10 research outputs found

    Stomoxys calcitrans (Diptera: muscidae): срСдства ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Ρ‹ Π±ΠΎΡ€ΡŒΠ±Ρ‹. ΠžΠ±Π·ΠΎΡ€

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    The purpose of the research: to analyze and summarize literature data on the main means and methods of protecting animals from stable flies Stomoxys calcitrans and to reduce their number.Materials and methods. The analysis of literature data from foreign sources over the past 30 years describing the means and methods of controlling the numbers of S. calcitrans and ways to protect animals from their attack. Results and discussion. A literature review on the means and methods of controlling the abundance of S. calcitrans is presented. Chemicals are widespread and can be used to combat the pre-adult stages of development of flies by introducing them into the substrate or feeding animals, as well as to combat adult insects by treating surfaces or in traps. In the description of physical control methods, attention is paid to traps and various methods of attracting insects to them. As biological control methods for S. calcitrans, researchers are actively studying the possibilities and prospects of using parasitoids (for example, Hymenoptera, including four families and about ten species), more than ten species of ticks from different families, entomopathogenic nematodes (a species of Heterorhabditis bacteriophora), fungi and individual species bacteria. ЦСль исслСдований: провСсти Π°Π½Π°Π»ΠΈΠ· ΠΈ ΠΎΠ±ΠΎΠ±Ρ‰Π΅Π½ΠΈΠ΅ Π»ΠΈΡ‚Π΅Ρ€Π°Ρ‚ΡƒΡ€Π½Ρ‹Ρ… Π΄Π°Π½Π½Ρ‹Ρ… ΠΎΠ± основных срСдствах ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Π°Ρ… Π·Π°Ρ‰ΠΈΡ‚Ρ‹ ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ… ΠΎΡ‚ осСнних ΠΆΠΈΠ³Π°Π»ΠΎΠΊ Stomoxys calcitrans ΠΈ сниТСния ΠΈΡ… числСнности.ΠœΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Ρ‹ ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Ρ‹. ΠŸΡ€ΠΎΠ²Π΅Π΄Π΅Π½ Π°Π½Π°Π»ΠΈΠ· Π»ΠΈΡ‚Π΅Ρ€Π°Ρ‚ΡƒΡ€Π½Ρ‹Ρ… Π΄Π°Π½Π½Ρ‹Ρ… ΠΈΠ· Π·Π°Ρ€ΡƒΠ±Π΅ΠΆΠ½Ρ‹Ρ… источников Π·Π° послСдниС 30 Π»Π΅Ρ‚, ΠΎΠΏΠΈΡΡ‹Π²Π°ΡŽΡ‰ΠΈΡ… срСдства ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Ρ‹ контроля числСнности S. calcitrans ΠΈ способы Π·Π°Ρ‰ΠΈΡ‚Ρ‹ ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ… ΠΎΡ‚ ΠΈΡ… нападСния. Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹ ΠΈ обсуТдСниС. ΠŸΡ€Π΅Π΄ΡΡ‚Π°Π²Π»Π΅Π½ Π»ΠΈΡ‚Π΅Ρ€Π°Ρ‚ΡƒΡ€Π½Ρ‹ΠΉ ΠΎΠ±Π·ΠΎΡ€ ΠΎ срСдствах ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Π°Ρ… контроля числСнности S. calcitrans. Π₯имичСскиС срСдства ΡˆΠΈΡ€ΠΎΠΊΠΎ распространСны ΠΈ ΠΌΠΎΠ³ΡƒΡ‚ Π±Ρ‹Ρ‚ΡŒ ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΠΎΠ²Π°Π½Ρ‹ для Π±ΠΎΡ€ΡŒΠ±Ρ‹ с ΠΏΡ€Π΅Π΄ΠΈΠΌΠ°Π³ΠΈΠ½Π°Π»ΡŒΠ½Ρ‹ΠΌΠΈ стадиями развития ΠΌΡƒΡ… ΠΏΡƒΡ‚Π΅ΠΌ внСсСния Π² субстрат ΠΈΠ»ΠΈ скармливания ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹ΠΌ, Π° Ρ‚Π°ΠΊΠΆΠ΅ для Π±ΠΎΡ€ΡŒΠ±Ρ‹ со взрослыми насСкомыми ΠΏΡƒΡ‚Π΅ΠΌ ΠΎΠ±Ρ€Π°Π±ΠΎΡ‚ΠΊΠΈ повСрхностСй ΠΈΠ»ΠΈ Π² Π»ΠΎΠ²ΡƒΡˆΠΊΠ°Ρ…. ΠŸΡ€ΠΈ описании физичСских ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠ² Π±ΠΎΡ€ΡŒΠ±Ρ‹ ΡƒΠ΄Π΅Π»Π΅Π½ΠΎ Π²Π½ΠΈΠΌΠ°Π½ΠΈΠ΅ Π»ΠΎΠ²ΡƒΡˆΠΊΠ°ΠΌ ΠΈ Ρ€Π°Π·Π»ΠΈΡ‡Π½Ρ‹ΠΌ способам привлСчСния ΠΊ Π½ΠΈΠΌ насСкомых. Π’ качСствС биологичСских ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠ² контроля S. calcitrans исслСдоватСли Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎ ΠΈΠ·ΡƒΡ‡Π°ΡŽΡ‚ возмоТности ΠΈ пСрспСктивы примСнСния ΠΏΠ°Ρ€Π°Π·ΠΈΡ‚ΠΎΠΈΠ΄ΠΎΠ² (Π½Π°ΠΏΡ€ΠΈΠΌΠ΅Ρ€, ΠΏΠ΅Ρ€Π΅ΠΏΠΎΠ½Ρ‡Π°Ρ‚ΠΎΠΊΡ€Ρ‹Π»Ρ‹Ρ…, Π²ΠΊΠ»ΡŽΡ‡Π°ΡŽΡ‰ΠΈΡ… Ρ‡Π΅Ρ‚Ρ‹Ρ€Π΅ сСмСйства ΠΈ ΠΎΠΊΠΎΠ»ΠΎ дСсяти Π²ΠΈΠ΄ΠΎΠ²), Π±ΠΎΠ»Π΅Π΅ дСсяти Π²ΠΈΠ΄ΠΎΠ² ΠΊΠ»Π΅Ρ‰Π΅ΠΉ ΠΈΠ· Ρ€Π°Π·Π½Ρ‹Ρ… сСмСйств, энтомопатогСнных Π½Π΅ΠΌΠ°Ρ‚ΠΎΠ΄ (Π²ΠΈΠ΄ Heterorhabditis bacteriophora), Π³Ρ€ΠΈΠ±ΠΎΠ² ΠΈ ΠΎΡ‚Π΄Π΅Π»ΡŒΠ½Ρ‹Ρ… Π²ΠΈΠ΄ΠΎΠ² Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠΉ

