ANALISIS KELAYAKAN USAHA PENGOLAHAN ABON IKAN SKALA RUMAH TANGGA DI KOTA KUPANG

This study aims to determine the feasibility of abon fish processing businesshousehold scale in Kupang city.The method used is Quantitative Descriptive Method whilethe data in this study obtained through Questionnaire, Interview and Documentation.The results showed that the abon fish business conducted by community groups inKupang City has not been run efficiently yet, so it has not been able to provide benefits forthe group of fish abon businessmen in Kupang City today. Profit obtained is still very low orlow so it gives effect on the risk of loss in a certain period.From the result of this research, it is expected that abon fish processing group ofhousehold scale in Kupang city must increase the value of abon fish production from currentproduction or increase production per month must be bigger again with price of product /pack which equal to or bigger than BEP

Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses.

Extracellular RNAs (exRNAs) can be released by numerous cell types in vitro, are often protected within vesicles, and can modify recipient cell function. To determine how the composition and cellular sources of exRNAs and the extracellular vesicles (EVs) that carry them change in vivo during tissue inflammation, we analyzed bronchoalveolar lavage fluid (BALF) from mice before and after lung allergen challenge. In the lung, extracellular microRNAs (ex-miRNAs) had a composition that was highly correlated with airway-lining epithelium. Using cell type-specific membrane tagging and single vesicle flow, we also found that 80% of detected vesicles were of epithelial origin. After the induction of allergic airway inflammation, miRNAs selectively expressed by immune cells, including miR-223 and miR-142a, increased and hematopoietic-cell-derived EVs also increased >2-fold. These data demonstrate that infiltrating immune cells release ex-miRNAs and EVs in inflamed tissues to alter the local extracellular environment

Kawasan Wisata Kuliner Kuala Jengki Di Manado (Penerapan Konsep Place)

Sektor pariwisata merupakan salah satu potensi yang menjadi daya tarik bagi para wisatawan mancanegara. Banyak potensi wisata yang dapat dikembangkan di Indonesia seperti wisata alam, wisata sejarah, wisata budaya, dan wisata kuliner. Wisata kuliner adalah salah satu sektor pariwisata yang tak kalah menarik dengan sektor lainnya. Setiap daerah di Indonesia memiliki kuliner khas masing-masing, oleh karena pengaruh dari setiap budaya yang ada di daerah masing-masing tersebut, salah satunya kota Manado. Pada saat ini kota Manado telah memiliki sejumlah tempat yang menjual makanan-makanan khas sayangnya tempat-tempat tersebut kurang presentatif dan kurang menarik. Berangkat dari potensi dan permasalahan di atas maka dibutuhkankan perancanaan dan peracangan Kawasan Wisata Kuliner Kuala Jengki di Manado dengan penerapan konsep Place yang mengekspresikan dan menonjolkan keunikan potensi landscape yang berada di pinggiran sungai sehingga tempat ini mampu menampung kebutuhan kuliner khas yang lengkap bagi wisatawan serta tempat yang disertai dengan fasilitas hiburan dan rekreasi bagi masyarakat

Interval gangrene complicating superficial femoral artery stent placement

Interval gangrene—necrosis of tissue proximal to a successful distal revascularization procedure—is an exceeding rare complication. To date, only nine cases have been reported in the literature, and all were secondary to traditional open bypass procedures. We report the first case, to our knowledge, of interval gangrene after endovascular stent placement in the superficial femoral artery. We believe that with the increasing utilization of endovascular techniques to treat limb ischemia, the serious complication of interval gangrene must be revisited. Assessment of collateral circulation, precise stent placement, and the appropriate choice of stents and stent grafts will become increasing important as more and more of these lesions are treated with endovascular techniques

CD4 T lymphocyte autophagy is upregulated in the salivary glands of primary Sjögren’s syndrome patients and correlates with focus score and disease activity

Background: Primary Sjögren’s syndrome (pSS) is a common chronic autoimmune disease characterized by lymphocytic infiltration of exocrine glands and peripheral lymphocyte perturbation. In the current study, we aimed to investigate the possible pathogenic implication of autophagy in T lymphocytes in patients with pSS. Methods: Thirty consecutive pSS patients were recruited together with 20 patients affected by sicca syndrome a nd/or chronic sialoadenitis and 30 healthy controls. Disease activity and damage were evaluated according to SS disease activity index, EULAR SS disease activity index, and SS disease damage index. T lymphocytes were analyzed for the expression of autophagy-specific markers by biochemical, molecular, and histological assays in peripheral blood and labial gland biopsies. Serum interleukin (IL)-23 and IL-21 levels were quantified by enzyme-linked immunosorbent assay. Results: Our study provides evidence for the first time that autophagy is upregulated in CD4+ T lymphocyte salivary glands from pSS patients. Furthermore, a statistically significant correlation was detected between lymphocyte autophagy levels, disease activity, and damage indexes. We also found a positive correlation between autophagy enhancement and the increased salivary gland expression of IL-21 and IL-23, providing a further link between innate and adaptive immune responses in pSS. Conclusions: These findings suggest that CD4+ T lymphocyte autophagy could play a key role in pSS pathogenesis. Additionally, our data highlight the potential exploitation of T cell autophagy as a biomarker of disease activity and provide new ground to verify the therapeutic implications of autophagy as an innovative drug target in pSS

