Competence analysis of artificial intelligence professionals

In the conditions of digitalisation, it is necessary to have the appropriate skills. Therefore, many applicants choose occupations in which information technology (IT) and programming knowledge is predominant. Comprehensive measures for preparing graduates in the context of digitalisation cover the formation of appropriate educational programmes. The aim of the study is to provide recommendations for improving the educational process in the field of artificial intelligence (AI) and machine learning based on comparing various programmes affecting the training of professionals in this field. The research uses the following methods. First, it is a sample survey, due to which 4 Russian universities that provide training in programmes in IT, are selected. Second, this is grouping and typology of basic educational curricula, professional standards and disciplines, due to which appropriate typological groupings, are constructed. That allows specifying requirements for knowledge, skills, and abilities necessary for specialists in the field of AI. A comparative analysis of professional standards taking into account the work of a modern specialist with digital skills has been carried out. The competence approach in the educational process has been defined, and the need for a professional standard for IT specialists has been identified. Based on the results obtained, recommendations have been prepared on the basic disciplines that need to be included in the educational programmes of universities to prepare competitive graduates

Statistical analysis of the factors of information society development in the Russian Federation

The article summarises the system of indicators characterising the development of the information society. A generalising characteristic of the subjects of the Russian Federation was given for each of the 23 indicators, which made it possible to identify regions with different fluctuations of the trait. All indicators have been formed into four factors: human capital, innovation potential, information and communication infrastructure and access, and the economic environment. According to the indicators included in each group, the corresponding subindexes have been calculated. Ratings of regions according to the value of sub-indices have been compiled. Based on the values of the sub-indices, the integral indicator characterising the development of the information society has been calculated. The leading and lagging subjects of the Russian Federation in terms of its significance have been identified. In order to identify regions that are homogeneous in terms of the level of development of the information society, their grouping has been carried out

Implementation of digital competencies in higher education educational programs in Russia

In the context of digitalisation, the transformation of the educational process is inevitable and is a priority issue for any country that wants to maintain or improve its position on the world stage. The article presents a comparative analysis of digital competencies and the possibility of their implementation in the higher education system in Russia. The comparison of the formulated competencies in the methodology approved by the Order of the Ministry of Economic Development of the Russian Federation No. 41 dated January 24, 2020, as well as the International Telecommunication Union and other European organisations is considered. The descriptors (knowledge, skills and abilities) of graduates in various fields who, in the era of digitalisation, must have the necessary digital competencies are identified. Today, professional standards, even in the humanities, define the requirements for technical and computer skills. The article proposes formulations of universal competencies in the higher education system based on the analysis

Diversity and occurrence of methylotrophic yeasts used in genetic engineering

Methylotrophic yeasts have been used as the platform for expression of heterologous proteins since the  1980’s. They are highly productive and allow producing eukaryotic proteins with an acceptable glycosylation level.  The first Pichia pastoris-based system for expression of recombinant protein was developed on the basis of the treeexudate-derived strain obtained in the US southwest. Being distributed free of charge for scientific purposes, this system has become popular around the world. As methylotrophic yeasts were classified in accordance with biomolecular  markers, strains used for production of recombinant protein were reclassified as Komagataella phaffii. Although patent  legislation suggests free access to these yeasts, they have been distributed on a contract basis. Whereas their status  for commercial use is undetermined, the search for alternative stains for expression of recombinant protein continues.  Strains of other species of methylotrophic yeasts have been adapted, among which the genus Ogataearepresentatives prevail. Despite the phylogenetic gap between the genus Ogataeaand the genus Komagataellarepresentatives, it turned out possible to use classic vectors and promoters for expression of recombinant protein in all cases. There  exist expression systems based on other strains of the genus Komagataellaas well as the genus Candida. The potential  of these microorganisms for genetic engineering is far from exhausted. Both improvement of existing expression systems and development of new ones on the basis of strains obtained from nature are advantageous. Historically, strains  obtained on the southwest of the USA were used as expression systems up to 2009. Currently, expression systems  based on strains obtained in Thailand are gaining popularity. Since this group of microorganisms is widely represented  around the world both in nature and in urban environments, it may reasonably be expected that new expression systems for recombinant proteins based on strains obtained in other regions of the globe will appear

Gender differences in the pharmacological actions of pegylated glucagon-Like peptide-1 on endothelial progenitor cells and angiogenic precursor cells in a combination of metabolic disorders and lung emphysema