    Detection of genetic material of causative agents of animal viral diseases in blood-sucking dipterans from the Tyumen Region

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    The purpose of the research is to test blood-sucking dipterans collected in the Tyumen Region for genetic material of viruses that cause dangerous diseases in live-stock animals.Materials and methods. From May to October 2021, blood-sucking insects of the Diptera order whose species membership was established by tabular keys were collected on pastures and farms in ten Tyumen Region districts. In 60 samples formed from the captured insects according to the taxonomic affiliation and the period and place of collection, the presence of genetic material of the leukemia provirus and the dermatitis nodularis virus of cattle (bovine) and the African swine fever virus was evaluated by polymerase chain reaction (PCR) in real time.Results and discussion. Adult insects collected for the PCR analysis were blood-sucking flies (family Muscidae, genus Stomoxys), mosquitoes (family Culicidae, genus Aedes), midges (family Simuliidae, genera Byssodon and Schoenbaueria), horseflies (family Tabanidae, genera Hybomitra, Tabanus and Haematopota), and biting midges (family Ceratopogonidae, genus Culicoides). As a result of the PCR testing of the samples for the bovine leukemia provirus DNA, 1 out of 13 samples of Stomoxys spp. (7.7%) and 1 of 13 samples of Hybomitra spp. (7.7%) were positive. The bovine leukemia provirus DNA detected in blood-sucking insects indicates the presence of this pathogen in the insect collection area as well as their possible involvement in its spread. Further research is needed on the Stomoxys spp. and Hybomitra spp. vector competence in vivo, considering natural and climatic features of the Tyumen Region

    ΠžΡΡ‚Ρ€Π°Ρ Ρ‚ΠΎΠΊΡΠΈΡ‡Π½ΠΎΡΡ‚ΡŒ инсСктицидной ΠΏΡ€ΠΈΠΌΠ°Π½ΠΊΠΈ, содСрТащСй Ρ…Π»ΠΎΡ€Ρ„Π΅Π½Π°ΠΏΠΈΡ€, для Π±Π΅Π»Ρ‹Ρ… ΠΌΡ‹ΡˆΠ΅ΠΉ