Kajian Pelaksanaan Pelayanan Cakupan K4 Di Puskesmas Baumata Tahun 2018

 K4 pregnant women services are complete antenatal services with a time of examination, namely one visit in the first trimester, one visit in the second trimester, and two visits in the third trimester. The Baumata Community Health Center experienced a decline in K4 coverage based on the Kupang District Health Office profile data and annual reports where in 2016 K4 coverage reached 60.7%, in 2017 it reached 51.8% and in 2018 it only reached 44.2%. This research is a combination of qualitative and quantitative research (mix method) with descriptive research. The purpose of this study is to examine the implementation of K4 coverage services at the Baumata community health center in 2018 which includes description of inputs (human resources, facilities, sources of funds, policies and Standard Operating Procedures), process descriptions (planning and organizing) as well as output descriptions (K4 coverage ). Informants in this study amounted to 13 people and respondents numbered 66 people consisting of the Head of the Family Health and Nutrition Section of the Kupang District Health Office, the Head of the Baumata community health center, the Midwife who provided K4 services and the mother who had a pregnancy (K4) at the community health center Baumata in 2018. The results showed that in terms of input at the Baumata community health center as a whole it was good but there was no Ultrasonography facility at the community health center and the limited space where there was no special room for maternal and child health services/ family planning/immunization, in terms of the process is good related to planning, organizing and forms of services received by pregnant women are in accordance with 10T standards, it's just a long time in the process of providing services due to poor management time and lack of awareness of pregnant women to check pregnancy to the facility. The nearest health bag is due to the lack of knowledge of pregnant women about the importance of antenatal care, while in terms of output based on document review it can be seen that in the past three years there has been a decline in achievement of the K4 target at the Baumata community health center. It is recommended that the Baumata community health center procure supporting facilities in the form of Ultrasonography and additional rooms, make time management so that pregnant women do not wait too long for the service process, and conduct socialization to the public about the importance of antenatal care

Recent trends in publication of basic science and clinical research by United States investigators in anesthesia journals

<p>Abstract</p> <p>Background</p> <p>United States anesthesia research production declined sharply from 1980-2005. Whether this trend has continued despite recent calls to improve output is unknown. We conducted an observational internet analysis to quantify American basic science and clinical anesthesia research output in 14 anesthesia journals with impact factors greater than one at three-year intervals during the past decade.</p> <p>Results</p> <p>American investigators published 1,486 (21.7%) of the total of 6,845 research articles identified in anesthesia journals in 2001, 2004, 2007, and 2010. Approximately two-thirds of all US articles were published in <it>Anesthesiology </it>and <it>Anesthesia and Analgesia</it>. There was a significant correlation (r²= 0.316; P = 0.036) between the number of articles published by American authors in each anesthesia journal and the corresponding journal's impact factor in 2010. Significantly (P < 0.05; Pearson's Chi-square) fewer basic science articles were published in 2007 and 2010 compared with 2001. US clinical research output also declined in 2007 (201; 15.7%) compared with 2001 (266; 19.1%) and 2004, but an increase occurred in 2010 (279; 21.8%, P < 0.05 versus 2007).</p> <p>Conclusions</p> <p>The results indicate that US anesthesia research output continued to decrease from 2001 to 2007. An increase in clinical but not basic science research was observed in 2010 compared with 2007, suggesting that a modest recovery in clinical research production may have begun.</p

Widespread translational control of fibrosis in the human heart by RNA-binding proteins

BACKGROUND: Fibrosis is a common pathology in many cardiac disorders and is driven by the activation of resident fibroblasts. The global post-transcriptional mechanisms underlying fibroblast-to-myofibroblast conversion in the heart have not been explored. METHODS: Genome-wide changes of RNA transcription and translation during human cardiac fibroblast activation were monitored with RNA sequencing and ribosome profiling. We then used an RNA-binding protein-based analyses to identify translational regulators of fibrogenic genes. The integration with cardiac ribosome occupancy levels of 30 dilated cardiomyopathy patients demonstrates that these post-transcriptional mechanisms are also active in the diseased fibrotic human heart. RESULTS: We generated nucleotide-resolution translatome data during the TGFβ1-driven cellular transition of human cardiac fibroblasts to myofibroblasts. This identified dynamic changes of RNA transcription and translation at several time points during the fibrotic response, revealing transient and early-responder genes. Remarkably, about one-third of all changes in gene expression in activated fibroblasts are subject to translational regulation and dynamic variation in ribosome occupancy affects protein abundance independent of RNA levels. Targets of RNA-binding proteins were strongly enriched in post-transcriptionally regulated genes, suggesting genes such as MBNL2 can act as translational activators or repressors. Ribosome occupancy in the hearts of patients with dilated cardiomyopathy suggested the same post-transcriptional regulatory network was underlying cardiac fibrosis. Key network hubs include RNA-binding proteins such as PUM2 and QKI that work in concert to regulate the translation of target transcripts in human diseased hearts. Furthermore, silencing of both PUM2 and QKI inhibits the transition of fibroblasts toward pro-fibrotic myofibroblasts in response to TGFβ1. CONCLUSIONS: We reveal widespread translational effects of TGFβ1 and define novel post-transcriptional regulatory networks that control the fibroblast-to-myofibroblast transition. These networks are active in human heart disease and silencing of hub genes limits fibroblast activation. Our findings show the central importance of translational control in fibrosis and highlight novel pathogenic mechanisms in heart failure