Ischemia modified albumin and thiol/disulfide balance in patients with Hashimoto’s thyroiditis

Hashimoto thyroiditis is a common cause of goiter and acquired hypothyroidism in individuals residing in areas of no iodine deficiency. The fact that the structure of serum albumin exhibits changes in ischemic conditions has paved the way for the discovery of a new serum cardiac ischemia marker, Ischemia Modified Albumin. The other one, thiol/disulphide homeostasis, plays an important part in antioxidative protection, detoxification, cell growth, and apoptosis. In this study, we aimed to investigate both the relationship between Thiol/Disulphide homeostasis and Ischemia Modified Albumin in patients diagnosed with Hashimoto’s Thyroiditis. A total of 70 Hashimoto’’s thyroiditis patients and 50 healthy ones were included in this study. Age, gender, thyroid-stimulating hormone (TSH), anti-thyroid peroxidase (TPO), anti-thyroglobulin (TG) levels were recorded. Ischemia Modified Albumin and thiol-disulphid homeostasis parameters were measured through automated spectrophotometric methods. The ages of individuals included in the study ranged from 35 to 58 years. The native thiol/total thiol were found to be significantly lower in Hashimoto patients when compared to those enrolled in the control group (P < 0.05), whereas the Ischemia Modified Albumin, disulphide, native thiol, total thiol, disulphide/native thiol, and disulphide/total thiol were found to be significantly higher in Hashimoto patients when compared to those in the control (P < 0.05). Increased Ischemia Modified Albumin, native and total thiol, and disulphide levels are related to increased oxidative stress. Although Ischemia Modified Albumin and Thiol-disulphide defense are important oxidative indicators in Hashimoto’s Thyroiditis, many determinants are known to be involved in this process

Production of hydroxyapatite–bacterial cellulose composite scaffolds with enhanced pore diameters for bone tissue engineering applications

2-s2.0-85074012252Abstract: Bone tissue engineering scaffolds used for the treatment of bone defects are required to be osteoconductive, osteoinductive, osteogenic, biocompatible, and have enough porosity to allow osteointegration, as well as vascularization. It is known that addition of the hydroxyapatite (HAp) to bone tissue scaffolds promotes bone formation by increasing osteoconductivity. Bacterial cellulose (BC) is a highly biocompatible material, and its mechanical properties and fibrous structure allow that it can be used as a bone tissue scaffold; yet, the nano-porous structure of BC (50–200 nm) prevents or limits cell migration and vascularization. In this study, it is intended to take advantage of the porous structure and mechanical strength of BC and osteoconductive properties of HAp for the production of tissue engineering scaffolds. Pore sizes of BC were enhanced to 275 ?m by a novel shredded agar technique, and SaOs-2 cells were shown to migrate between the fibers of the modified BC. It was observed that mineralization of SaOs-2 cells was enhanced on in situ produced HAp-BC nano-composites compared to BC scaffolds. Graphic abstract: [Figure not available: see fulltext.] © 2019, Springer Nature B.V.13FBE008, 2010K120810 113M243 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAKThis work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) through COST project (113M243) and TUBITAK 2211-C Domestic Graduate Scholarship Program and Ege University Scientific Research Projects Council (13FBE008) and Republic of Turkey Ministry of Development [EGE MATAL; 2010K120810]. The authors thank Ko? University Research Center for Translational Medicine (KUTTAM) and Assist. Prof. Ser?in Karah?seyino?lu for the use of the confocal microscopy.This work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) through COST project (113M243) and TUBITAK 2211-C Domestic Graduate Scholarship Program and Ege University Scientific Research Projects Council (13FBE008) and Republic of Turkey Ministry of Development [EGE MATAL; 2010K120810]. The authors thank Koç University Research Center for Translational Medicine (KUTTAM) and Assist. Prof. Serçin Karahüseyinoğlu for the use of the confocal microscopy

The effects of different intensities, frequencies and exposure times of extremely low-frequency electromagnetic fields on the growth of Staphylococcus aureus and Escherichia coli O157:H7

