Spiroplasma kunkelii: controle genético da resistência do milho.

Trabalho apresentado no 42º Congresso Brasileiro de Fitopatologia, 2009. Rio de Janeiro, RJ

Long-term efficacy of OROS® hydromorphone combined with pregabalin for chronic non-cancer neuropathic pain.

BACKGROUND AND OBJECTIVES: Treatment for chronic non-cancer neuropathic pain can be complicated by side effects and drug interactions. Combining opioid analgesics and calcium channel modulators may overcome these and improve efficacy. The objective of the present study was to evaluate the efficacy and safety of OROS® hydromorphone combined with pregabalin in patients with chronic non-cancer neuropathic pain. METHODS: This retrospective observational study was conducted on clinical records from patients aged ≥18 years with chronic non-cancer neuropathic [>4 on the Douleur Neuropathique en 4 questions (DN4) scale] pain of ≥6 months duration, with severe intensity [>4 on the Numerical Rating Scale (NRS); range 0-10], who attended all visits and had ≥12 months of follow-up at the Tor Vergata University Polyclinic Hospital, from November 2008 to February 2011. Patients received an oral combination of OROS® hydromorphone and pregabalin. Pain was evaluated at each visit (months 1, 3, 6, 9, and 12) using the NRS and DN4 scale; Patients' Global Impression of Change (PGIC) was administered at months 1, 6, and 12. Dosage and side effects were recorded at each visit. RESULTS: Of 1,292 patients (32 % men, mean ± SD age 67.6 ± 11.9 years), 1,126 attended all visits. Seventeen percent (n = 224) had purely neuropathic pain. Initial mean dosage was 6.06 ± 2.00 mg/day for OROS® hydromorphone, 113.02 ± 21.94 mg/day for pregabalin. Dosages increased up to month 6, and returned to near initial dosages at month 12 (range 4-120 mg/day for OROS® hydromorphone; 75-600 mg/day for pregabalin). NRS pain scores (mean ± standard deviation) were 7.25 ± 1.34 at baseline and 1.85 ± 1.36 at 12 months (p < 0.0001); DN4 scores were 6.19 ± 1.65 at baseline, reduced to 1.84 ± 1.25 at 12 months (p < 0.0001), reductions of 74.4 and 70.2 %, respectively. More than 90 % of patients had a ≥50 % score reduction on both scales after 12 months. The PGIC scale showed that >75 % of patients felt improvement at 1 month, increasing to 91 % and 93 % at 6 and 12 months. The incidence of side effects was similar between elderly (aged >65 years) and younger subjects; there were no cases of addiction. CONCLUSIONS: The OROS® hydromorphone and pregabalin combination was efficacious for chronic non-cancer neuropathic pain and well tolerated, providing significant pain reduction without the risk of addiction and with a good tolerability profile, regardless of age

Influência da temperatura nos sintomas causados pelo enfezamento pálido do milho.

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Effect of the corn stunt spiroplasma disease on maize production.

Suplemento. Edição dos Abstracts do 10º International Congress of Plant Pathology, Beijing, 2013

Effects of environmental temperature on the corn stunt spiroplasma disease.

Suplemento. Edição dos Abstracts do 10º International Congress of Plant Pathology, Beijing, 2013

Sonic hedgehog medulloblastoma cancer stem cells mirnome and transcriptome highlight novel functional networks

Molecular classification has improved the knowledge of medulloblastoma (MB), the most common malignant brain tumour in children, however current treatments cause severe side effects in patients. Cancer stem cells (CSCs) have been described in MB and represent a sub population characterised by self-renewal and the ability to generate tumour cells, thus representing the reservoir of the tumour. To investigate molecular pathways that characterise this sub population, we isolated CSCs from Sonic Hedgehog Medulloblastoma (SHH MB) arisen in Patched 1 (Ptch1) heterozygous mice, and performed miRNA-and mRNA-sequencing. Comparison of the miRNA-sequencing of SHH MB CSCs with that obtained from cerebellar Neural Stem Cells (NSCs), allowed us to obtain a SHH MB CSC miRNA differential signature. Pathway enrichment analysis in SHH MB CSCs mirnome and transcriptome was performed and revealed a series of enriched pathways. We focused on the putative targets of the SHH MB CSC miRNAs that were involved in the enriched pathways of interest, namely pathways in cancer, PI3k-Akt pathway and protein processing in endoplasmic reticulum pathway. In silico analysis was performed in SHH MB patients and identified several genes, whose expression was associated with worse overall survival of SHH MB patients. This study provides novel candidates whose functional role should be further investigated in SHH MB

Enfezamentos: doenças do milho disseminadas por inseto.

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Incidência do inseto-vetor de molicutes e de enfezamentos em milho.

xTrabalho apresentado no 31º Congresso Paulista de Fitopatologia, 2008, Campinas

Recomendações para o manejo de doenças do milho disseminadas por insetos-vetores.

As doenças do milho disseminadas por insetos-vetores são os enfezamentos causados pelos molicutes espiroplasma e fitoplasma, e as viroses. Entre os insetos-vetores importantes para essa cultura, destacam-se a cigarrinha Dalbulus maidis , que transmite o espiroplasma, o fitoplasma e o vírus da risca (Maize rayado fino virus ), e os pulgões, que transmitem os vírus que causam o mosaico-comum-do-milho. A virose denominada faixa-clorótica-das-nervuras, cujo vírus agente causal é transmitido pela cigarrinha Peregrinus maidis , também ocorre no milho no Brasil, porém de forma esporádica, em baixos níveis de incidência. Os enfezamentos por molicutes e a virose mosaico-comum, eventualmente, têm ocorrido em surtos epidêmicos, causando danos expressivos e, por isso, destacam-se em importância. Este trabalho tem por objetivo caracterizar os enfezamentos, a virose mosaico-comum-do-milho, e os insetos-vetores dos agentes causais dessas doenças. Apresenta evidências da influência de fatores climáticos e de características do sistema de produção na incidência e nos danos que essas doenças podem causar. Apresenta formas possíveis para escape das doenças disseminadas pelos insetos-vetores e alternativas para seu manejo.bitstream/item/121416/1/circ-205.pd