Cell competition in liver carcinogenesis

Cell competition is now a well-established quality control strategy to optimize cell and tissue fitness in multicellular organisms. While pursuing this goal, it is also effective in selecting against altered/defective cells with putative (pre)-neoplastic potential, thereby edging the risk of cancer development. The flip side of the coin is that the molecular machinery driving cell competition can also be co-opted by neoplastic cell populations to expand unchecked, outside the boundaries of tissue homeostatic control. This review will focus on information that begins to emerge regarding the role of cell competition in liver physiology and pathology. Liver repopulation by normal transplanted hepatocytes is an interesting field of investigation in this regard. The biological coordinates of this process share many features suggesting that cell competition is a driving force for the clearance of endogenous damaged hepatocytes by normal donor-derived cells, as previously proposed. Intriguing analogies between liver repopulation and carcinogenesis will be briefly discussed and the potential dual role of cell competition, as a barrier or a spur to neoplastic development, will be considered. Cell competition is in essence a cooperative strategy organized at tissue level. One facet of such cooperative attitude is expressed in the elimination of altered cells which may represent a threat to the organismal community. On the other hand, the society of cells can be disrupted by the emergence of selfish clones, exploiting the molecular bar codes of cell competition, thereby paving their way to uncontrolled growth

Long-term moderate caloric restriction and social isolation synergize to induce anorexia-like behavior in rats

Moderate caloric restriction (CR) is an effective strategy to delay the onset of chronic disease states. Conversely, social isolation (SI) carries an increased risk of morbidity and mortality from several causes. The present studies were designed to investigate the long-term effect of the two combined exposures. Two-month-old male rats of the Fischer 344 strain were fed either ad libitum or under a regimen of CR, and each of the two animal sets were housed either in group or isolation. Food consumption and animal growth curves were as expected during the first 6 wk of observation. However, starting at 2 mo and continuing until the fifth month of follow up, rats exposed to both CR and SI showed signs of altered feeding behavior and were unable to complete their (already restricted) meal. Furthermore, altered behavior was accompanied by a corresponding decrease in growth rate until no further increase in body weight was observed. Restoration of group-housing conditions led to a reversal of this phenotype. We conclude that chronic moderate CR and SI synergize to induce anorexia-like behavior, representing a simple and reproducible model to study such an eating disorder

Estimasi dan Validasi Total Digestible Nutrient Hijauan Pakan dari Komposisi Kimia Pakan

This study aims to estimate and validate the accuracy of estimated Total Digestible Nutrient (TDN) content in forages based on the chemical composition of feed. Data on nutrient composition for estimates were obtained from the BR-CORTE database consisting of 86 types of forages and primary data from observations consisting of 19 types of forages. Analysis data is used Pearson correlation, multiple linear regression, determination coefficient (R2), and root mean square error (RMSE) for estimation models, variance test (ANOVA), and T-test for validation. The results showed that forage TDN had a negative correlation with Neutral Detergent Fibre (NDF) (P <0.01) and lignin (P <0.05), but was positively correlated with Non-Fibre Carbohydrate (NFC) (P <0.01), Ether Extract (EE) and Crude Protein (CP) (P<0.01). Thus, the TDN value can be estimated from the chemical composition in the feed. The regression equation that will be used to estimate forage TDN content is TDN = 0.482 NDF + 1.538 EE + 0.699 NFC + 0.718 CP (R2=0.994). Then, this equation is validated and compared to the Hartadi’s and Wardeh’s equations. The results of the T-test showed that the TDN value estimated is non significantly different with TDN Hartadi for cattle (P>0.05). It concluded TDN value of local forage can be estimated based on this estimation models.This study aims to estimate and validate the accuracy of estimated Total Digestible Nutrient (TDN) content in forages based on the chemical composition of feed. Data on nutrient composition for estimates were obtained from the BR-CORTE database consisting of 86 types of forages and primary data from observations consisting of 19 types of forages. Analysis data is used Pearson correlation, multiple linear regression, determination coefficient (R2), and root mean square error (RMSE) for estimation models, variance test (ANOVA), and T-test for validation. The results showed that forage TDN had a negative correlation with Neutral Detergent Fibre (NDF) (P <0.01) and lignin (P <0.05), but was positively correlated with Non-Fibre Carbohydrate (NFC) (P <0.01), Ether Extract (EE) and Crude Protein (CP) (P<0.01). Thus, the TDN value can be estimated from the chemical composition in the feed. The regression equation that will be used to estimate forage TDN content is TDN = 0.482 NDF + 1.538 EE + 0.699 NFC + 0.718 CP (R2=0.994). Then, this equation is validated and compared to the Hartadi’s and Wardeh’s equations. The results of the T-test showed that the TDN value estimated is non significantly different with TDN Hartadi for cattle (P>0.05). It concluded TDN value of local forage can be estimated based on this estimation models

