THE SYSTEM OF PHARMACOVIGILANCE AT A PHARMACEUTICAL PLANT

The article presents information about the organization of pharmacovigilance system in a pharmaceutical company. The main tasks and methods of pharmacovigilance achievement are described. The system of notification of adverse reactions of pharmacotherapy is described

RESULTS OF PRECLINICAL SAFETY TESTING COMPARISON FOR IBUPROFEN DRUG PRODUCTS

The article contains materials, methods and results of preclinical experimental test of acute toxicity of drugs «Ibuprofen», suspension for oral administration [for children], LP 001651 from 13.04.2012 and referent «Nurofen for children», suspension for oral administration, P N014745/01 from 22.08.2008. Ibuprofen preparations compared were found equivalent in acute toxicity studies. The work will be continued in order to prove the bioequivalence of the claimed preparations

EVALUATION OF CLINICAL AND FUNCTIONAL OUTCOME OF MULTIFOCAL CORRECTION BY CONTACT LENS IN PATIENTS WITH PRESBYOPIA AND MYOPIA REFRACTION

The aim of the study was to evaluate the effectiveness of multifocal contact lens correction in patients with presbyopia and myopic refractive error. The study involved 45 patients with myopia ranging in age from 43 to 56 years in conditions of habitual monofocal contact lens correction, a month after the selection and wearing multifocal contact lenses. Contact correction was carried out according to standard procedures taking into account the accuracy of contact lens fitting, with carrying out functional tests for the "dry eye" syndrome and evaluation of corneal epithelial thickness (Optovue, USA). The assessment of the following parameters of visual perception was made: far, near and middle distance (monocular and binocular) visual acuity, the spatial contrast sensitivity, stereovision (I & II Lang test), the area of binocular interaction field (binarymeter), the quality of vision (VF-14 test ). The use of multifocal contact lenses for the correction of presbyopia with the initial myopic refraction ensures high functional results at different distances. Patients with a myopic refraction, previously used contact lenses, easy pass to multifocal correction option. The greatest satisfaction by achieved vision noted in those patients who had achieved refraction (-)0.5 diopters. In the selection of contact lenses in older patients the thickness of the corneal epithelium and tear film validity must be taken into account

Advantages and disadvantages of multifocal contact lens correction of presbyopia in patients with myopic refraction

Purpose: To evaluate the results of multifocal contact correction of presbyopia in subjects with myopic refraction.Methods: The study included 28 patients with myopia (range 43 to 54 years) in conditions of routine contact correction of vision. The examination was repeated a month later after wearing of multifocal contact lenses. The following parameters were assessed: far visual acuity, near and intermediate visual acuity (monocular and binocular), spatial contrast sensitivity, stereo vision (Lang test I&II), area of binocular interaction (binarymeter), visual quality (test VF-14).Results: The indices if visual acuity in patients with multifocal contact lenses were high at all test distances. The significant increase of near vision was noted: visual acuity with monofocal contact correction was 0.39±0.11, with multifocal — 0.62±0.15 (p<0.05). However the decrease of spatial contrast sensitivity was noted: with monofocal contact lenses 21.96±4.6 dB (total in 4 frequencies), with multifocal — 19.48±1.1 dB, p<0.05. The level of stereo perception in users of multifocal contact lenses remains high, but the decrease of fusion capacity in convergence was noted. The greatest satisfaction with obtained vision was noted by patients, who achieved refraction (-) 0.5 D or difference between eyes 0.3‑0.5 D. The office employees were maximally satisfied with multifocal contact lenses.Conclusion: Multifocal contact correction of presbyopia allows achieving high functional results at different distances. Patients with myopia, previously used contact lens correction, easy pass to multifocal correction options. The greatest satisfaction with obtained vision was noted by patients, who achieved refraction (-) 0.5 D

19 F electron nuclear double resonance (ENDOR) spectroscopy for distance measurements using trityl spin labels in DNA duplexes

The combination of fluorine labeling and pulsed electron-nuclear double resonance (ENDOR) is emerging as a powerful technique for obtaining structural information about proteins and nucleic acids.The combination of fluorine labeling and pulsed electron-nuclear double resonance (ENDOR) is emerging as a powerful technique for obtaining structural information about proteins and nucleic acids. In this work, we explored the capability of Mims 19 F ENDOR experiments on reporting intermolecular distances in trityl- and 19 F-labeled DNA duplexes at three electron paramagnetic resonance (EPR) frequencies (34, 94, and 263 GHz). For spin labeling, we used the hydrophobic Finland trityl radical and hydrophilic OX063 trityl radical. Fluorine labels were introduced into two positions of a DNA oligonucleotide. The results indicated that hyperfine splittings visible in the ENDOR spectra are consistent with the most populated interspin distances between 19 F and the trityl radical predicted from molecular dynamic (MD) simulations. Moreover, for some cases, ENDOR spectral simulations based on MD results were able to reproduce the conformational distribution reflected in the experimental ENDOR line broadening. Additionally, MD simulations provided more detailed information about the melting of terminal base pairs of the oligonucleotides and about the configuration of the trityls relative to a DNA end.Russian Science Foundation https://doi.org/10.13039/50110000676