Study of Biological Properties of Effective Pesticides Destructor Pseudomonas Putida P2

A promising approach for remediation of contaminated soils is the use of biological products based on microorganisms-destructors. Of particular interest are the strains that can decompose several types of pollutants. Potential strains-destructors should be not only effective but also adapted for certain soil and climatic conditions. In addition, when introducing a significant amount of biological agents into the ecosystem, the lack of toxicological and pathogenic effects on plants, animals, and humans is particularly important. The object of the study is the destructor strain of sim-triazine herbicide of prometrin Pseudomonas putida P2. The study explores the ability of strain to use various pesticides as the only carbon source, the effect of bacterial culture on seed germination, biotesting of strain on toxicity using earthworms. Besides, the study presents the phytopathogenic factors and enzymes causing bacteria invasiveness into animals and humans. The determination of dehydrogenase activity of prometrin destructor strain shows that this strain can also use active ingredients of pesticide malathion, HCH, and 4,4-DDT as only sole carbon source. It is also established that the studied strain Pseudomonas putida P2 increases the germination of seeds of the variety Saratovskaya 70 and carrots of the variety Samson and does not possess pathogenicity factors. The strain biotesting on the earthworms revealed no acute toxicity of the culture. The analysis of data obtained from the model pollution shows that the introduction of the destructor of Prometrium Pseudomonas putida P2 into the soil can create favorable conditions for vital activity and reproduction of earthworms

Chicken manure as an organic fertilizer: composting technologies and impact on soil properties (a review)

Chicken manure and composts derived from it are valuable organic fertilizers that have a positive impact on soil fertility and significantly enhance crop yields. However, the application of fresh manure can lead to excessive concentrations of nitrogen and other nutrients in the soil, as well as contamination of the soil with microorganisms that are pathogenic to humans and animals. Therefore, it is recommended to compost chicken manure prior to its use in agriculture. This review examines the composition and properties of chicken manure, the techniques involved in its composting, and its influence on the physical, chemical, and biological properties of the soil. The utilization of composted manure results in increased levels of organic carbon (on average by 1–1.5%), nitrogen (by 0.1–0.2%), and other biophilic elements. Moreover, it improves various physical properties of the soil, such as porosity, aeration, and density. Levels of pH increase (by 0.2–0.4), as well as electrical conductivity (by 2–2.5 times), and the quantity of water-resistant aggregates (by 5–10%). The application of these fertilizers also promotes biological activity, microbial diversity, microbial biomass carbon (by 25–75%), and soil enzymatic activity (phosphatase and catalase increase by 5–7 times, and urease by 3–5 times). When chicken manure is incorporated into the soil, a significant (2–4 times) increase in the population of bacteria from the phyla Firmicutes, Bacteroidetes, and Actinobacteria is observed. The necessity of investigating the effects of compost derived from chicken manure on the chemical composition and storage of soil organic matter, pore space, and the ecotrophic structure of the soil microbiome is also discussed

Comparative evaluation of the influence of no-till and traditional treatment on the biological activity of agricultural chernozem of the Stavropol region

Using the method of inoculation on elective nutrient media, the abundance and activity of taxonomic (bacteria, actinomycetes, micromycetes) and functional (nitrogen fixers, ammonifiers, denitrifiers of amylolytic, cellulolytic) groups of microorganisms in agrochernozems of the Stavropol region under the influence of various tillage systems – direct seeding (no-till) and moldboard plowing with the turnover of soil horizon (traditional treatment). Analyzes were carried out for such crops as winter wheat, corn, sunflower and soybean with/without application of mineral fertilizers. Traditional tillage for the majority of crops leads to increased activity of aerobic ammonifiers, cellulolytics, denitrifiers, actinomycetes and micromycetes. No-till technology, in its turn, increases the intensity of anaerobic cellulolytics and nitrogen fixers, aerobic diazotrophs and amylolytics activity. The most responsive crop, under which the biological activity of almost all groups of microorganisms increased in the no-till variant, was corn, while winter wheat contributed to the increase in the number of microorganisms in the moldboard plowing variant. The use of mineral fertilizers provoked the growth of soil biological activity under sunflower sown directly in the soil, while conventional tillage resulted in higher soil biological activity without application of mineral fertilizers