In clinical practice, the metabolic syndrome (MetS) is often associated with chronic obstructive pulmonary disease (COPD). Although gender differences in MetS are well documented, little is known about sex-specific differences in the pathogenesis of COPD, especially when combined with MetS. Consequently, it is not clear whether the same treatment regime has comparable efficacy in men and women diagnosed with MetS and COPD. In the present study, using sodium glutamate, lipopolysaccharide, and cigarette smoke extract, we simulated lipid metabolism disorders, obesity, hyperglycemia, and pulmonary emphysema (comorbidity) in male and female C57BL/6 mice. We assessed the gender-specific impact of lipid metabolism disorders and pulmonary emphysema on angiogenic precursor cells (endothelial progenitor cells (EPC), pericytes, vascular smooth muscle cells, cells of the lumen of the nascent vessel), as well as the biological effects of pegylated glucagon-like peptide 1 (pegGLP-1) in this experimental paradigm. Simulation of MetS/COPD comorbidity caused an accumulation of EPC (CD45−CD31+CD34+), pericytes, and vascular smooth muscle cells in the lungs of female mice. In contrast, the number of cells involved in the angiogenesis decreased in the lungs of male animals. PegGLP-1 had a positive effect on lipids and area under the curve (AUC), obesity, and prevented the development of pulmonary emphysema. The severity of these effects was stronger in males than in females. Furthermore, PegGLP-1 stimulated regeneration of pulmonary endothelium. At the same time, PegGLP-1 administration caused a mobilization of EPC (CD45−CD31+CD34+) into the bloodstream in females and migration of precursors of angiogenesis and vascular smooth muscle cells to the lungs in male animals. Gender differences in stimulatory action of pegGLP-1 on CD31+ endothelial lung cells in vitro were not observed. Based on these findings, we postulated that the cellular mechanism of in vivo regeneration of lung epithelium was at least partly gender-specific. Thus, we concluded that a pegGLP-1-based treatment regime for metabolic disorder and COPD should be further developed primarily for male patients

Использование магнитных наночастиц в биомедицине

The use of nanomaterials offers many advantages due to their unique properties. They can be used as building blocks for the fabrication of various functional diagnostic systems and agents of therapy. This article focuses on the application of magnetic nanoparticles in biomedicine such as magnetic separation, biosensor, contrast agents for MRI (magnetic resonance imaging), local operated hyperthermic treatment of tumors, drug delivery, gene therapy, tissue engineering.Применение наноматералов открывает широкие перспективы благодаря появлению у вещества в наноразмерном состоянии уникальных свойств. В настоящее время на основе наночастиц разрабатывают высокoспецифичные диагностические системы и эффектив ные методы терапии. В обзоре рассмотрены основные направления биомедицинского применения магнитных наночастиц: магнитная сепарация, биосенсоры, контрастирование при MRI-диагностике, управляемая локальная гипертермия опухолей, целевая доставка препаратов, генотерапия, а также конструирование тканей

Investigation on Electrochemical Interface Between Li1.5Al0.5Ge1.5(PO4)3 Glass-Ceramics and Composite Anode Based on Li4Ti5O12

Твердые электролиты состава Li1.5Al0.5Ge1.5(PO4)3 получены методом направленной кристаллизации монолитного стекла. Порошок Li4Ti5O12 синтезирован растворным методом. Исследовано межфазное сопротивление композиционного анода Li4Ti5O12, содержащего 5 мас.% Li3BO3, с твердым электролитом Li1.5Al0.5Ge1.5(PO4)3 методом электрохимического импеданса. Установлено, что увеличение температуры припекания приводит к уменьшению межфазного сопротивления. Обнаружено, что сопротивление ячеек, отожженных при 800 °C, составляет 13,1 kОм см2 при 80 °C.Li1.5Al0.5Ge1.5(PO4)3 solid electrolytes were obtained by crystallization of monolithic glass. Li4Ti5O12 powder was synthesized by the solution method. The interfacial resistance of a composite Li4Ti5O12 anode containing 5 wt% Li3BO3 with a solid electrolyte Li1.5Al0.5Ge1.5(PO4)3 was investigated by the electrochemical impedance. It was found that the annealing temperature growth leads to the decrease of interphase resistance. It was established that the resistance of cells annealed at 800 °C is 13.1 kΩ cm2 at 80 °C.Благодарности: исследование выполнено при государственной поддержке молодых российских ученых (стипендия Президента РФ СП-604.2021.1). Работа выполнена с использованием оборудования ЦКП «Состав вещества» ИВТЭ УрО РАН.Acknowledgments: the research was supported by the Council of the President of the Russian Federation (scholarship №СП-604.2021.1). The characterization of materials was carried out at the Shared Access Centre “Composition of Compounds” of the Institute of High Temperature Electrochemistry of the Ural Branch of the RAS