Two similar commercial live attenuated AMPV vaccines prepared by random passage of the identical field isolate, have unrelated sequences

Since late \u201880 s Avian metapneumovirus subtype A causes sufficient disease in Europe for commercial companies to have started developing live attenuated vaccines. Here, two of those vaccines were fully consensus sequenced alongside their progenitor field strain (#8544). Sequences comparison shows that the attenuation of field strain #8544 was associated with no common substitutions between the two derived vaccines. This finding suggests that the attenuation of field viruses via serial passage on cell cultures or tissues is the result of a random process, rather than a mechanism aiming to achieve a specific sequence. Furthermore, field vaccination strategies would greatly benefit by the unambiguous vaccine markers identified in this study, enabling a prompt and confident vaccines detection

Determination of Energy and Protein Requirements of Sheep in Indonesia Using a Meta-analytical Approach

The objective of this study was to determine energy and protein requirements, for both maintenance and gain, of sheep in Indonesia by using a meta-analysis method. A database was developed from various in vivo experiments involving sheep as the experimental animals in which energy intake, protein intake and average daily weight gain (ADG) were reported. A total of 38 articles consisting of 137 data points were integrated into the database. Different breeds (Priangan, fat-tailed and local) and sexes (male and female) were specified in the database. Maintenance and gain requirements of dry matter, energy and protein were determined by regressing ADG with dry matter intake (DMI), total digestible nutrient intake (TDNI) and crude protein intake (CPI), respectively. An intercept (where ADG= 0 g/kg MBW/d) and a slope (required nutrient intake per unit ADG) were taken as maintenance and gain requirements, respectively. Results revealed that all sheep breeds had similar energy requirement for maintenance (TDNm). Energy requirement for gain (TDNg) of Priangan breed was lower than other breeds; the breed required 0.860 g TDN for 1 g ADG. Fat-tailed and local breeds required 1.22 and 2.75 g TDN for 1 g ADG, respectively. All breeds also revealed relatively similar protein requirement for maintenance (CPm), i.e. 6.27-6.47 g/kg MBW/d. Priangan breed required less CP for 1 g ADG (CPg), i.e. 0.295 g. Requirements of CPg for fat-tailed and local breeds were 0.336 and 0.497 g/g ADG, respectively. It was concluded that each sheep breed in Indonesia had specific TDN and CP requirements for gain, but similar requirements for maintenance

Feed Potential of Agriculture Waste for Beef Cattle Development in Kuningan Regency, West Java

The aims of research were to identify and analyze potential agricultural waste used as feed, examines the characteristics of beef cattle ranchers and estimate the ability of the addition of beef cattle population in Kuningan Regency. The primary data were taken from interviews with 30 respondents beef cattle farmers selected by purposive sampling in three districts based on the largest beef cattle population as a recommendation by local government of Kuningan Regency Agricultural. Waste samples taken randomly three times to analyze of nutrient composition by proksimat analyze was the type of the most widely used for feed. Secondary data was obtained from Kuningan Regency Veterinary Office, Department of Food Crops and the Central Statistics Agency. The results showed that the type of agricultural waste used in Kuningan Regency from highest to lowest production is rice straw, hay sweet potatoes, peanuts and hay with traditional animal husbandry systems. Districts that can improve beef cattle population from the highest to lowest number was Luragung, Cibingbin, Ciwaru, Subang, Maleber, Cibeureum, Cilebak, Karangkancana and Cimahi. In conclusion, the highest agricultural waste production was rice straw and the highest potential for the development of beef cattle in the Kuningan Regency was Luragung District

