Assessment of Stability of Chemical Cholera Vaccine in a New Primary Packaging

The bivalent chemical cholera vaccine is the only drug for the prevention of cholera registered in the Russian Federation. The vaccine has been produced in glass bottles containing 210 tablets. At the same time, modern trends dictate the need to produce the drug in varying dispensing and more practical packaging for the convenience of the consumer.The aim of the work was to study the stability of the properties of the immunobiological medicinal product “Bivalent chemical cholera vaccine” with modified filling and in new primary packaging.Materials and methods. When studying the quality of bivalent chemical cholera vaccine batches, physicochemical parameters, formaldehyde content, specific activity and safety, abnormal toxicity, immunogenicity, and microbiological purity were assessed. Stability in terms of “specific activity” was evaluated using dot immunoassay.Results and discussion. As a result of this work, the use of several dispensing options and new primary packaging of cholera vaccine has been experimentally substantiated. The stability of the finished vaccine preparation has been established in the “accelerated aging” test and during long-term storage. The possibility of using dot immunoassay with a conjugate based on staphylococcal protein A, labeled with colloidal gold, to monitor the stability of cholera vaccine has been experimentally demonstrated

Homogeneous deposition of nickel in pores of the ordered thin aluminum oxide

Fabrication of a matrix of ordered vertically oriented nickel nanocolumns with the use of a porous aluminum oxide template is considered. The effect of thinning of a barrier layer at the bottom of the aluminum oxide pores on the nucleation and growth of ordered metal nanocolumns is discussed. Morphological parameters of Ni nanocolumns fabricated in different regimes are studied by atomic force microscopy. Kinetic regulations and conditions of the electrochemical deposition of nickel into a porous aluminum oxide template are investigated