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    Objective of research. The aim of this study was to estimate of acute oral toxicity for white mice and determine the hazard class of chlorfenapyr-containing baits for mammals. Materials and methods. The material of study was the insecticidal chlorfenapyr-containing bait containing the active ingredient chlorfenapyr. Experiments were carried out on laboratory animals (white mice of both gender), that after a quarantine for seven days and 24 hours of starvation were given the preparation at the dose 50-1100 mg per kg of body mass. Animals of the control group received a standard feed in the same amount. We observed animals of control and experimental groups for 10 days. According to the results of the experiment, we calculated the lethal doses (LD16, LD50, LD84)using the probit analysis As the half-lethal dose of the bait is in the range of 151-5000 mg/kg, it was referred according to gOsT 12.1.007-76 to the 3rd hazard class (moderate dangerous substances). Results and discussion. The use of the bait at the dose of 50 mg per 1 kg of body mass did not cause any toxic effects. When the dose was increased up to 100 mg per kg of body mass or more, we registered the signs of intoxication in the first 2-4 hours. We observed lethargy, muscular fibrillation, salivation. With the next 10 days no death was registered; the status of animal returned to the normal 24-48 hours after beginning of experiment. The lethal dose (LD) that led to the death of experimental animals was 1100 mg per kg of body mass. Calculated effective doses of the bait were as follows: LD16 186,0 (84,7+408,6); LD50 620,3 (355,6+1082,2), and LD84 2069,0 (941,9+4545,1) mg per kg of body mass. According to our results of acute toxicity parameters for white mice, insecticidal chlorfenapyr-containing bait belongs to the third hazard class (moderately hazardous substances).ЦСль исслСдования - ΠΎΠΏΡ€Π΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€ΠΎΠ² острой ΠΎΡ€Π°Π»ΡŒΠ½ΠΎΠΉ токсичности для Π±Π΅Π»Ρ‹Ρ… ΠΌΡ‹ΡˆΠ΅ΠΉ ΠΈ установлСниС класса опасности для Ρ‚Π΅ΠΏΠ»ΠΎΠΊΡ€ΠΎΠ²Π½Ρ‹Ρ… инсСктицидной Ρ…Π»ΠΎΡ€Ρ„Π΅Π½Π°ΠΏΠΈΡ€-содСрТащСй ΠΏΡ€ΠΈΠΌΠ°Π½ΠΊΠΈ. ΠœΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Ρ‹ ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Ρ‹. ΠœΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»ΠΎΠΌ для исслСдования слуТила инсСктицидная ΠΏΡ€ΠΈΠΌΠ°Π½ΠΊΠ°, содСрТащая Π² качСствС Π΄Π΅ΠΉΡΡ‚Π²ΡƒΡŽΡ‰Π΅Π³ΠΎ вСщСства Ρ…Π»ΠΎΡ€Ρ„Π΅Π½Π°ΠΏΠΈΡ€ (6 %). ИсслСдования ΠΏΡ€ΠΎΠ²Π΅Π΄Π΅Π½Ρ‹ Π² соотвСтствии с ΠœΠ΅Ρ‚ΠΎΠ΄ΠΈΡ‡Π΅ΡΠΊΠΈΠΌΠΈ рСкомСндациями Π½Π° Π±Π΅Π»Ρ‹Ρ… ΠΌΡ‹ΡˆΠ°Ρ… ΠΎΠ±ΠΎΠ΅Π³ΠΎ ΠΏΠΎΠ»Π°, ΠΊΠΎΡ‚ΠΎΡ€Ρ‹ΠΌ послС 7-Π΄Π½Π΅Π²Π½ΠΎΠ³ΠΎ ΠΊΠ°Ρ€Π°Π½Ρ‚ΠΈΠ½Π° ΠΈ 