Production of subtilisin proteases in bacteria and yeast

In this review, we discuss the progress in the study and modif ication of subtilisin proteases. Despite longstanding applications of microbial proteases and a large number of research papers, the search for new protease genes, the construction of producer strains, and the development of methods for their practical application are still relevant and important, judging by the number of citations of the research articles on proteases and their microbial producers. This enzyme class represents the largest share of the industrial production of proteins worldwide. This situation can explain the high level of interest in these enzymes and points to the high importance of designing domestic technologies for their manufacture. The review covers subtilisin classif ication, the history of their discovery, and subsequent research on the optimization of their properties. An overview of the classes of subtilisin proteases and related enzymes is provided too. There is a discussion about the problems with the search for (and selection of) subtilases from natural strains of various microorganisms, approaches to (and specifics of) their modif ication, as well as the relevant genetic engineering techniques. Details are provided on the methods for expression optimization of industrial subtilases of various strains: the details of the most important parameters of cultivation, i. e., composition of the media, culture duration, and the inf luence of temperature and pH. Also presented are the results of the latest studies on cultivation techniques: submerged and solid-state fermentation. From the literature data reviewed, we can conclude that native enzymes (i. e., those obtained from natural sources) currently hardly have any practical applications because of the decisive advantages of the enzymes modified by genetic engineering and having better properties: e. g., thermal stability, general resistance to detergents and specif ic resistance to various oxidants, high activity in various temperature ranges, independence from metal ions, and stability in the absence of calcium. The vast majority of subtilisin proteases are expressed in producer strains belonging to different species of the genus Bacillus. Meanwhile, there is an effort to adapt the expression of these enzymes to other microbes, in particular species of the yeast Pichia pastoris

Wheat-barley hybridization – the last forty years

Abstract Several useful alien gene transfers have been reported from related species into wheat (Triticum aestivum), but very few publications have dealt with the development of wheat/barley (Hordeum vulgare) introgression lines. An overview is given here of wheat 9 barley hybridization over the last forty years, including the development of wheat 9 barley hybrids, and of addition and translocation lines with various barley cultivars. A short summary is also given of the wheat 9 barley hybrids produced with other Hordeum species. The meiotic pairing behaviour of wheat 9 barley hybrids is presented, with special regard to the detection of wheat– barley homoeologous pairing using the molecular cytogenetic technique GISH. The effect of in vitro multiplication on the genome composition of intergeneric hybrids is discussed, and the production and characterization of the latest wheat/barley translocation lines are presented. An overview of the agronomical traits (b-glucan content, earliness, salt tolerance, sprouting resistance, etc.) of the newly developed introgression lines is given. The exploitation and possible use of wheat/barley introgression lines for the most up-to-date molecular genetic studies (transcriptome analysis, sequencing of flow-sorted chromosomes) are also discussed

Hemozoin "knobs" in Opisthorchis felineus infected liver

Background Hemozoin is the pigment produced by some blood-feeding parasites. It demonstrates high diagnostic and therapeutic potential. In this work the formation of co-called hemozoin “knobs” – the bile duct ectasia filled up by hemozoin pigment - in Opisthorhis felineus infected hamster liver has been observed. Methods The O. felineus infected liver was examined by histological analysis and magnetic resonance imaging (MRI). The pigment hemozoin was identified by Fourier transform infrared spectroscopy and high resolution electrospray ionization mass spectrometry analysis. Hemozoin crystals were characterised by high resolution transmission electron microscopy. Results Hemozoin crystals produced by O. felineus have average length 403 nm and the length-to-width ratio equals 2.0. The regurgitation of hemozoin from parasitic fluke during infection leads to formation of bile duct ectasia. The active release of hemozoin from O. felineus during in vitro incubation has also been evidenced. It has been shown that the hemozoin knobs can be detected by magnetic resonance imaging. Conclusions In the paper for the first time the characterisation of hemozoin pigment extracted from liver fluke O. felineus has been conducted. The role of hemozoin in the modification of immune response by opisthorchiasis is assumed