CHANGE IN CONTENT OF GLYCOPROTEINS ON THE SURFACE OF ENDOTHELIAL CELL CULTURE EA.HY 926 AND INTIMA OF INTERNAL CAROTID ARTERIES UNDER THE INFLUENCE OF MAGNESIUM OROTATE

Aim. To study changes in the glycoproteins content in the glycocalyx of endothelial cells under the influence of magnesium orotate.Material and methods. Endotheliocytes of cell culture EA.hy 926 were examined before  and after addition of the magnesium orotate to the culture medium. Intimal endothelium of internal carotid arteries, fragments of which were obtained by sequential execution of bilateral resection of the arteries before  and after course of treatment with magnesium orotate was also investigated. Anthony’ method (1931), designed for the study of glycoproteins in the bacteria capsule and adapted by L.V. Didenko (2013) for eukaryotes was used to detect glycoproteins. The scanning electron microscopy with simultaneous x-ray microanalysis was applied.Results. Total number of Cu2+-signals per one scanning field in the mapping of samples for Cu2+ as the indirect index of proteoglycan content in EA.hy 926 endothelial cell culture before incubation with magnesium orotate was 6928±124, and after incubation with magnesium orotate – 7592±131. Number of Cu2+-signals on the surface of the internal carotid artery intima before treatment with magnesium orotate was 5015±407, and after treatment – 6100±152 per one scanning field.Conclusion. A significant increase in the content of glycoproteins on the surface of EA.hy926 endothelial cell culture (+10%) and internal carotid artery intima (+22%) under the influence of magnesium orotate was found

Effect of magnesium on the cardiovascular system in women

Recent scientific data on the influence of magnesium on the cardiovascular and reproductive system in women is summarized. Evidence-based medicine data has shown a link between magnesium blood (plasma, red blood cells) levels and increased risk of somatic diseases in women of reproductive age. Hypomagnesemia increases the risk of sudden cardiac death, unstable angina, and cardiovascular mortality due to myocardial tissue functional disorders, mitochondrial failure. Magnesium ions are essential for maintaining the physiological pregnancy. Experimental and clinical data allows using drugs of magnesium in preventing and treating heart disease in women. Their use is also possible for treatment of gynecological diseases, for pregnant women with arterial hypertension and connective tissue dysplasia

MORPHOFUNCTIONAL CHARACTERISTICS OF FIBROBLASTS (MCCOY CELL LINE) CULTURED WITH MAGNESIUM PREPARATIONS

Aim. To study the effect of magnesium orotate, magnesium/pyridoxine combination and magnesium sulfate on fibroblast morphofunctional characteristics in cell culture of fibroblasts (McCoy line).Material and methods. The study of fibroblasts (McCoy line) with the addition of magnesium-containing preparations (magnesium orotate, magnesium/pyridoxine combination, magnesium sulphate) to the culture medium was performed using scanning and transmission electron microscopy.Results. When adding into the media magnesium orotate or magnesium/pyridoxine combination, significant changes in morphofunctional state of fibroblasts were noted, which were absent when adding magnesium sulfate. At that fibroblasts significantly promoted synthetic and secretory activity. This was expressed in the formation of amorphous and fibrous components in well-formed cell layers.Conclusion. Stimulation by magnesium ions of the proliferative activity of fibroblasts is shown in the morphological analysis of the effect of magnesium orotate or magnesium in combination with pyridoxine and magnesium sulfate on morphological and functional organization of fibroblasts. Revealed the predominant influence of magnesium orotate, and a less pronounced effect of magnesium in combination with pyridoxine on biosynthetic processes in the cells, including the synthesis of amorphous and fibrous components (protocollagen and elastin) of the extracellular matrix founded.</p