24-часовой Π³ΠΎΠ»ΠΎΠ΄Π½ΠΎΠΉ Π΄ΠΈΠ΅Ρ‚Ρ‹ скармливали ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚ Π² Π΄ΠΎΠ·Π°Ρ… 50-1100 ΠΌΠ³/ΠΊΠ³ массы. Π–ΠΈΠ²ΠΎΡ‚Π½Ρ‹Π΅ ΠΊΠΎΠ½Ρ‚Ρ€ΠΎΠ»ΡŒΠ½ΠΎΠΉ Π³Ρ€ΡƒΠΏΠΏΡ‹ ΠΏΠΎΠ»ΡƒΡ‡Π°Π»ΠΈ стандартный ΠΊΠΎΡ€ΠΌ Π² Ρ‚Π°ΠΊΠΎΠΌ ΠΆΠ΅ количСствС. НаблюдСниС Π·Π° состояниСм ΠΎΠΏΡ‹Ρ‚Π½Ρ‹Ρ… ΠΈ ΠΊΠΎΠ½Ρ‚Ρ€ΠΎΠ»ΡŒΠ½Ρ‹Ρ… ΠΌΡ‹ΡˆΠ΅ΠΉ Π²Π΅Π»ΠΈ Π² Ρ‚Π΅Ρ‡Π΅Π½ΠΈΠ΅ 10 суток послС скармливания ΠΏΡ€ΠΈΠΌΠ°Π½ΠΊΠΈ. По Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Π°ΠΌ ΠΎΠΏΡ‹Ρ‚ΠΎΠ² ΠΏΡ€ΠΎΠ²Π΅Π»ΠΈ расчСт Π»Π΅Ρ‚Π°Π»ΡŒΠ½ΠΎΠΉ (Π›Π”), ΠΏΠΎΠ»ΡƒΠ»Π΅Ρ‚Π°Π»ΡŒΠ½ΠΎΠΉ (Π›Π”50) ΠΈ ΠΏΡ€ΠΎΠΌΠ΅ΠΆΡƒΡ‚ΠΎΡ‡Π½Ρ‹Ρ… Π΄ΠΎΠ· ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ взвСшСнного ΠΏΡ€ΠΎΠ±ΠΈΡ‚-Π°Π½Π°Π»ΠΈΠ·Π°. Класс опасности инсСктицидной ΠΏΡ€ΠΈΠΌΠ°Π½ΠΊΠΈ устанавливали Π² соотвСтствии с Π“ΠžΠ‘Π’ 12.1.007-76. Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹ ΠΈ обсуТдСниС. Π‘ΠΊΠ°Ρ€ΠΌΠ»ΠΈΠ²Π°Π½ΠΈΠ΅ Π±Π΅Π»Ρ‹ΠΌ ΠΌΡ‹ΡˆΠ°ΠΌ Ρ…Π»ΠΎΡ€Ρ„Π΅Π½Π°ΠΏΠΈΡ€-содСрТащСй инсСктицидной ΠΏΡ€ΠΈΠΌΠ°Π½ΠΊΠΈ Π² Π΄ΠΎΠ·Π΅ 50 ΠΌΠ³/ΠΊΠ³ массы ΠΆΠΈΠ²ΠΎΡ‚Π½ΠΎΠ³ΠΎ Π½Π΅ ΡΠΎΠΏΡ€ΠΎΠ²ΠΎΠΆΠ΄Π°Π»ΠΎΡΡŒ ΠΏΡ€ΠΈΠ·Π½Π°ΠΊΠ°ΠΌΠΈ интоксикации. ΠŸΡ€ΠΈ ΡƒΠ²Π΅Π»ΠΈΡ‡Π΅Π½ΠΈΠΈ Π΄ΠΎΠ·Ρ‹ ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π° Π΄ΠΎ 100 ΠΌΠ³/ΠΊΠ³ ΠΈ Π±ΠΎΠ»Π΅Π΅ Π² ΠΏΠ΅Ρ€Π²Ρ‹Π΅ 2-4 Ρ‡ наблюдСний Ρƒ части ΠΌΡ‹ΡˆΠ΅ΠΉ фиксировали ΠΏΡ€ΠΈΠ·Π½Π°ΠΊΠΈ интоксикации. ΠšΡ€Π°Ρ‚ΠΊΠΎΠ²Ρ€Π΅ΠΌΠ΅Π½Π½ΠΎΠ΅ Π²ΠΎΠ·Π±ΡƒΠΆΠ΄Π΅Π½ΠΈΠ΅ ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ… смСнялось ΡƒΠ³Π½Π΅Ρ‚Π΅Π½Π½Ρ‹ΠΌ состояниСм; ΠΎΡ‚ΠΌΠ΅Ρ‡Π°Π»ΠΈ Π΄Ρ‹Ρ…Π°Π½ΠΈΠ΅ Π±Ρ€ΡŽΡˆΠ½ΠΎΠ³ΠΎ Ρ‚ΠΈΠΏΠ°, фибриллярноС ΠΏΠΎΠ΄Π΅Ρ€Π³ΠΈΠ²Π°Π½ΠΈΠ΅ ΠΌΡ‹ΡˆΡ†, ΡΠ»ΡŽΠ½ΠΎΠ²Ρ‹Π΄Π΅Π»Π΅Π½ΠΈΠ΅. Π’ ΠΏΠΎΡΠ»Π΅Π΄ΡƒΡŽΡ‰ΠΈΠ΅ 10 сут наблюдСний Π³ΠΈΠ±Π΅Π»ΠΈ Π½Π΅ ΠΎΡ‚ΠΌΠ΅Ρ‡Π΅Π½ΠΎ; состояниС Π²Ρ‹ΠΆΠΈΠ²ΡˆΠΈΡ… ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ… ΠΏΡ€ΠΈΡ…ΠΎΠ΄ΠΈΠ»ΠΎ ΠΊ Π½ΠΎΡ€ΠΌΠ°Π»ΡŒΠ½ΠΎΠΌΡƒ Ρ‡Π΅Ρ€Π΅Π· 24-48 Ρ‡ послС воздСйствия ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π°. Π›Π΅Ρ‚Π°Π»ΡŒΠ½Π°Ρ Π΄ΠΎΠ·Π° ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π°, Π²Ρ‹Π·Π²Π°Π²ΡˆΠ°Ρ гибСль всСх ΠΎΠΏΡ‹Ρ‚Π½Ρ‹Ρ… ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ…, составила 1100 ΠΌΠ³/ΠΊΠ³. РасчСтныС ΠΏΡ€ΠΎΠΌΠ΅ΠΆΡƒΡ‚ΠΎΡ‡Π½Ρ‹Π΅ Π΄ΠΎΠ·Ρ‹ ΠΏΡ€ΠΈΠΌΠ°Π½ΠΊΠΈ составили: Π›Π”16 186,0 (84,7-408,6), Π›Π”50 620,3 (355,6-1082,2) ΠΈ Π›Π”84 2069,0 (941,9-4545,1) ΠΌΠ³/ΠΊΠ³ массы ΠΆΠΈΠ²ΠΎΡ‚Π½ΠΎΠ³ΠΎ. ΠŸΠΎΡΠΊΠΎΠ»ΡŒΠΊΡƒ ΠΏΠΎΠ»ΡƒΠ»Π΅Ρ‚Π°Π»ΡŒΠ½Π°Ρ Π΄ΠΎΠ·Π° ΠΈΠ·ΡƒΡ‡Π΅Π½Π½ΠΎΠΉ инсСктицидной ΠΏΡ€ΠΈΠΌΠ°Π½ΠΊΠΈ укладываСтся Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½ 151-5000 ΠΌΠ³/ΠΊΠ³, Ρ‚ΠΎ ΠΏΠΎ стСпСни воздСйствия Π½Π° ΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΌ Ρ‚Π΅ΠΏΠ»ΠΎΠΊΡ€ΠΎΠ²Π½Ρ‹Ρ… ΠΎΠ½Π° относится ΠΊ 3 классу ΡƒΠΌΠ΅Ρ€Π΅Π½Π½ΠΎ опасных вСщСств

