Development of Multi-Analytical Immune-Chromatographic Device for Toxin Indication

Objective of the work was to construct immune-chromatographic device for indication of five toxin types in a single processing cycle and investigation of its capacities regarding environment sample analysis and food testing. Obtained were the conjugates of monoclonal antibodies with nano-particles of colloid gold, formed were membrane composites for sandwich immune-chromatography of separate toxins. On the basis of distinct test panels designed was immune-chromatographic device for multiple analyses, which contained multi-analytical detection element, swab for surface sampling, and storage vessel with test buffer. This device allowed for concurrent identification of botulinic toxins A, B types concentrated up to 5 and 10 ng/ml, respectively; staphylococcal toxin B type - 40 ng/ml, cholera toxin - 500 ng/ml, and ricin - 80 ng/ml, within a single processing cycle. Demonstrated was the possibility of toxin detection in washings from ambient object surfaces, as well as in foodstuffs. Described was preliminary food sample preparation procedure