PubMed ID: 24279632The impact of different types of extremely low-frequency electromagnetic fields (ELF-EMF) on the growth of Staphylococcus aureus and Escherichia coli O157:H7 was investigated. The cultures of bacteria in broth media were exposed to sinusoidal homogenous ELF-EMF with 2 and 4mT magnetic intensities. Each intensity for each bacteria was combined with three different frequencies (20, 40 and 50 Hz), and four different exposure times (1, 2, 4 and 6 h). A cell suspension of each experiment was diluted for the appropriate range and inoculated to Mueller-Hinton Agar (MHA) plates after exposure to ELF-EMF. The number of colony forming units (CFU) of both strains was obtained after incubation at 37°C for 24 h. Data were statistically evaluated by one-way analysis of variance (ANOVA), statistical significance was described at p<0.05 and data were compared with their non-exposed controls. Magnetic intensity, frequency and exposure time of ELF-EMFs changed the characteristic responses for both microorganisms. Samples exposed to ELF-EMF showed a statistically significant decrease compared to their controls in colony forming capability, especially at long exposure times. An exposure to 4mT-20 Hz ELF-EMF of 6 h produced maximum inhibition of CFU compared to their controls for both microorganisms (95.2% for S. aureus and 85% for E. coli). © 2015 Informa Healthcare USA, Inc.This work supported by The Scientific and Technological Research Council of Turkey (TUBITAK) within the funding programme 2209. The authors declare no conflict of interests. The authors alone are responsible for the content and writing of the article. -

Optimization of bacterial cellulose production by Gluconacetobacter xylinus using carob and haricot bean

International Biomedical Engineering Congress -- 2015 -- Near E Univ, North Nicosia, CYPRUSWOS: 000380626900002PubMed ID: 26906562Bacterial cellulose (BC) can be used in medical, biomedical, electronic, food, and paper industries because of its unique properties distinguishing it from plant cellulose. BC production was statistically optimized by Gluconacetobacter xylinus strain using carob and haricot bean (CHb) medium. Eight parameters were evaluated by Plackett-Burman Design and significant three parameters were optimized by Central Composite Design. Optimal conditions for production of BC in static culture were found as: 2.5 carbon source, 2.75 g/L protein source, 9.3% inoculum ratio, 1.15 g/L. citric acid, 2.7 g/L Na2HPO4, 30 degrees C incubation temperature, 5.5 initial pH, and 9 days of incubation. This study reveals that BC production can be carried out using carob and haricot bean extracts as carbon and nitrogen sources, and CHb medium has higher buffering capacity compared to Hestrin and Schramm media. Model obtained from this study is used to predict and optimize BC production yield using CHb medium. (C) 2016 Elsevier B.V. All rights reserved

Five new cardenolides transformed from oleandrin and nerigoside by Alternaria eureka 1E1BL1 and Phaeosphaeriasp. 1E4CS-1 and their cytotoxic activities

2-s2.0-85098151251Biotransformation of oleandrin (1) and nerigoside (2) by endophytic fungi; Alternaria eureka 1E1BL1 and Phaeospheria sp. 1E4CS-1, has led to the isolation of five new metabolites (3, 5, 6, 7 and 8) together with a known compound (4). The structures of the biotransformation products were elucidated by 1D-, 2D NMR and HR-MS. Phaeospheria sp. mainly provided monooxygenation reactions on the A and B rings, whereas A. eureka afforded both monooxygenated and desacetylated derivatives of the substrates. Cytotoxic activity of the compounds was tested against a non-cancerous (HEK-293) and four cancer (PANC-1, MIA PaCa-2, DU 145 and A549) cell lines by MTT cell viability assay. All compounds were less cytotoxic than oleandrin, which had IC50 values ranging between 2.7 and 41.9 nM. Two of the monohydroxylated metabolites, viz. 7(?)-hydroxy oleandrin (3) and 1(?)-hydroxy oleandrin (7), were also potent with IC50 values from 18.45 to 39.0 nM, while desacetylated + monohydroxylated, or dihydroxylated products had much lower cytotoxicity. Additionally, the lesser activity of 2 and its metabolite (6) possessing diginose as sugar residue inferred that oleandrose moiety is important for the toxicity of oleandrin as well as hydrophobicity of the steroid core. © 2020 Phytochemical Society of Europe152Z118 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAKThis project was supported by The Scientific and Technological Research Council of Turkey (TUBITAK, Project No: 152Z118). We are thankful to Dr. Özge Özşen for her assistance in preliminary experiments