    ВыявлСниС Π² кровососущих Π΄Π²ΡƒΠΊΡ€Ρ‹Π»Ρ‹Ρ… насСкомых ВюмСнской области гСнСтичСского ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Π° Π²ΠΎΠ·Π±ΡƒΠ΄ΠΈΡ‚Π΅Π»Π΅ΠΉ вирусных Π±ΠΎΠ»Π΅Π·Π½Π΅ΠΉ ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ…

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    The purpose of the research is to test blood-sucking dipterans collected in the Tyumen Region for genetic material of viruses that cause dangerous diseases in live-stock animals.Materials and methods. From May to October 2021, blood-sucking insects of the Diptera order whose species membership was established by tabular keys were collected on pastures and farms in ten Tyumen Region districts. In 60 samples formed from the captured insects according to the taxonomic affiliation and the period and place of collection, the presence of genetic material of the leukemia provirus and the dermatitis nodularis virus of cattle (bovine) and the African swine fever virus was evaluated by polymerase chain reaction (PCR) in real time.Results and discussion. Adult insects collected for the PCR analysis were blood-sucking flies (family Muscidae, genus Stomoxys), mosquitoes (family Culicidae, genus Aedes), midges (family Simuliidae, genera Byssodon and Schoenbaueria), horseflies (family Tabanidae, genera Hybomitra, Tabanus and Haematopota), and biting midges (family Ceratopogonidae, genus Culicoides). As a result of the PCR testing of the samples for the bovine leukemia provirus DNA, 1 out of 13 samples of Stomoxys spp. (7.7%) and 1 of 13 samples of Hybomitra spp. (7.7%) were positive. The bovine leukemia provirus DNA detected in blood-sucking insects indicates the presence of this pathogen in the insect collection area as well as their possible involvement in its spread. Further research is needed on the Stomoxys spp. and Hybomitra spp. vector competence in vivo, considering natural and climatic features of the Tyumen Region.ЦСль исслСдований – тСстированиС кровососущих Π΄Π²ΡƒΠΊΡ€Ρ‹Π»Ρ‹Ρ… насСкомых, собранных Π½Π° Ρ‚Π΅Ρ€Ρ€ΠΈΡ‚ΠΎΡ€ΠΈΠΈ ВюмСнской области, Π½Π° Π½Π°Π»ΠΈΡ‡ΠΈΠ΅ гСнСтичСского ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Π° вирусов – Π²ΠΎΠ·Π±ΡƒΠ΄ΠΈΡ‚Π΅Π»Π΅ΠΉ опасных Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½Ρ‹Ρ… ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ….ΠœΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Ρ‹ ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Ρ‹. Π’ ΠΏΠ΅Ρ€ΠΈΠΎΠ΄ с мая ΠΏΠΎ ΠΎΠΊΡ‚ΡΠ±Ρ€ΡŒ 2021 Π³. Π½Π° пастбищах ΠΈ Ρ„Π΅Ρ€ΠΌΠ°Ρ… дСсяти Ρ€Π°ΠΉΠΎΠ½ΠΎΠ² ВюмСнской области Π±Ρ‹Π»ΠΈ ΠΏΡ€ΠΎΠ²Π΅Π΄Π΅Π½Ρ‹ сборы кровососущих насСкомых отряда Diptera, видовая ΠΏΡ€ΠΈΠ½Π°Π΄Π»Π΅ΠΆΠ½ΠΎΡΡ‚ΡŒ ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Ρ… Π±Ρ‹Π»Π° установлСна ΠΏΠΎ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹ΠΌ Ρ‚Π°Π±Π»ΠΈΡ†Π°ΠΌ. Π’ 60 ΠΏΡ€ΠΎΠ±Π°Ρ…, сформированных ΠΈΠ· ΠΎΡ‚Π»ΠΎΠ²Π»Π΅Π½Π½Ρ‹Ρ… насСкомых Π² соотвСтствии с таксономичСской ΠΏΡ€ΠΈΠ½Π°Π΄Π»Π΅ΠΆΠ½ΠΎΡΡ‚ΡŒΡŽ, ΠΏΠ΅Ρ€ΠΈΠΎΠ΄ΠΎΠΌ ΠΈ мСстом сбора, ΠΎΡ†Π΅Π½ΠΈΠ²Π°Π»ΠΈ Π½Π°Π»ΠΈΡ‡ΠΈΠ΅ гСнСтичСского ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Π° провируса Π»Π΅ΠΉΠΊΠΎΠ·Π° ΠΈ вируса нодулярного Π΄Π΅Ρ€ΠΌΠ°Ρ‚ΠΈΡ‚Π° ΠΊΡ€ΡƒΠΏΠ½ΠΎΠ³ΠΎ Ρ€ΠΎΠ³Π°Ρ‚ΠΎΠ³ΠΎ скота (КРБ), вируса африканской Ρ‡ΡƒΠΌΡ‹ свинСй ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠ»ΠΈΠΌΠ΅Ρ€Π°Π·Π½ΠΎΠΉ Ρ†Π΅ΠΏΠ½ΠΎΠΉ Ρ€Π΅Π°ΠΊΡ†ΠΈΠΈ (ПЦР) Π² Ρ€Π΅ΠΆΠΈΠΌΠ΅ Ρ€Π΅Π°Π»ΡŒΠ½ΠΎΠ³ΠΎ Π²Ρ€Π΅ΠΌΠ΅Π½ΠΈ.Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹ ΠΈ обсуТдСниС. Π‘ΠΎΠ±Ρ€Π°Π½Π½Ρ‹Π΅ для ПЦР-исслСдования взрослыС особи насСкомых Π±Ρ‹Π»ΠΈ прСдставлСны кровососущими ΠΌΡƒΡ…Π°ΠΌΠΈ (сСм. Muscidae, Ρ€ΠΎΠ΄ Stomoxys), ΠΊΠΎΠΌΠ°Ρ€Π°ΠΌΠΈ (сСм. Culicidae, Ρ€ΠΎΠ΄ Aedes), мошками (сСм. Simuliidae, Ρ€ΠΎΠ΄Ρ‹ Byssodon ΠΈ Schoenbaueria), слСпнями (сСм. Tabanidae, Ρ€ΠΎΠ΄Ρ‹ Hybomitra, Tabanus ΠΈ Haematopota), ΠΌΠΎΠΊΡ€Π΅Ρ†Π°ΠΌΠΈ (сСм. Ceratopogonidae, Ρ€ΠΎΠ΄ Culicoides). Π’ Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Π΅ ПЦР-тСстирования ΠΏΡ€ΠΎΠ± Π½Π° Π½Π°Π»ΠΈΡ‡ΠΈΠ΅ Π”ΠΠš провируса Π»Π΅ΠΉΠΊΠΎΠ·Π° КРБ ΠΏΠΎΠ»ΠΎΠΆΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹ΠΌΠΈ оказались 1 ΠΈΠ· 13 ΠΏΡ€ΠΎΠ± Stomoxys spp. (7,7%) ΠΈ 1 ΠΈΠ· 13 ΠΏΡ€ΠΎΠ± Hybomitra spp. (7,7%). ВыявлСниС Π”ΠΠš провируса Π»Π΅ΠΉΠΊΠΎΠ·Π° КРБ Π² кровососущих насСкомых ΡƒΠΊΠ°Π·Ρ‹Π²Π°Π΅Ρ‚ Π½Π° присутствиС Π΄Π°Π½Π½ΠΎΠ³ΠΎ возбудитСля Π½Π° Ρ‚Π΅Ρ€Ρ€ΠΈΡ‚ΠΎΡ€ΠΈΠΈ сбора насСкомых, Π° Ρ‚Π°ΠΊΠΆΠ΅ Π½Π° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠ΅ ΠΈΡ… участиС Π² Π΅Π³ΠΎ распространСнии. НСобходимы дальнСйшиС исслСдования Π²Π΅ΠΊΡ‚ΠΎΡ€Π½ΠΎΠΉ компСтСнтности Stomoxys spp. ΠΈ Hybomitra spp. Π² СстСствСнных условиях с ΡƒΡ‡Π΅Ρ‚ΠΎΠΌ ΠΏΡ€ΠΈΡ€ΠΎΠ΄Π½ΠΎ-климатичСских особСнностСй ВюмСнской области