Aktivitas Antioksidan Ekstrak Daun Sambiloto (Andrographis Paniculata) dan Ekstrak Kulit Manggis (Garcinia Mangostana) terhadap Aflatoksin B1 pada Jagung: Antioxidant Activity of Andrographis paniculata Leaves Extract and Garcinia mangostana Peel Extract to aflatoxin B1 in Corn

The objective of this study was to analyze the active compound of Garcinia mangostana (GME) and Andrographis paniculata (APE) extracts and the effectiveness of these extracts against aflatoxin B1 (AFB1) which had contaminated corn. The experimental design of this study was a completely randomized factorial design. Factor A was the addition of extract, A0=without addition, A1=addition of 0.08% GME, A2=addition of 0.16% GME, A3=addition of 0.08% APE, A4=addition of 0.16% APE. Factor B was the incubation period, B1=day 0, B2=day-2, B3=day-4. The research variables were extract yield, total phenolic content, antioxidant activity, corn moisture content and gross energy, AFB1 content, production and percentage of inhibition of AFB1. The data obtained were analyzed for  variance and Duncan’s test. The results showed that GME and APE had yields of 16.68% and 5.49%, total phenolic content of 125.28 and 12.62 mg GAE g extract-1 and antioxidant activity of 29.82 and >200 ppm respectively. The addition of extract to each treatment during incubation significantly interacted with AFB1 production. However, the gross energy in each treatment was only affected by the incubation time, while the moisture content increased on the second day to 13.29%. It can be concluded that the addition of 0.16% GME could inhibit the production of AFB1 which was higher than the addition of other extracts during incubation. Key words:        aflatoxin B1, Andrographis paniculata, corn, Garcinia mangostan

In Vitro Digestibility of Ration Containing Different Level of Palm Oil Frond Fermented with Phanerochaetae chrysosporium

Phanerochaete chrysosporium was widely used to delignify agricultural waste product and improve biodegradation of the substrate as animal feed.  The experiment was carried out to increase the use of palm oil fronds as a substitute material for napier grass through biodegradation process with P. chysosporium. A completely randomized design with four treatments and four replications was used. The treatments were ration containing 60% napier grass (R1), ration containing 40% napier grass and 20% fermented palm oil frond (R2), ration containing 20% napier grass and 40% fermented palm oil frond (R3), ration containing 60% fermented palm oil frond (R4). Fourty percent concentrate was included in all treatment rations. Parameters measured were in vitro digestibilities of dry matter, organic matter, crude fiber, NDF, ADF, NH3, TVFA, and ruminal cellulolitic bacteria. Results showed that increasing level of fermented palm oil frond in the ration reduced (P<0.05) digestibilities of dry matter, organic matter, crude fiber, NDF, ADF, N-NH3, TVFA concentration and number of ruminal cellulolytic bacteria. It is concluded that fermentation of palm oil frondwith P. chysosporium decrease lignin content by 47.79%, but increasing the fermented palm oil frond in the ration reduces nutrient digestibilities, N-NH3 and TVFA concentrations and rumen cellulolytic bacteria counts. Fermented palm oil frond up to 40% could be used as a substitute for forages in ruminant rations

Clearance of senescent hepatocytes in a neoplastic-prone microenvironment delays the emergence of hepatocellular carcinoma

Increasing evidence indicates that carcinogenesis is dependent on the tissue context in which it occurs, implying that the latter can be a target for preventive or therapeutic strategies. We tested the possibility that re-normalizing a senescent, neoplastic-prone tissue microenvironment would exert a modulatory effect on the emergence of neoplastic disease. Rats were exposed to a protocol for the induction of hepatocellular carcinoma (HCC). Using an orthotopic and syngeneic system for cell transplantation, one group of animal was then delivered 8 million normal hepatocytes, via the portal circulation. Hepatocytes transplantation resulted in a prominent decrease in the incidence of both pre-neoplastic and neoplastic lesions. At the end of 1 year 50% of control animals presented with HCC, while no HCC were observed in the transplanted group. Extensive hepatocyte senescence was induced by the carcinogenic protocol in the host liver; however, senescent cells were largely cleared following infusion of normal hepatocytes. Furthermore, levels of Il-6 increased in rats exposed to the carcinogenic protocol, while they returned to near control values in the group receiving hepatocyte transplantation. These results support the concept that strategies aimed at normalizing a neoplastic-prone tissue landscape can modulate progression of neoplastic disease