Анализ клеточного состава нативной трансплантационной аутосмеси, используемой для пластики дефектов костной ткани

The cell composition of native transplant autosmes (NTA) used for bone plastics was studied. The histological examination showed the fragments of bone beams with preserved osteoblasts, the foci of myeloid and lymphoid hematopoiesis and the fibrin deposits, which suggested the presence of MMSCs. Immunophenotyping of the NTA cell population revealed a high level of expression of the surface markers CD105, CD73, and CD90 characteristic for MMSC. DNA-flow cytometry of the bone dust confirmed almost complete preservation of graft viability on the 3rd day of culturing (97.7 % of live cells). The data of this study confirm the presence of the osteogenic, osteoinductive, and osteoconductive properties of the bone dust and emphasize the importance of a further study of this-type bone graft for use in surgical interventions.Проведено исследование клеточного состава нативной трансплантационной аутосмеси (НТА), используемой для костной пластики. Гистологическое исследование показало фрагменты костных балок с сохранившимися остеобластами, очаги миелоидного и лимфоидного кроветворения и отложения фибрина, что позволило предположить наличие мультипотентных мезенхимальных стромальных клеток (ММСК). При имммунофенотипировании популяции клеток НТА выявлен высокий уровень экспрессии поверхностных маркеров CD105, CD73 и CD90, характерных для ММСК. ДНК-проточная цитофлоурометрия костной аутосмеси подтвердила практически полное сохранение жизнеспособности трансплантата на третьи сутки культивирования (97,7 % живых клеток). Полученные данные свидетельствуют о наличии у нативной трансплантационной аутосмеси остеогенных, остеоиндуктивных, остеокондуктивных свойств и обосновывают дальнейшее ее исследование с целью использования в качестве аутотрансплантата при хирургических вмешательствах

Internuclear chromosome bridges in thyrocytes of papillary thyroid cancer in patients, subjected to radioactive iodine isotopes during first months after the accident at the Chernobyl nuclear power plant.

Background. Fallout from Chernobyl accident was primarily to iodine radioisotopes, with Iodine-131 (I-131) being the most predominant. Radioiodines accumulated following the accident could induce pathologic changes in thyrocytes. Internuclear chromatine bridges and ‘‘tailed’’nuclei - broken bridge fragments - are considered like cytopathological effects of radiation exposure as these abnormalities are formed from dicentric chromosomes, which are established markers of radiation exposure. Objective. To test the possibility that internuclear bridges and tailed nuclei are cytological markers of radiation exposure of the thyroid. Methods. We investigated thyrocyte nuclear abnormalities in cytological samples from fine-needle aspiration biopsy in papillary thyroid cancer patients exposed to radioiodine after Chernobyl accident (35 subjects from Gomel region, Belarus) and in papillary thyroid cancer of unexposed patients (25 subjects from Leningrad region, Russia). Nuclear abnormalities included internuclear bridges and ‘‘tailed’’ nuclei were examined. Results. Cells in papillary thyroid cancer of irradiated patients are characterized by the high frequency of appearance of hole nucleoplasmic bridges as well as broken bridges in comparison with the control group. The average frequency of thyrocytes with bridges in irradiated patients was almost 4 times higher than that in the unexposed group (4,69±0,69‰ vs. 1,10±0,23 ‰, p<0.001). The same contrast was observed in parameter “frequency of thyrocytes with “tailed” nuclei” (12,40±1,82 ‰ vs 3,68±0,39 ‰, (p<0.001)). Conclusion. Thyrocytes with internuclear bridges may be considered as markers of radiation effects on the thyroid gland

Між'ядерні хромосомні мости в тироцитах при папілярному раку щитоподібної залози у осіб, які зазнали впливу радіоізотопів йоду у перші місяці після аварії на Чорнобильській атомній електростанції.

