An investigation into the impact of human age on persuasion and advertising.

The elderly segment of this country\u27s population is growing rapidly. By the beginning of the next century, adults age 65 and over will fully comprise one-quarter of the population. It cannot be assumed that communication principles that apply to young adults are directly applicable to elderly adults. Age-related changes in learning, memory and information processing can have a dramatic effect on communicating with an aging population. The Elaboration Likelihood Model developed by Petty and Cacioppo provided the underlying persuasion theory for this study. Previous advertising and gerontological research provided additional theoretical basis for the research hypotheses. This study examined the impact of personal relevance and type of ad copy on the persuasion process for elderly as compared to young adults. More specifically, the research objectives were: (1) To examine information processing differences for persuasive communications between elderly and young adults under conditions of high and low personal relevance. (2) To study the persuasiveness and memorability of abstract and concrete ad copy for elderly and young adults. (3) To identify reasons for age-related deficits in memory. To accomplish these objectives, chronological age, personal relevance and type of ad copy factors were manipulated in a 2 x 2 x 2 between subjects design. There were two levels each of age (young/old adults), personal relevance (high/low), and type of ad copy (abstract/concrete). Two hundred, thirty-eight young and elderly subjects were studied. All subjects participated in an experimental session in which they examined a sample magazine containing stimulus advertisements and then completed a questionnaire. Results of the study showed that young adults appear to have better aided recall and recognition memory for advertisements than older adults. There were differential effects for abstract and concrete ad copy. For both age groups, concrete was remembered better than abstract ad copy. Yet, elderly adults exposed to concrete ad copy appeared to have higher levels of purchase intent in the high personal relevance condition than in the low personal relevance condition. Overall, elderly adults had higher levels of purchase intent than young adults, but poorer memory performance. Finally, memory deficits among elderly adults appeared to stem from problems recalling information from memory, not due to encoding information into memory. The implications of the study findings for advertising are many. These relate to the type of copy used in print advertisements, media selection for reaching the elderly adult, and advertisement effectiveness measurement, to name a few. It is clear that advertisers need to approach elderly adults differently than they do young adults. Future research directions are presented

Serologic response to culture filtrate antigens of Mycobacterium ulcerans during Buruli ulcer disease.

Buruli ulcer (BU) is an emerging necrotic skin disease caused by Mycobacterium ulcerans. To assess the potential for a serodiagnostic test, we measured the humoral immune response of BU patients to M. ulcerans antigens and compared this response with delayed-type hypersensitivity responses to both Burulin and PPD. The delayed-type hypersensitivity response generally supported the diagnosis of BU, with overall reactivity to Burulin in 28 (71.8%) of 39 patients tested, compared with 3 (14%) of 21 healthy controls. However, this positive skin test response was observed primarily in patients with healed or active disease, and rarely in patients with early disease (p=0.009). When tested for a serologic response to M. ulcerans culture filtrate, 43 (70.5%) of 61 BU patients had antibodies to these antigens, compared with 10 (37.0%) of 27 controls and 4 (30. 8%) of 13 tuberculosis patients. There was no correlation between disease stage and the onset of this serum antibody response. Our findings suggest that serologic testing may be useful in the diagnosis and surveillance of BU

Effect of high-pressure hot-water washing treatment on fruit quality, insects, and disease in apples and pears Part III. Use of silicone-based materials and mechanical methods to eliminate surface pests

Surface arthropods on pome fruits can cause export problems and disrupt commercial markets. Eliminating insects and mites on the packing line would be the last opportunity to provide for pest-free produce. In this study, an experimental packing line was used to evaluate techniques using different surfactant baths, pressurized water sprays, and styles of rotating brushes to remove field-collected and laboratory-reared grape mealybug, Pseudococcus maritimus (Ehrhorn) (Homoptera: Pseudococcidae), the diapausing two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) and the woolly apple aphid, Eriosoma lanigerum (Hausman) (Homoptera: Aphididae). The organosilicone Silwet L-77 was no more effective than a silicone-based food grade defoamer in aiding removal. Mechanical methods, such as the style of rotating brushes and pressurized sprays, were significantly effective in removing surface arthropods. No improvement in removal occurred when pressure was increased beyond 420 kPa. These techniques can be easily adapted to commercial facilities and will reduce the incidence of surface arthropods on marketed fresh fruits

Rapid Progressing Allele HLA-B35 Px Restricted Anti-HIV-1 CD8+ T Cells Recognize Vestigial CTL Epitopes

BACKGROUND: The HLA-B*35-Px allele has been associated with rapid disease progression in HIV-1 infection, in contrast to the HLA-B*35-Py allele. METHODOLOGY/PRINCIPAL FINDINGS: Immune responses to two HLA-B*35 restricted HIV-1 specific CTL epitopes and their variants were followed longitudinally during early HIV-1 infection in 16 HLA-B*35+ individuals. Subjects expressing HLA-B*35-Px alleles showed no difference in response to the consensus epitopes compared to individuals with HLA-B*35-Py alleles. Surprisingly, all the HLA-B*35-Px+ individuals responded to epitope-variants even in the absence of a consensus response. Sequencing of the viral population revealed no evidence of variant virus in any of the individuals. CONCLUSIONS/SIGNIFICANCE: This demonstrates a novel phenomenon that distinguishes individuals with the HLA-B*35-Px rapid progressing allele and those with the HLA-B*35-Py slower progressing allele