Creation of multimedia presentations — effective form of educational technologies in independent work of students

The article is devoted to the such a special kind of student's activity as PowerРoint multimedia presentations. The authors analysis the role of this kind of activity at the medical students' education and it's contribution to the training a lot of professional and personal's features.В статье рассматривается создание мультимедийных презентаций в Powerpoint в качестве эффективной формы организации самостоятельной работы студента. Авторы анализируют роль этого вида деятельности в образовании студентов лечебно-профилактического факультета и его вклад в развитие многих профессиональных и личностных качеств

Криомассаж грудной клетки и сильвинитовая спелеотерапия в комплексной медицинской реабилитации больных бронхиальной астмой

Summary. The study was conducted to evaluate therapeutic and preventive effect of chest cryomassage and sylvinite speleotherapy in addition to the standard drug therapy in patients with mild to moderate persistent partially controlled bronchial asthma. Effects of a comprehensive rehabilitation programme were confirmed by a distinct reduction in eosinophilic and neutrophilic inflammation, significant improvements in systemic immunity, lung function, cardiorespitatory hemodynamics, exercise tolerance, and psychological adaptation.Резюме. Изучено лечебно-профилактическое действие криомассажа грудной клетки и сильвинитовой спелеотерапии на фоне стандартного медикаментозного лечения у больных легкой и среднетяжелой персистирующей бронхиальной астмой с частично контролируемым течением. Отчетливый регресс эозинофильного и нейтрофильного воспаления, выраженное улучшение показателей системного иммунитета, внешнего дыхания, легочной кардиогемодинамики, физической толерантности, психологической адаптации позволил констатировать преимущество комплексного применения реабилитационных методов

N-ARYLBENZAMIDINES HYDROCHLORIDES. SYNTHESIS AND STRUCTURE

The reaction of obtaining N-arylbenzamidines hydrochlorides was studied. The structure of synthesized compounds was proved with the help of modern physicochemical methods of analysis: 1Н-, 13С-spectroscopy, X-ray diffraction analysis (RSA)

Study of Biological Properties of Effective Pesticides Destructor Pseudomonas Putida P2

A promising approach for remediation of contaminated soils is the use of biological products based on microorganisms-destructors. Of particular interest are the strains that can decompose several types of pollutants. Potential strains-destructors should be not only effective but also adapted for certain soil and climatic conditions. In addition, when introducing a significant amount of biological agents into the ecosystem, the lack of toxicological and pathogenic effects on plants, animals, and humans is particularly important. The object of the study is the destructor strain of sim-triazine herbicide of prometrin Pseudomonas putida P2. The study explores the ability of strain to use various pesticides as the only carbon source, the effect of bacterial culture on seed germination, biotesting of strain on toxicity using earthworms. Besides, the study presents the phytopathogenic factors and enzymes causing bacteria invasiveness into animals and humans. The determination of dehydrogenase activity of prometrin destructor strain shows that this strain can also use active ingredients of pesticide malathion, HCH, and 4,4-DDT as only sole carbon source. It is also established that the studied strain Pseudomonas putida P2 increases the germination of seeds of the variety Saratovskaya 70 and carrots of the variety Samson and does not possess pathogenicity factors. The strain biotesting on the earthworms revealed no acute toxicity of the culture. The analysis of data obtained from the model pollution shows that the introduction of the destructor of Prometrium Pseudomonas putida P2 into the soil can create favorable conditions for vital activity and reproduction of earthworms

PATIENT WITH PRIMARY PULMONARY HYPERTENSION – WHO HE IS AND HOW TO HELP HIM?

Differential diagnostic search in patients with pulmonary hypertension is always difficult clinical problem. This article describes the clinical case of treating patient with high risk primary pulmonary hypertension.</p