    Stomoxys calcitrans (Diptera: muscidae): means and methods of control. Overview

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    The purpose of the research: to analyze and summarize literature data on the main means and methods of protecting animals from stable flies Stomoxys calcitrans and to reduce their number.Materials and methods. The analysis of literature data from foreign sources over the past 30 years describing the means and methods of controlling the numbers of S. calcitrans and ways to protect animals from their attack. Results and discussion. A literature review on the means and methods of controlling the abundance of S. calcitrans is presented. Chemicals are widespread and can be used to combat the pre-adult stages of development of flies by introducing them into the substrate or feeding animals, as well as to combat adult insects by treating surfaces or in traps. In the description of physical control methods, attention is paid to traps and various methods of attracting insects to them. As biological control methods for S. calcitrans, researchers are actively studying the possibilities and prospects of using parasitoids (for example, Hymenoptera, including four families and about ten species), more than ten species of ticks from different families, entomopathogenic nematodes (a species of Heterorhabditis bacteriophora), fungi and individual species bacteria

    <i>Stomoxys calcitrans</i> (Diptera: Muscidae): Value for Veterinary Medicine. Review

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    The purpose of the research is analysis and compilation of literature data on involvement of the stable fly Stomoxys calcitrans in the spread of livestock animal pathogens. Materials and methods. We have analyzed literature data from foreign sources over the past 30 years, which are indexed in the PubMed, Crossref, Web of Science and Scopus databases, about S. calcitrans as a potential vector of livestock animal pathogens. Results and discussion. A literature review is presented on assessment of economic impact by the stable fly S. calcitrans on animal husbandry, as well as possible involvement of the S. calcitrans imago in the transmission of viruses, bacteria, protozoa and helminths which are animal pathogens

    Acute toxicity of insecticidal chlorfenapyr-containing bait for mice

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    Objective of research. The aim of this study was to estimate of acute oral toxicity for white mice and determine the hazard class of chlorfenapyr-containing baits for mammals. Materials and methods. The material of study was the insecticidal chlorfenapyr-containing bait containing the active ingredient chlorfenapyr. Experiments were carried out on laboratory animals (white mice of both gender), that after a quarantine for seven days and 24 hours of starvation were given the preparation at the dose 50-1100 mg per kg of body mass. Animals of the control group received a standard feed in the same amount. We observed animals of control and experimental groups for 10 days. According to the results of the experiment, we calculated the lethal doses (LD16, LD50, LD84)using the probit analysis As the half-lethal dose of the bait is in the range of 151-5000 mg/kg, it was referred according to gOsT 12.1.007-76 to the 3rd hazard class (moderate dangerous substances). Results and discussion. The use of the bait at the dose of 50 mg per 1 kg of body mass did not cause any toxic effects. When the dose was increased up to 100 mg per kg of body mass or more, we registered the signs of intoxication in the first 2-4 hours. We observed lethargy, muscular fibrillation, salivation. With the next 10 days no death was registered; the status of animal returned to the normal 24-48 hours after beginning of experiment. The lethal dose (LD) that led to the death of experimental animals was 1100 mg per kg of body mass. Calculated effective doses of the bait were as follows: LD16 186,0 (84,7+408,6); LD50 620,3 (355,6+1082,2), and LD84 2069,0 (941,9+4545,1) mg per kg of body mass. According to our results of acute toxicity parameters for white mice, insecticidal chlorfenapyr-containing bait belongs to the third hazard class (moderately hazardous substances)

    Stomoxys calcitrans (Diptera: Muscidae): Π²Π΅Ρ‚Π΅Ρ€ΠΈΠ½Π°Ρ€Π½ΠΎΠ΅ Π·Π½Π°Ρ‡Π΅Π½ΠΈΠ΅. ΠžΠ±Π·ΠΎΡ€