Background. Fallout from Chernobyl accident was primarily to iodine radioisotopes, with Iodine-131 (I-131) being the most predominant. Radioiodines accumulated following the accident could induce pathologic changes in thyrocytes. Internuclear chromatine bridges and ‘‘tailed’’nuclei - broken bridge fragments - are considered like cytopathological effects of radiation exposure as these abnormalities are formed from dicentric chromosomes, which are established markers of radiation exposure. Objective. To test the possibility that internuclear bridges and tailed nuclei are cytological markers of radiation exposure of the thyroid. Methods. We investigated thyrocyte nuclear abnormalities in cytological samples from fine-needle aspiration biopsy in papillary thyroid cancer patients exposed to radioiodine after Chernobyl accident (35 subjects from Gomel region, Belarus) and in papillary thyroid cancer of unexposed patients (25 subjects from Leningrad region, Russia). Nuclear abnormalities included internuclear bridges and ‘‘tailed’’ nuclei were examined. Results. Cells in papillary thyroid cancer of irradiated patients are characterized by the high frequency of appearance of hole nucleoplasmic bridges as well as broken bridges in comparison with the control group. The average frequency of thyrocytes with bridges in irradiated patients was almost 4 times higher than that in the unexposed group (4,69±0,69‰ vs. 1,10±0,23 ‰, p&lt;0.001). The same contrast was observed in parameter “frequency of thyrocytes with “tailed” nuclei” (12,40±1,82 ‰ vs 3,68±0,39 ‰, (p&lt;0.001)). Conclusion. Thyrocytes with internuclear bridges may be considered as markers of radiation effects on the thyroid gland.Аномалии ядер в виде межъядерных хромосомных мостов формируются из дицентрических хромосом, которые являются маркерами влияния радиационных факторов. Клеточные популяции тироцитов папиллярных раков щитовидной железы у жителей Гомельской области, пострадавших в результате выброса радиоактивных изотопов йода в первые месяцы после аварии на Чернобыльской АЭС, характеризуются повышенной частотой встречаемости межъядерных мостов по сравнению с группой сравнения из Ленинградской области. По-видимому, клоногенные клетки давшие начало злокачественным опухолям у гомельских пациентов могли оказаться генетически мультиаберрантными в результате воздействия радиоактивного йода и иметь повышенную геномную нестабильность, обусловленную циклами «разрыв-слияние-мост».Аномалії ядер у вигляді між'ядерних хромосомних мостів формуються з дицентричних хромосом, які є маркерами впливу радіаційних факторів. Клітинні популяції тироцитів папілярних раків щитовидної залози у мешканців Гомельської області, які постраждали внаслідок викиду радіоактивних ізотопів йоду в перші місяці після аварії на Чорнобильській АЕС, характеризуються підвищеною частотою між'ядерних мостів в порівнянні з групою Ленінградської області. Мабуть, клоногенні клітини, що дали початок злоякісним пухлинам у гомельських пацієнтів, могли виявитися генетично мультиаберантними в результаті впливу радіоактивного йоду і мати підвищену геномну нестабільність, обумовлену циклами «розрив-злиття-міст»

Regeneration of Skin Surface by Multipotent Mesenchymal Stem Cells of Adipose Tissue in Laboratory Animals with Infected Wounds

This paper presents results of experimental studies in laboratory animals with a simulated infected wound, for which mesenchymal stem cells (MSCs) derived from adipose tissue were used in its treatment. The following peculiarities of MSCs for regeneration of skin defects are established: faster arrest of inflammation, accelerated wound healing processes, as well as observed stimulation of growth of skin appendages. The results of this study may serve the basis for further research from development to introduction into clinical practice of cellular technologies for the treatment of infected wound of various etiologies. [Med-Science 2013; 2(2.000): 601-17

Medicine Science 2013;2(2):601-17 Mesenchymal Stem Cells and Skin Regeneration Original Investigation Regeneration of Skin Surface by Multipotent Mesenchymal Stem Cells of Adipose Tissue in Laboratory Animals with Infected Wounds Medicine Science 2013;2(2

Abstract This paper presents results of experimental studies in laboratory animal

Measures of thyroid function among Belarusian children and adolescents exposed to iodine-131 from the accident at the Chernobyl nuclear plant

Background: Thyroid dysfunction after exposure to low or moderate doses of radioactive iodine-131 (131I) at a young age is a public health concern. However, quantitative data are sparse concerning 131I-related risk of these common diseases. Objective: Ou

Measures of thyroid function among Belarusian children and adolescents exposed to iodine-131 from the accident at the Chernobyl nuclear plant

Background: Thyroid dysfunction after exposure to low or moderate doses of radioactive iodine-131 (131I) at a young age is a public health concern. However, quantitative data are sparse concerning 131I-related risk of these common diseases. Objective: Ou

Potential role of tumor-infiltrating T-, B-lymphocytes, tumor-associated macrophages and IgA-secreting plasma cells in long-term survival in the rectal adenocarcinoma patients

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Risk of thyroid follicular adenoma among children and adolescents in Belarus exposed to iodine-131 after the Chornobyl accident.

Several studies reported an increased risk of thyroid cancer in children and adolescents exposed to radioactive iodines, chiefly iodine-131 ((131)I), after the 1986 Chornobyl (Ukrainian spelling) nuclear power plant accident. The risk of benign thyroid tumors following such radiation exposure is much less well known. We have previously reported a novel finding of significantly increased risk of thyroid follicular adenoma in a screening study of children and adolescents exposed to the Chornobyl fallout in Ukraine. To verify this finding, we analyzed baseline screening data from a cohort of 11,613 individuals aged ≤18 years at the time of the accident in Belarus (mean age at screening = 21 years). All participants had individual (131)I doses estimated from thyroid radioactivity measurements and were screened according to a standardized protocol. We found a significant linear dose response for 38 pathologically confirmed follicular adenoma cases. The excess odds ratio per gray of 2.22 (95% confidence interval: 0.41, 13.1) was similar in males and females but decreased significantly with increasing age at exposure (P &lt; 0.01), with the highest radiation risks estimated for those exposed at &lt;2 years of age. Follicular adenoma radiation risks were not significantly modified by most indicators of past and current iodine deficiency. The present study confirms the (131)I-associated increases in risk of follicular adenoma in the Ukrainian population and adds new evidence on the risk increasing with decreasing age at exposure