T Cell Responses to Human Endogenous Retroviruses in HIV-1 Infection

Human endogenous retroviruses (HERVs) are remnants of ancient infectious agents that have integrated into the human genome. Under normal circumstances, HERVs are functionally defective or controlled by host factors. In HIV-1-infected individuals, intracellular defense mechanisms are compromised. We hypothesized that HIV-1 infection would remove or alter controls on HERV activity. Expression of HERV could potentially stimulate a T cell response to HERV antigens, and in regions of HIV-1/HERV similarity, these T cells could be cross-reactive. We determined that the levels of HERV production in HIV-1-positive individuals exceed those of HIV-1-negative controls. To investigate the impact of HERV activity on specific immunity, we examined T cell responses to HERV peptides in 29 HIV-1-positive and 13 HIV-1-negative study participants. We report T cell responses to peptides derived from regions of HERV detected by ELISPOT analysis in the HIV-1-positive study participants. We show an inverse correlation between anti-HERV T cell responses and HIV-1 plasma viral load. In HIV-1-positive individuals, we demonstrate that HERV-specific T cells are capable of killing cells presenting their cognate peptide. These data indicate that HIV-1 infection leads to HERV expression and stimulation of a HERV-specific CD8+ T cell response. HERV-specific CD8+ T cells have characteristics consistent with an important role in the response to HIV-1 infection: a phenotype similar to that of T cells responding to an effectively controlled virus (cytomegalovirus), an inverse correlation with HIV-1 plasma viral load, and the ability to lyse cells presenting their target peptide. These characteristics suggest that elicitation of anti-HERV-specific immune responses is a novel approach to immunotherapeutic vaccination. As endogenous retroviral sequences are fixed in the human genome, they provide a stable target, and HERV-specific T cells could recognize a cell infected by any HIV-1 viral variant. HERV-specific immunity is an important new avenue for investigation in HIV-1 pathogenesis and vaccine design

Стилистический эффект разговорной речи и его составляющие

В обучении русскому языку как иностранному на современном этапе большое внимание уделяется особенностям русской разговорной речи. Это обусловлено целым рядом причин, среди которых, на наш взгляд, можно выделить следующие: во-первых, разговорная речь всегда отличается активностью проникновения во все сферы жизнедеятельности людей и функционирует как в повседневном общении, так и в различных сферах (литературе, кино, политике и т.д.). Во-вторых, разговорная речь носит многожанровый характер, что зачастую затрудняет ее понимание иностранными студентами. В-третьих, в разговорную речь помимо слов нейтрального стиля все активнее стала проникать арготическая лексика. Именно в связи с этим особый интерес у нас вызывает разговорный стиль речи в преломлении на инофонную аудиторию

Inhibition of Monkeypox virus replication by RNA interference

The Orthopoxvirus genus of Poxviridae family is comprised of several human pathogens, including cowpox (CPXV), Vaccinia (VACV), monkeypox (MPV) and Variola (VARV) viruses. Species of this virus genus cause human diseases with various severities and outcome ranging from mild conditions to death in fulminating cases. Currently, vaccination is the only protective measure against infection with these viruses and no licensed antiviral drug therapy is available. In this study, we investigated the potential of RNA interference pathway (RNAi) as a therapeutic approach for orthopox virus infections using MPV as a model. Based on genome-wide expression studies and bioinformatic analysis, we selected 12 viral genes and targeted them by small interference RNA (siRNA). Forty-eight siRNA constructs were developed and evaluated in vitro for their ability to inhibit viral replication. Two genes, each targeted with four different siRNA constructs in one pool, were limiting to viral replication. Seven siRNA constructs from these two pools, targeting either an essential gene for viral replication (A6R) or an important gene in viral entry (E8L), inhibited viral replication in cell culture by 65-95% with no apparent cytotoxicity. Further analysis with wild-type and recombinant MPV expressing green fluorescence protein demonstrated that one of these constructs, siA6-a, was the most potent and inhibited viral replication for up to 7 days at a concentration of 10 nM. These results emphasis the essential role of A6R gene in viral replication, and demonstrate the potential of RNAi as a therapeutic approach for developing oligonucleotide-based drug therapy for MPV and other orthopox viruses

Pennsylvania Folklife Vol. 18, No. 1

• Tanning in Chester County, Pennsylvania, 1711-1850 • Chester County Widow Wills (1714-1800), A Folklife Source • Folk Elements in Scotch-Irish Presbyterian Communities • The Thomas Massey House • Passengers on the Ketch Endeavour • The Medical Plants of Berks County, Pennsylvania • Notes and Documents: A Dunkard Love Feast ; Jacob Graeff\u27s Reminiscences of Reading • Symposium on the Pennsylvania Dutch Dialect • Farm Dress: Folk-Cultural Questionnaire No. 9https://digitalcommons.ursinus.edu/pafolklifemag/1033/thumbnail.jp