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    The purpose of the research is analysis and compilation of literature data on involvement of the stable fly Stomoxys calcitrans in the spread of livestock animal pathogens. Materials and methods. We have analyzed literature data from foreign sources over the past 30 years, which are indexed in the PubMed, Crossref, Web of Science and Scopus databases, about S. calcitrans as a potential vector of livestock animal pathogens. Results and discussion. A literature review is presented on assessment of economic impact by the stable fly S. calcitrans on animal husbandry, as well as possible involvement of the S. calcitrans imago in the transmission of viruses, bacteria, protozoa and helminths which are animal pathogens.ЦСль исслСдований: Π°Π½Π°Π»ΠΈΠ· ΠΈ ΠΎΠ±ΠΎΠ±Ρ‰Π΅Π½ΠΈΠ΅ Π»ΠΈΡ‚Π΅Ρ€Π°Ρ‚ΡƒΡ€Π½Ρ‹Ρ… Π΄Π°Π½Π½Ρ‹Ρ… ΠΎΠ± участии осСннСй ΠΆΠΈΠ³Π°Π»ΠΊΠΈ Stomoxys calcitrans Π² распространСнии Π²ΠΎΠ·Π±ΡƒΠ΄ΠΈΡ‚Π΅Π»Π΅ΠΉ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½Ρ‹Ρ… ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ…. ΠœΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Ρ‹ ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Ρ‹. ΠŸΡ€ΠΎΠ²Π΅Π΄Π΅Π½ Π°Π½Π°Π»ΠΈΠ· Π»ΠΈΡ‚Π΅Ρ€Π°Ρ‚ΡƒΡ€Π½Ρ‹Ρ… Π΄Π°Π½Π½Ρ‹Ρ… ΠΈΠ· Π·Π°Ρ€ΡƒΠ±Π΅ΠΆΠ½Ρ‹Ρ… источников Π·Π° послСдниС 30 Π»Π΅Ρ‚, проиндСксированных Π² Π±Π°Π·Π°Ρ… PubMed, Crossref, Web of Science ΠΈ Scopus, ΠΎ S. calcitrans ΠΊΠ°ΠΊ ΠΏΠΎΡ‚Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½ΠΎΠΌ пСрСносчикС Π²ΠΎΠ·Π±ΡƒΠ΄ΠΈΡ‚Π΅Π»Π΅ΠΉ Π±ΠΎΠ»Π΅Π·Π½Π΅ΠΉ ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½Ρ‹Ρ… ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ…. Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹ ΠΈ обсуТдСниС. ΠŸΡ€ΠΈΠ²Π΅Π΄Π΅Π½ Π»ΠΈΡ‚Π΅Ρ€Π°Ρ‚ΡƒΡ€Π½Ρ‹ΠΉ ΠΎΠ±Π·ΠΎΡ€ ΠΏΠΎ ΠΎΡ†Π΅Π½ΠΊΠ΅ экономичСского влияния осСннСй ΠΆΠΈΠ³Π°Π»ΠΊΠΈ S. calcitrans Π½Π° Тивотноводство, Π° Ρ‚Π°ΠΊΠΆΠ΅ ΠΎ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠΌ участии ΠΈΠΌΠ°Π³ΠΎ S. calcitrans Π² ΠΏΠ΅Ρ€Π΅Π΄Π°Ρ‡Π΅ вирусов, Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠΉ, ΠΏΡ€ΠΎΡΡ‚Π΅ΠΉΡˆΠΈΡ… ΠΈ Π³Π΅Π»ΡŒΠΌΠΈΠ½Ρ‚ΠΎΠ², ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ ΡΠ²Π»ΡΡŽΡ‚ΡΡ возбудитСлями Π±ΠΎΠ»Π΅Π·Π½Π΅ΠΉ ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ…

    Epizootology anaplasmosis and babesiosis the reindeer in the Tyumen region

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    РассмотрСна эпизоотичСская ситуация ΠΏΠΎ Π°Π½Π°ΠΏΠ»Π°Π·ΠΌΠΎΠ·Ρƒ ΠΈ Π±Π°Π±Π΅Π·ΠΈΠΎΠ·Ρƒ сСвСрного олСня Π² хозяйствах Ямальского ΠΈ Вазовского Ρ€Π°ΠΉΠΎΠ½ΠΎΠ² Π―ΠΌΠ°Π»ΠΎ-НСнСцкого Π°Π²Ρ‚ΠΎΠ½ΠΎΠΌΠ½ΠΎΠ³ΠΎ ΠΎΠΊΡ€ΡƒΠ³Π°. ΠžΠ±Π½Π°Ρ€ΡƒΠΆΠ΅Π½Π° ΠΈΠ½Π²Π°Π·ΠΈΡ€ΠΎΠ²Π°Π½Π½ΠΎΡΡ‚ΡŒ сСвСрных ΠΎΠ»Π΅Π½Π΅ΠΉ Anaplasma marginale ΠΈ Babesia spp. Π² Ρ„ΠΎΡ€ΠΌΠ΅ ΠΏΠ°Ρ€Π°Π·ΠΈΡ‚ΠΎΠ½ΠΎΡΠΈΡ‚Π΅Π»ΡŒΡΡ‚Π²Π°. Π£ обслСдованных ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ… ΠΎΡ‚ΠΌΠ΅Ρ‡Π΅Π½ΠΎ сСзонноС сниТСниС гСматологичСских ΠΏΠΎΠΊΠ°Π·Π°Ρ‚Π΅Π»Π΅ΠΉ.We surveyed the epizootolgy situation on anaplasmosis and babesiosis of reindeer in Yamal-Nenets autonomous region. The form of found Anaplasma marginale and Babesia spp. reindeer’s infection is a parasite carrying. We found the seasonal decrease of blood parameters of reindeers

    Insecticidal impact of the mixture of fipronil and fir oil on adult Musca domestica

