Usage of Solid Medium on the Basis of Corn-Steep Extract Hydrolysate in Manufacturing of Live Plague Vaccine and for Plague Agent Strain Preservation

Objective of the study was to develop a solid medium on the basis of enzyme digest of corn-steep extract for manufacturing of live plague vaccine and storage of plague agent strains. Materials and methods. Vaccine strain and virulent strains of Yersinia pestis, nutrient media for accumulation and storage. Investigated parameters were assessed according to regulatory documentation. Results and conclusions. Developed has been nutrient medium based on enzyme digest of corn-steep extract with growth stimulation additives – Mohr’s salt and sodium sulphite. Studied have been its physical-chemical and biological properties. Approbation of the medium in manufacturing laboratory has revealed its high efficiency and possibility of usage in industrial production of live plague vaccine. Batches of preparation with optical concentration of 100 mlrd/ml and (68.2±0.9) % viability have been manufactured. Application of the stated medium allows for increase in biomass output and decrease in prime cost of final product. Confirmed has been the possibility to store the virulent plague agent strains on the medium at (4±2) °C for 18 months without reduction of the culture viability

ORGANIZATION OF ANTI-EPIDEMIC MEASURES DURING THE ANTHRAX OUTBREAK IN THE YAMALO-NENETS AUTONOMOUS DISTRICT IN 2016

The organizational peculiarities of anti-epidemic measures during the anthrax outbreak in the Yamalo-Nenets Autonomous District in 2016 are presented. Complex of these measures provided for anthrax patients active identification and hospitalization, preventive immunization and emergency antibiotic prophylaxis of risk groups, vaccination of reindeer, utilization of fallen animals. Disinfection, deratization and desinsection measures were performed. Native residents were evacuated from infection focus and sensitization campaign among the population was carried out. Organized were sanitary inspection stations and temporary accommodation points. Due to operational implementation of anti-epidemic measures in the interagency format the anthrax focus was localized within one incubation period

DETERMINATION OF PHYLOGENETIC RELATIONSHIP OF YERSINIA PESTIS STRAINS FROM NATURAL PLAGUE FOCI OF THE CAUCASUS BY MULTI-LOCUS VNTR-ANALYSIS

Aim. Determination of the degree of phylogenetic relationship of Yersinia pestis strains isolated from the territories of natural foci of plague from the Caucasus using VNTR-typing by 25 loci (MLVA25). Materials and methods. 26 strains of Y. pestis from Russian natural foci of the Caucasus were used in the study. 25 loci of tandem repeats in Y. pestis genome by Le Fleche scheme were used for execution of multi-locus VNTR-analysis. Deciphering of nucleotide sequences was carried out in automatic sequencer ABI 3130 Genetic Analyser. Analysis of confinement of clusters to certain territories, objects and time of isolation of strains was carried out using Arc GIS 10.1 program. Results. Groups of MLVA25-types of various levels of discrimination were formed: clusters, groups and subgroups. Clusters were formed by strains of various taxonomic membership: main and subspecies of Y. pestis. Subgroups reflect membership of strains in certain foci, and MLVA25-types - the degree of genetic relationship. Conclusion. Genetic «portraits» of plague causative agents obtained using MLVA25-types circulating in various natural-focal territories allow to solve problems ofboth theoretical and practical character: from interpretation of microevolution processes to the search of the source of infection and ways of its spread during possible epidemic complications