Atomic Force Microscopy Study of Nano-Physiological Response of Ladybird Beetles to Photostimuli

Background: Insects are of interest not only as the most numerous and diverse group of animals but also as highly efficient bio-machines varying greatly in size. They are the main human competitors for crop, can transmit various diseases, etc. However, little study of insects with modern nanotechnology tools has been done. Methodology/Principal Findings: Here we applied an atomic force microscopy (AFM) method to study stimulation of ladybird beetles with light. This method allows for measuring of the internal physiological responses of insects by recording surface oscillations in different parts of the insect at sub-nanometer amplitude level and sub-millisecond time. Specifically, we studied the sensitivity of ladybird beetles to light of different wavelengths. We demonstrated previously unknown blindness of ladybird beetles to emerald color (,500nm) light, while being able to see UV-blue and green light. Furthermore, we showed how one could study the speed of the beetle adaptation to repetitive flashing light and its relaxation back to the initial stage. Conclusions: The results show the potential of the method in studying insects. We see this research as a part of what might be a new emerging area of ‘‘nanophysiology’ ’ of insects

О наступании ледников в условиях вулканической деятельности вулкана Ключевской (Камчатка)

New data on continuing advance of the Kamchatka glaciers Erman, Vlodavets, Sopochny, Schmidt, and Bogdanovich were obtained as a result of analysis of aerospace information taken at different times. Glacier Erman advances during the past 70  years (1945–2016). Over the period from 1949 to 2016, its area increased by 4.7 km2 and the length – by 3.3 km (from 18.2 to 21.5 km). The highest speed of advancing had been estimated for the Schmidt glacier, in 2007–2013 it exceeded 100 m/year. We had identified a new isolated glacier between glaciers Erman and Schmidt, named as the Obvalny (the «Avalanche») glacier, since in 1945 this glacier was buried under volcanic-avalanche deposits resulted from the Klyuchevskaya volcano eruption. In 1975–2016, the Obvalny glacier advanced over a distance of about 1700 m. Also, we had found more 30 ice-rock massifs («wandering glaciers») on slopes of the Klyuchevskaya volcano. Their contours looked like drops or tongues, and some of them advanced in the frontal zone. From 2002 to 2016, the «wandering glacier» in the upper reaches of river Glubokaya (eastern sector of the volcano) advanced for 740 m (55 m/year). Advancing of glaciers here is a consequence of lateral and terminal eruptions of the Klyuchevskaya volcano in XX and XXI centuries. Erman Glacier, glacier advance, Klyuchevskaya volcano, lateral eruption, space images, volcanic and rock avalanche deposits, «wandering glaciers».На основе анализа разновременной аэрокосмической информации за период 1949–2016 гг. полу- чены новые данные о современном состоянии и наступании ледников района вулкана Ключев- ской – Эрмана, Влодавца, Сопочного, Шмидта, Богдановича и безымянных «блуждающих ледников». Площадь ледника Эрмана увеличилась на 4,7 км2, а длина достигла 21,5 км. Самые высокие темпы наступания – до 100 м/год – характерны для ледника Шмидта. Наступание ледников есть следствие латеральных и терминальных извержений вулкана Ключевской в XX и XXI вв

On advancing of glaciers due to activity of the Klyuchevskaya Sopka volcano (Kamchatka)

New data on continuing advance of the Kamchatka glaciers Erman, Vlodavets, Sopochny, Schmidt, and Bogdanovich were obtained as a result of analysis of aerospace information taken at different times. Glacier Erman advances during the past 70  years (1945–2016). Over the period from 1949 to 2016, its area increased by 4.7 km2 and the length – by 3.3 km (from 18.2 to 21.5 km). The highest speed of advancing had been estimated for the Schmidt glacier, in 2007–2013 it exceeded 100 m/year. We had identified a new isolated glacier between glaciers Erman and Schmidt, named as the Obvalny (the «Avalanche») glacier, since in 1945 this glacier was buried under volcanic-avalanche deposits resulted from the Klyuchevskaya volcano eruption. In 1975–2016, the Obvalny glacier advanced over a distance of about 1700 m. Also, we had found more 30 ice-rock massifs («wandering glaciers») on slopes of the Klyuchevskaya volcano. Their contours looked like drops or tongues, and some of them advanced in the frontal zone. From 2002 to 2016, the «wandering glacier» in the upper reaches of river Glubokaya (eastern sector of the volcano) advanced for 740 m (55 m/year). Advancing of glaciers here is a consequence of lateral and terminal eruptions of the Klyuchevskaya volcano in XX and XXI centuries. Erman Glacier, glacier advance, Klyuchevskaya volcano, lateral eruption, space images, volcanic and rock avalanche deposits, «wandering glaciers»