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    Π’ настоящСС врСмя Π² Π²Π΅Ρ‚Π΅Ρ€ΠΈΠ½Π°Ρ€Π½ΠΎΠΉ дСзинсСкции Π°ΠΊΡ‚ΡƒΠ°Π»ΡŒΠ½Ρ‹ исслСдования ΠΏΠΎ Ρ€Π°Π·Ρ€Π°Π±ΠΎΡ‚ΠΊΠ΅ эффСктивных ΠΈ простых Π² ΠΏΡ€ΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠΈ инсСктицидов. ВозрастаСт ΠΏΠΎΡ‚Ρ€Π΅Π±Π½ΠΎΡΡ‚ΡŒ Π² соврСмСнных инсСктицидных срСдствах с минимальной экологичСской ΠΎΠΏΠ°ΡΠ½ΠΎΡΡ‚ΡŒΡŽ. Π’Π°ΠΊΠΈΠΌΠΈ ΡΠ²Π»ΡΡŽΡ‚ΡΡ, Π½Π°ΠΏΡ€ΠΈΠΌΠ΅Ρ€, ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Ρ‹ Π½Π° основС Ρ€Π°ΡΡ‚ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠ³ΠΎ ΡΡ‹Ρ€ΡŒΡ.Π’ Π΄Π°Π½Π½ΠΎΠΉ Ρ€Π°Π±ΠΎΡ‚Π΅ ΠΈΠ·ΡƒΡ‡Π΅Π½ΠΎ инсСктицидноС дСйствиС Π½Π° ΠΈΠΌΠ°Π³ΠΎ Musca domestica смСси Ρ„ΠΈΠΏΡ€ΠΎΠ½ΠΈΠ»Π° ΠΈ масла ΠΏΠΈΡ…Ρ‚Ρ‹, Π° Ρ‚Π°ΠΊΠΆΠ΅ ΠΎΠΏΡ€Π΅Π΄Π΅Π»Π΅Π½Π° Π΅Π΅ ΡΡ‚Π°Π±ΠΈΠ»ΡŒΠ½ΠΎΡΡ‚ΡŒ ΠΈ ΠΊΡ€ΠΈΠΎΡΡ‚ΠΎΠΉΠΊΠΎΡΡ‚ΡŒ. ΠŸΡ€ΠΎΡ‚Π΅ΡΡ‚ΠΈΡ€ΠΎΠ²Π°Π½Ρ‹ смСси с ΡΠΎΠΎΡ‚Π½ΠΎΡˆΠ΅Π½ΠΈΠ΅ΠΌ ΡƒΠΊΠ°Π·Π°Π½Π½Ρ‹Ρ… ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½Ρ‚ΠΎΠ² 1:225, 1:450, 1:900 ΠΈ 1:1800, Π²ΠΎ всСх случаях Ρ…Π°Ρ€Π°ΠΊΡ‚Π΅Ρ€ совмСстногдСйствия Ρ„ΠΈΠΏΡ€ΠΎΠ½ΠΈΠ»Π° ΠΈ масла ΠΏΠΈΡ…Ρ‚Ρ‹ Π°Π΄Π΄ΠΈΡ‚ΠΈΠ²Π½Ρ‹ΠΉ. НаибольшСС Π·Π½Π°Ρ‡Π΅Π½ΠΈΠ΅ коэффициСнта совмСстного дСйствия ΠΎΡ‚ΠΌΠ΅Ρ‡Π΅Π½ΠΎ для ΡΠΎΠΎΡ‚Π½ΠΎΡˆΠ΅Π½ΠΈΡ 1:450, смСсь с Π΄Π°Π½Π½Ρ‹ΠΌ ΡΠΎΠΎΡ‚Π½ΠΎΡˆΠ΅Π½ΠΈΠ΅ΠΌ Ρ„ΠΈΠΏΡ€ΠΎΠ½ΠΈΠ»Π° ΠΈ масла ΠΏΠΈΡ…Ρ‚Ρ‹ ΠΏΠΎ инсСктицидному Π΄Π΅ΠΉΡΡ‚Π²ΠΈΡŽ ΠΏΡ€ΠΎΡ‚ΠΈΠΈΠΌΠ°Π³ΠΎ ΠΌΡƒΡ… относится ΠΊ срСднСэффСктивным, Π±Ρ‹ΡΡ‚Ρ€ΠΎΠ΄Π΅ΠΉΡΡ‚Π²ΡƒΡŽΡ‰ΠΈΠΌ с нСобратимыинсСктицидным эффСктом. ΠžΠΏΡ€Π΅Π΄Π΅Π»Π΅Π½Ρ‹ ΡΠΌΠ΅Ρ€Ρ‚Π΅Π»ΡŒΠ½Ρ‹Π΅ Π΄ΠΎΠ·Ρ‹ Π‘Π”50, Π‘Π”84 ΠΈ Π‘Π”99 смСс(1:450) для ΠΌΡƒΡ… Π² зависимости ΠΎΡ‚ Ρ‚ΠΈΠΏΠ° ΠΎΠ±Ρ€Π°Π±Π°Ρ‚Ρ‹Π²Π°Π΅ΠΌΠΎΠΉ повСрхности. УстановлСна острая Ρ‚ΠΎΠΊΡΠΈΡ‡Π½ΠΎΡΡ‚ΡŒ ΡƒΠΊΠ°Π·Π°Π½Π½ΠΎΠΉ смСси для Π±Π΅Π»Ρ‹Ρ… ΠΌΡ‹ΡˆΠ΅ΠΉ (II класс опасности Π² соотвСтствии с Π“ΠžΠ‘Π’ 12.1.007-76).At present veterinary pest control studies focus on development of effective and simple insecticides. There is a growing demand for modern environmentally friendly insecticides; for example those made of plant-based raw materials. In this research we have studied the insecticidal effect of the mixture of fipronil and fir oil on adult species of Musca domestica as well as its permanence and cryostability. Mixtures of fipronil and fir oil in ratios 1:225, 1:450, 1:900 and 1:1800 have been tested. In all cases, the nature of the component interaction was additive. The greatest value of the combination index was found in 1:450 ratio. This mixture used against adult flies is classified as of average effeciency, fast-acting with an irreversible insecticidal effect. Lethal doses LD50, LD84 and LD99 of the mixture (1:450) for flies have been determined according to the type of the treated surface. Acute toxicity of the mixture for mice (hazard class II in accordance with GOST 12.1.007-76) has been determined
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