collected and nomenclatural standards for potato cultivars bred at the Kazan Scientific Center of the Russian Academy of Sciences were prepared. Four nomenclatural standards for Tatarstan potato cultivars (‘Zumba’, ‘Kortni’, ‘Reggi’, ‘Samba’) were prepared in accordance with the provisions of the International Code of Nomenclature For Cultivated Plants, registered in the VIR Herbarium Database, and transferred to the VIR herbarium type collection. Besides, voucher specimens of cv. ‘Tango’ and breeding clone ‘Sal’sa’, which is undergoing the State Variety Test at present, were produced. Genetic passports of Tatarstan varieties (‘Zumba’, ‘Kortni’, ‘Reggi’, ‘Samba’) and one breeding clone (‘Sal’sa’)* were developed using DNA samples from plant material transferred by the authors of the cultivars to the VIR herbarium. The genetic passports include information of the polymorphism of eight chromosome-specific microsatellites, data on the presence/ absence of the diagnostic fragments of 15 markers of the 11 R-genes conferring resistance to various harmful organisms and the information about cytoplasm types. A comparison of the data from genetic passports developed using DNA samples from nomenclatural standard specimens with the results of genotyping of 22 samples of Tatarstan cultivars obtained from different sources (e.g., samples undergoing ecogeographic tests within the framework of the Comprehensive Research Plan of the subprogram “Development of potato breeding and seed production in the Russian Federation”; as well as accessions from the VIR in vitro collection) made it possible to assess the authenticity and uniformity of the studied plant material

Baseline Demographics, Comorbidities, Treatment Patterns and Burden of Atopic Dermatitis in Adults and Adolescents from the GLOBOSTAD Long-Term Observational Study

Introduction - Insights into real-world treatment of atopic dermatitis (AD) are relevant to clinical decision making. The aim of this analysis was to characterize patients who receive dupilumab for AD in a real-world setting. Methods - The GLOBOSTAD registry is an ongoing, longitudinal, prospective, observational study of patients with AD who receive dupilumab according to country-specific prescribing information. We report baseline characteristics, comorbidities and treatment patterns for patients enrolled from July 11, 2019 to March 31, 2022. Analyses are descriptive; no formal statistical comparisons were performed. Results - Nine hundred fifty-two adults and adolescents were enrolled in GLOBOSTAD. Patients had a high disease burden before starting dupilumab: (mean [standard deviation]) percent body surface area affected (44.8 [24.42]), Eczema Area and Severity Index total score (24.8 [12.95]), SCORing Atopic Dermatitis total score (60.5 [16.34]), Patient-Oriented Eczema Measure total score (19.7 [6.37]) and Dermatology Life Quality Index total score (13.7 [7.02]). Overall, 741 (77.8%) patients reported ≥ 1 type 2 inflammatory comorbidities, most frequently allergic rhinitis (492 [51.7%]), asthma (323 [33.9%]), food allergy (294 [30.9%]) or another allergy (274 [28.8%]). In the previous 12 months, 310 (32.6%) patients had received systemic non-steroidal immunosuppressants and 169 (17.8%) systemic corticosteroids; 449 (47.2%) had received topical corticosteroids, most commonly potent topical corticosteroids; 141 (14.8%) had received topical calcineurin inhibitors and 32 (3.4%) ultraviolet therapy. Most (713 [74.9%]) patients started dupilumab because of prior treatment failure. Conclusion - Patients enrolled in GLOBOSTAD demonstrated considerable multidimensional burden of disease across AD signs, symptoms and quality of life despite previous use of systemic and non-systemic AD treatments

Идентификация видов рыбы с помощью технологии секвенирования нового поколения (NGS)

The laws relating to fish and fishery product labeling that require indication of the information about fish species exist in many world countries. These rules are conditioned by a significant growth in the number of the economic fraud cases in the field of production and trade of fishery products. The widespread ways of fraud are replacement and mislabeling of a product as confirmed by many studies. Analysis of scientific works shows that mislabeling in fishery product manufacture occurs in 30–70% of cases in different countries. The existing legislation about food traceability is insufficient for their prevention, which suggests a necessity of taking strict control measures ensuring effective species identification of fish and fishery products. At present, various laboratory tests are used for their species identification. They are based, mainly, on analysis of unique DNA profiles found in different species. In this work, we present the method for detection of fish species using next generation sequencing (NGS). NGS is an advanced technology in the field of quality control of fishery products, especially for fish species identification in multicomponent products, which contain DNA fragments of other species besides the target DNA. NGS was carried out on the platform Ion Torrent Ion GeneStudio S5 System. Twenty samples were analyzed: 17 commercial samples and three prepared experimental samples consisted of the mixture of two and more species. The universal primers, which were able to amplify the fragment 16S rRNA of the commercial fish species, were selected and prepared. In general, DNA of 11 families, 15 genera and 16 species was identified in the course of the analysis. The obtained result of NGS of 17 commercial samples confirmed the results of identification by other molecular diagnostic methods. Mislabeling was revealed in four samples. In three samples, all fish species present in the composition were identified. Possible reasons for fish replacement were assessed.Законы о маркировке рыбы и рыбной продукции, требующие указывать информацию о видовой принадлежности рыбы, есть во многих странах мира. Данные правила обусловлены высоким ростом количества случаев экономического мошенничества в области производства и оборота рыбных продуктов. Распространенными способами мошенничества являются подмена и  неправильная маркировка продукта, что подтверждается многочисленными исследованиями. Анализ научных работ показал, что применение неправильной маркировки при производстве рыбных продуктов в разных странах встречается в 30–70% случаев. Для их предотвращения имеющегося законодательства о прослеживаемости пищевых продуктов недостаточно, что указывает на необходимость принятия строгих мер контроля, обеспечивающих эффективную видовую идентификацию рыбы и рыбопродуктов. В настоящее время для идентификации их видовой принадлежности используют различные лабораторные тесты, главным образом основанные на анализе уникальных профилей ДНК, которые обнаружены у различных видов. В данной работе представлен метод определения видовой принадлежности рыб с использованием секвенирования нового поколения (NGS). Технология секвенирования NGS является передовой в области контроля качества рыбной продукции, особенно для идентификации видов рыб в многокомпонентной продукции, в которой помимо целевой ДНК присутствуют и фрагменты ДНК других видов. Секвенирование NGS проводили на платформе Ion Torrent Ion GeneStudio S5 System, были проанализированы двадцать образцов, 17 коммерческих образцов и 3 приготовленных опытных образца, состоящих их смеси двух и более видов. Были подобраны и подготовлены универсальные праймеры, способные амплифицировать фрагмент 16S rRNA промысловых видов рыб. В целом в ходе анализа была идентифицирована ДНК 11 семейств, 15 родов и 16 видов. Полученный результат секвенирования NGS17 коммерческих образцов подтверждал результаты идентификации другими молекулярно-диагностическими методами, была выявлена неправильная маркировка в 4 образцах. В 3 опытных образцах были идентифицированы все виды рыб, входящих в их состав. Была произведена оценка возможных причин замены рыбы