Human phospholipases A2: a functional and evolutionary analysis

Phospholipases A2 (PLA2) are capable of hydrolyzing the sn-2 position of glycerophospholipids to release fatty acids and lysophospholipids. The PLA2 superfamily enzymes are widespread and present in most mammalian cells and tissues, regulating metabolism, remodeling the membrane and maintaining its homeostasis, producing lipid mediators and activating inflammatory reactions, so disruption of PLA2-regulated lipid metabolism often leads to various diseases. In this study, 29 PLA2 genes in the human genome were systematically collected and described based on literature and sequence analyses. Localization of the PLA2 genes in human genome showed they are placed on 12 human chromosomes, some of them forming clusters. Their RVI scores estimating gene tolerance to the mutations that accumulate in the human population demonstrated that the G4-type PLA2 genes belonging to one of the two largest clusters (4 genes) were most tolerant. On the contrary, the genes encoding G6-type PLA2s (G6B, G6F, G6C, G6A) localized outside the clusters had a reduced tolerance to mutations. Analysis of the association between PLA2 genes and human diseases found in the literature showed 24 such genes were associated with 119 diseases belonging to 18 groups, so in total 229 disease/PLA2 gene relationships were described to reveal that G4, G2 and G7-type PLA2 proteins were involved in the largest number of diseases if compared to other PLA2 types. Three groups of diseases turned out to be associated with the greatest number of PLA2 types: neoplasms, circulatory and endocrine system diseases. Phylogenetic analysis showed that a common origin can be established only for secretory PLA2s (G1, G2, G3, G5, G10 and G12). The remaining PLA2 types (G4, G6, G7, G8, G15 and G16) could be considered evolutionarily independent. Our study has found that the genes most tolerant to PLA2 mutations in humans (G4, G2, and G7 types) belong to the largest number of disease groups

Model of demand for labor in the urfo pharmaceutical market

The purpose of the study is to comprehensive assessment of the structure of demand for labor in the pharmaceutical market of the URFO to build empirical functions of the demand for labor resources for pharmaceutical specialists with different qualifications.Цель исследования – комплексная оценка структуры спроса на труд на фармацевтическом рынке УрФО для построения эмпирических функций спроса на трудовые ресурсы для фармацевтических специалистов с разной квалификацией

Special aspects of communication in a multi-religious space of modern society

The article is devoted to the peculiarities of communication in modern society. The following spheres of a religious discourse functioning are defined: communication in church, communication in small religious groups, communication of the person with God. Besides, each religion has its system of values. The system of values of each religion is not a stable phenomenon and can change with the change of society. Communication in a multi-religious society has some peculiarities. In order to overcome difficulties during communication in multi-religious space of modern society, it is important to know and consider the values of people professing this religion

STUDY OF POSSIBILITY OF APPLICATION OF COLD-HARDENING MIXTURES WITH DIFFERENT GUMS IN CONDITIONS OF FGUP “URALVAGONZAVOD

The possibilities of application of cold-hardening mixtures with different gums in conditions of Uralvagonzavod are examined

Study of baicalin hydrolysis kinetics in the process of its extraction from Scutellaria baicalensis Georgi roots

The aim of this study was to investigate the kinetics of baicalin hydrolysis in the process of its extraction from Scutellaria baicalensis Georgi roots. Materials and methods. For the studies, Scutellaria baicalensis Georgi roots with a particle range of 0.1.0.5 mm were used. The method of extraction was a simple maceration during a specified period of time, the ratio of plant raw material : extractant was 1:10 w/v at the temperature of 24±1°C. Baicalin and baicalein contents were analyzed by reverse phase high performance liquid chromatography (RP HPLC) at the analytical wavelength of 275 nm. The extractant was a water solution of ethanol 26, 43, 59, 72, 81, 97±1% v/v. The time of the extraction was from 1 to 24 hours. Results. The experimental points of dependency of baicalin concentration in the extract on the time of extraction for ethanol solutions with a concentration of 43 and 72% v/v are closely approximated by a linear equation in coordinates lnC=f(t). The value of determination coefficient is more than R2>0,99. Half lifetime for baicalin has been calculated: for ethanol with the concentration of 43% v/v it is 4.3±0.7 hours, and for ethanol with the concentration of 72% v/v it is 42.3±1.8 hours. Conclusion. Baicalin hydrolysis kinetics in the process of its extraction from Scutellaria baicalensis Georgi roots with 43 and 72% v/v ethanol concentration. has been studied. It has been established that the process of baicalin hydrolysis is well described by the first order kinetic equation. The constants of baicalin hydrolysis during its extraction from Scutelaria baicalensis roots with ethanol having different concentrations have been calculated. Recommendations on technology optimization for baicalin or baicalein extraction from Scutellaria baicalensis Georgi roots have been given. © 2019 Volgograd State Medical University, Pyatigorsk Medical and Pharmaceutical Institute. All rights reserved

TO THE 95TH ANNIVERSARY OF THE DEPARTMENT OF PROPEDEUTICS OF INTERNAL DISEASES

Aim. To show the significance of the department of propedeutics of internal diseases in the structure of higher medical education and its role in the education of medical personnel in Kuban Medical University since its establishment. The article is devoted to the work of one of the oldest departments of Kuban State Medical University − department of propedeutics of internal diseases, − which was founded in 1922. The discipline taught in the department is one of the key stages of internal diseases studying. During the existence of the department, such prominent figures of medical science of the Kuban worked there: N.N. Niyibizi, K.M. Rutkevich, P.I. Budarin, K.A. Patsevich. The historical path of the department in many ways reflects the history of the University, the region and the country.Conclusion. The staff of the department has made a significant contribution to the development of healthcare of Krasnodar region and continues work on the training of doctors

Rezul'taty primeneniya preduktala (trimetazidina) u pozhilykh patsientov s refrakternoy stenokardiey, stradayushchikh sakharnym diabetom

Представлены результаты клинического исследования по оценки эффективности добавления триметазидина к монотерапии изосорбида динитратом у пациентов с сахарным диабетом. Указанная терапия позволяла эффективно устранять ишемию миокарда, рефрактерную по отношению к нитропрепаратам. Препарат активно влияет на энергетический метаболизм ишемизированного миокарда, причем - наиболее эффективен в условиях нарушенного углеводного обмена. Отсутствие какой-либо гемодинамической активности значительно улучшает профиль переносимости триметазидина и позволяет считать его идеальным препаратом выбора для лечения пожилых пациентов, предрасположенных к развитию побочных эффектов на фоне традиционной антиангинальной терапии. Метаболический механизм действия, реализующийся на клеточном уровне и не связанный с изменениями гемодинамики, определяет абсолютную аддитивность триметазидина по отношению к традиционным антиангинальным препаратам. Исследование продемонстрировало, что триметазидин можно рекомендовать, в качестве полноценной альтернативы современным методам реваскуляризации миокарда у больных ИБС с такими неблагоприят ными особенностями течения заболевания, как пожилой возраст, наличие СД и рефрактерность стенокардии по отношению к традиционным антиангинальным препаратам

Analysis of the determinants of labor demand in the pharmaceutical market segment UrFO

The aim of the study – to analysis of the territorial demand for pharmaceutical human resources in the URFO for the qualitative and quantitative characteristics of the need for pharmaceutical competencies in the labor market in pharmacy.Цель исследования – анализ территориального спроса на фармацевтические кадровые ресурсы в УрФО для качественной и количественной характеристики потребности в фармацевтических компетенциях на рынке труда в фармации