Estimation of total Phenol and flavonoid contents of Citrus limon L Burmf leaves from North Eastern region of India.

Citrus plants belonging to the family Rutaceae, are traditionally used by the local people India to treat scurvy, rheumatism, stomachic, diarrhea, liver disorders and used as an antioxidant. They are valuable source of phenolic and other biologically active compounds.  Phenolic compounds have been reported to have multiple biological effects. In this paper quantitative determination of phenols and flavonoids of Citrus limon L Burmf leaves extracts was carried out using spectrophotometric methods. The plant material was collected from Assam, north eastern region of India. Total phenolic content was determined by Folin-Ciocalteu method and aluminium chloride method was used for flavonoid determination. Acetone and methanol extracts of leaves showed quite high amount of phenol as well as flavinoid contents compared to ethanol and ethyl acetate extracts.  However, all the extracts exhibited significant results. Keywords: Citrus limon, Phenol, Flavonoid, Catechol and Quercetin

Studies on aerial parts of Artemisia pallens wall for phenol, flavonoid and evaluation of antioxidant activity

Herbs have been used for medicinal purposes for centuries. According to recent investigations, they may help reduce the risk of chronic diseases, cardiovascular disease, and cancer due to antioxidant properties, which in turn can be attributed to the various phytoconstituents. With this intention, evaluation of antioxidant activity was performed. Methanol extract of aerial parts of Artemisia pallens Wall was screened for its antioxidant activity due to phenolic and flavonoid contents, by employing radical scavenging assays; 2,2 –diphenyl, 1-picryl hydrazyl (DPPH) and nitric oxide. Ascorbic acid was used as a standard. Quantitative determination of phenols and flavonoids were carried out using spectrophotometric method. Total flavonoid content was determined as quercetin equivalent and total phenolic content was determined as pyrocatechol equivalent using Folin-Ciocalteu reagent. Plant produces more phenolic compounds than flavonoids. IC50 value of methanol extract for DPPH free radical scavenging activity was found to be 292.7 μg, whereas for nitric oxide it was 204.61 μg. The result obtained in the present study indicates that the aerial parts of this plant are a rich source of natural antioxidant