1 research outputs found
Π‘ΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΎΡΠΎΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΈΡ ΡΠ²ΠΎΠΉΡΡΠ² ΠΌΠ΅ΡΠΈΠ»Π΅Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΈΠ½Π΅Π³ΠΎ Π² Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ ΡΡΠ΅Π΄Π°Ρ
A spectroscopic study of the photophysical properties of methylene blue (MB) in aqueous solutions was carried out. Absorption and fluorescence spectra as well as fluorescence lifetime were recorded. The concentration dependence of the intensity and shape of the spectra allowed establishing the ranges of MB concentrations for in vitro and in vivo studies at which aggregation is not observed (up to 0.01 mM, which corresponds to 3.2 mg/kg). Studies of photodegradation in biological media showed that photobleaching of more than 80% in plasma and culture media is observed already at a dose of 5 J/cm2 , while in water at this concentration and dose photobleaching is not yet observed, and at a dose of 50 J/cm2 Β photobleaching of MB is about 30%. It was found that in media containing proteins and having an alkaline pH, photobleaching occurs significantly faster than in neutral aqueous media. The ionic strength of the solution has no effect on the photobleaching rate. Such photobleaching is caused by the photodegradation of MB rather than the transition to the leucoform.The efficiency of singlet oxygen generation and photodynamic activity were evaluated in vitro. In the investigated range of MB concentrations, the efficiency of singlet oxygen generation is rather low, because positively charged MB binds to negatively charged cell membranes, which leads to a change in the type of photodynamic reaction. The emergence of other reactive oxygen species (ROS), different from singlet oxygen, in cells has been demonstrated. The generation of ROS and the low quantum yield of singlet oxygen generation indicate the tendency of MB to provide the type I photosensitization mechanism (electron transfer with the formation of semi-reduced and semi-oxidized MB+ radicals) rather than to the type II mechanism (energy transfer to oxygen with the formation of singlet oxygen) in biological media and in vivo.ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΎΡΠΎΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ²ΠΎΠΉΡΡΠ² ΠΌΠ΅ΡΠΈΠ»Π΅Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΈΠ½Π΅Π³ΠΎ (ΠΠ‘) Π² Π²ΠΎΠ΄Π½ΡΡ
ΡΠ°ΡΡΠ²ΠΎΡΠ°Ρ
ΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΆΠΈΠ΄ΠΊΠΎΡΡΡΡ
. ΠΠ°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½Ρ ΡΠΏΠ΅ΠΊΡΡΡ ΠΏΠΎΠ³Π»ΠΎΡΠ΅Π½ΠΈΡ ΠΈ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠΈΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ Π²ΡΠ΅ΠΌΠ΅Π½Π° ΠΆΠΈΠ·Π½ΠΈ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠΈΠΈ. ΠΠΎ Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈ ΡΠΎΡΠΌΡ ΡΠΏΠ΅ΠΊΡΡΠΎΠ² ΠΎΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΡΠ΄Π°Π»ΠΎΡΡ ΡΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Ρ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΉ ΠΠ‘ Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ in vitro ΠΈ in vivo ΠΏΡΠΈ ΠΊΠΎΡΠΎΡΡΡ
Π½Π΅ Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ Π°Π³ΡΠ΅Π³Π°ΡΠΈΡ (Π΄ΠΎ 0,01 ΠΌΠ, ΡΡΠΎ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΠ΅Ρ 3,2 ΠΌΠ³/ΠΊΠ³). ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ ΡΠΎΡΠΎΠΎΠ±Π΅ΡΡΠ²Π΅ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΠΠ‘ ΠΏΠΎΠ΄ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ Π»Π°Π·Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΡ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠΎΡΠΎΠ΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΠΈ Π² Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΡΠ΅Π΄Π°Ρ
ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ, ΡΡΠΎ ΡΠΎΡΠΎΠΎΠ±Π΅ΡΡΠ²Π΅ΡΠΈΠ²Π°Π½ΠΈΠ΅ Π±ΠΎΠ»Π΅Π΅ ΡΠ΅ΠΌ Π½Π° 80% Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΈ ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΠ΅Π΄Π΅ Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ ΡΠΆΠ΅ ΠΏΡΠΈ Π΄ΠΎΠ·Π΅ 5 ΠΠΆ/ΡΠΌ2 , Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ Π² Π²ΠΎΠ΄Π΅ ΠΏΡΠΈ ΡΠ°ΠΊΠΎΠΉ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΏΡΠΈ Π΄ΠΎΠ·Π΅ 5 ΠΠΆ/ΡΠΌ2 Β ΡΠΎΡΠΎΠΎΠ±Π΅ΡΡΠ²Π΅ΡΠΈΠ²Π°Π½ΠΈΡ Π΅ΡΠ΅ Π½Π΅ Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ, Π° ΠΏΡΠΈ Π΄ΠΎΠ·Π΅ 50 ΠΠΆ/ΡΠΌ2 Β ΡΠΎΡΠΎΠΎΠ±Π΅ΡΡΠ²Π΅ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΠΠ‘ ΡΠΎΡΡΠ°Π²Π»ΡΠ΅Ρ ΠΏΠΎΡΡΠ΄ΠΊΠ° 30%. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π² ΡΡΠ΅Π΄Π°Ρ
, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ
Π±Π΅Π»ΠΊΠΈ ΠΈ ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΡ
ΡΠ΅Π»ΠΎΡΠ½ΡΠΌ ΡΠ, ΡΠΎΡΠΎΠΎΠ±Π΅ΡΡΠ²Π΅ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΠΈΡ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ Π±ΡΡΡΡΠ΅Π΅, ΡΠ΅ΠΌ Π² Π½Π΅ΠΉΡΡΠ°Π»ΡΠ½ΡΡ
Π²ΠΎΠ΄Π½ΡΡ
ΡΡΠ΅Π΄Π°Ρ
. ΠΠΎΠ½Π½Π°Ρ ΡΠΈΠ»Π° ΡΠ°ΡΡΠ²ΠΎΡΠ° Π½Π΅ ΠΎΠΊΠ°Π·ΡΠ²Π°Π΅Ρ Π²Π»ΠΈΡΠ½ΠΈΡ Π½Π° ΡΠΊΠΎΡΠΎΡΡΡ ΡΠΎΡΠΎΠΎΠ±Π΅ΡΡΠ²Π΅ΡΠΈΠ²Π°Π½ΠΈΡ. Π’Π°ΠΊΠΎΠ΅ ΡΠΎΡΠΎΠΎΠ±Π΅ΡΡΠ²Π΅ΡΠΈΠ²Π°Π½ΠΈΠ΅ Π²ΡΠ·Π²Π°Π½ΠΎ ΡΠΎΡΠΎΠ΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΠ΅ΠΉ ΠΠ‘, Π° Π½Π΅ ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄ΠΎΠΌ Π² Π»Π΅ΠΉΠΊΠΎΡΠΎΡΠΌΡ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΠΎΡΠ΅Π½ΠΊΠ° ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ ΡΠΈΠ½Π³Π»Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄Π° ΠΈ ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ in vitro. Π ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠΎΠΌ Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΉ ΠΠ‘ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ ΡΠΈΠ½Π³Π»Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄Π° Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ Π½ΠΈΠ·ΠΊΠ°Ρ, ΡΠ°ΠΊ ΠΊΠ°ΠΊ ΠΏΠΎΠ»ΠΎΠΆΠΈΡΠ΅Π»ΡΠ½ΠΎ Π·Π°ΡΡΠΆΠ΅Π½Π½ΡΠΉ ΠΠ‘ ΡΠ²ΡΠ·ΡΠ²Π°Π΅ΡΡΡ Ρ Π½Π΅Π³Π°ΡΠΈΠ²Π½ΠΎ Π·Π°ΡΡΠΆΠ΅Π½Π½ΡΠΌΠΈ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π°ΠΌΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ, ΡΡΠΎ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΈΠΏΠ° ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ. ΠΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ Π²ΠΎΠ·Π½ΠΈΠΊΠ½ΠΎΠ²Π΅Π½ΠΈΠ΅ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
Π΄ΡΡΠ³ΠΈΡ
Π°ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠΎΡΠΌ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄Π° (ΠΠ€Π), ΠΎΡΠ»ΠΈΡΠ½ΡΡ
ΠΎΡ ΡΠΈΠ½Π³Π»Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄Π°. ΠΠ΅Π½Π΅ΡΠ°ΡΠΈΡ ΠΠ€Π ΠΈ Π½Π΅Π²ΡΡΠΎΠΊΠΈΠΉ ΠΊΠ²Π°Π½ΡΠΎΠ²ΠΎΠΌ Π²ΡΡ
ΠΎΠ΄ Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ ΡΠΈΠ½Π³Π»Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄Π° ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΡΠΊΠ»ΠΎΠ½Π½ΠΎΡΡΠΈ ΠΠ‘ ΠΊ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΡ ΡΠΎΡΠΎΡΠ΅Π½ΡΠΈΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ I ΡΠΈΠΏΠ° (ΠΏΠ΅ΡΠ΅Π½ΠΎΡ ΡΠ»Π΅ΠΊΡΡΠΎΠ½Π° Ρ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΏΠΎΠ»ΡΠ²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½ΡΡ
ΠΈ ΠΏΠΎΠ»ΡΠΎΠΊΠΈΡΠ»Π΅Π½Π½ΡΡ
ΡΠ°Π΄ΠΈΠΊΠ°Π»ΠΎΠ² MB+), Π° Π½Π΅ ΠΊ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΡ II ΡΠΈΠΏΠ° (ΠΏΠ΅ΡΠ΅Π½ΠΎΡ ΡΠ½Π΅ΡΠ³ΠΈΠΈ ΠΊ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄Ρ Ρ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠΈΠ½Π³Π»Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄Π°) Π² Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΡΠ΅Π΄Π°Ρ
ΠΈ in vivo