503 research outputs found

    Results of hysteroscopic management of endometrial pathology through laser diode in naval medical center: number of cases

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    Background: Hysteroscopy consists of an excellent technique for the diagnosis of uterine pathology, the diode laser for treatment has been included, which is energy in the form of a beam of light, monochromatic and coherent, equipped with a great capacity to cut tissues and vaporize them. Objective of this study was to know the result of the hysteroscopic treatment with diode laser, in patients of reproductive age with uterine pathology in the naval medical center of Mexico for a period of one year.Methods: A descriptive study was carried out in 8 patients, with abnormal uterine bleeding and uterine pathology, to know the result of the hysteroscopic treatment with diode laser, inclusion criteria were age of 18 to 40 years, uterine pathology, without previous treatments, exclusion criteria were pregnancy, medical treatment, does not accept the procedure, in a period of September 1, 2017 and September 1, 2018 at the Naval Medical Center of Mexico, the 45-watt 980 nm and 1470 wavelength diode laser console nm with 3fr extra flexible conical tip glass fibers.Results: The average age 29.9±8.5 years, without comorbidities; 60% had an initial diagnosis of endometrial thickening and abnormal uterine bleeding and 40% had a diagnosis of infertility, the most frequent pathology is an endometrial polyp, during the procedure none of the patients required analgesia or post-surgical sedation. The average bleeding was 24.44 ml.Conclusions: The hysteroscopic removal of uterine pathology with diode laser was performed without anesthesia, in an outpatient, feasible and safe way, the surgical time is reduced, with a follow-up of 12 months without recurrence

    Re-expansion of balloon-expandable stents after growth

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    AbstractObjectives. The purpose of this study was to evaluate the feasibility of re-expansion of balloon expandable intravascular stents and to examine the gross and histologic effects of re-expansion on vascular integrity.Background. Intravascular stents have been used successfully as an adjunct to balloon dilation of congenital pulmonary artery branch stenosis and postoperative stenosis of the pulmonary arteries in children. However, use of rigid stents in children could result in development of relative stenosis at the site of stent implantation with subsequent growth of the child.Methods. Stainless steel “iliac” stents were placed in the thoracic aorta of 10 normal juvenile swine by a transcatheter technique. Angiography and re-expansion were performed at a mean of 11 weeks (n = 9) and again at 18 weeks (n = 5). After euthanasia, the aortic specimens were removed for gross and histologic examination.Results. Stents were successfully implanted in 10 swine. Re-expansion was successfully performed in each animal at 11 weeks and at 18 weeks. Aortic growth produced a relative constriction of the aorta of 20% ± 10% (mean ± SD) at the site of stent implantation at both 11 and 18 weeks. Re-expansion produced a significant increase in mean stent diameter from 10.1 ± 1 mm to 12.3 ± 1.2 mm at 11 weeks and from 11.2 ± 0.7 to 13.5 ± 1.1 mm at 18 weeks after implantation (p < 0.001). Balloon dilation produced a relative increase in stent diameter of 21% ± 7% at 11 weeks and 18% ± 4% at 18 weeks. Stent re-expansion was accompanied by plastic deformation of the neointima without neointimal dissection. Where neointima was thick, there was no evidence of neointimal abrasion, but where neointima was thin, areas of localized neointimal abrasion were observed with focal fibrin and platelet adherence to the stent struts. There was no evidence of medial or adventitial hemorrhage or dissection produced by re-expansion.Conclusions. Re-expansion of intravascular stents is feasible after growth in juvenile swine without significant injury to neointima, media or adventitia. The results of this study support careful and selective use of intravascular stents as an adjunct to balloon dilation of congenital stenoses in children

    Comparison of DNA Fragmentation and Color Thresholding for Objective Quantitation of Apoptotic Cells

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    Apoptosis is a process of cell death characterized by distinctive morphological changes and fragmentation of cellular DNA. Using video imaging and color thresholding techniques, we objectively quantitated the number of cultured CD4 + T-lymphoblastoid cells (HUT78 cells, RH9 subclone) displaying morphological signs of apoptosis before and after exposure to Îł-irradiation. The numbers of apoptotic cells measured by objective video imaging techniques were compared to numbers of apoptotic cells measured in the same samples by sensitive apoptotic assays that quantitate DNA fragmentation. DNA fragmentation assays gave consistently higher values compared with the video imaging assays that measured morphological changes associated with apoptosis. These results suggest that substantial DNA frag-mentation can precede or occur in the absence of the morphological changes which are associated with apoptosis in Îł-irradiated RH9 cells

    Identification of miR-660-5p targets involved in breast cancer progression

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    Background: Breast cancer (BC) is the most diagnosed cancer in women globally. MicroRNAs (miRNAs) participate in different processes of BC; their deregulation can make them act as oncogenes or tumor suppressors, participating in cancer progression. Using the TCGA (The Cancer Genome Atlas) database, we found miR-660-5p significantly overexpressed and associated with poor survival in patients with this pathology. It is reported that miR-660-5p induces proliferation, migration, and invasion in BC. However, the specific targets of this miRNA that induce each of these processes are unknown. In this project we propose to identify the targets of miR-660-5p involved in proliferation, migration, invasion, and angiogenesis in BC cells. Methods: The basal levels of miR-660-5p were determined by RT-qPCR. The effect of miR-660-5p was evaluated on proliferation, invasion, and migration processes in MDA-MB-231 and MCF-7 cells, and angiogenesis in HUVEC cells transfected with the miR-660-5p inhibitor. We identified targets of miR-660-5p using different databases, and we evaluated their expression by RT-qPCR in plate. Results: In this study, we found that miR-660-5p is significantly upregulated in BC cells MDA-MB-231 and MCF-7, compared to normal breast cells MCF-10A. In addition, we observed a significantly decrease in the processes of proliferation, migration, and invasion in BC cells, compared to untreated cells and negative control group. Similarly, we observed a significantly decrease in the angiogenesis process in HUVEC cells, compared to untreated cells and negative control group. Likewise, by analyzing the different databases and the literature, we found a total of 28 miR-660-5p targets involved in oncological processes. Conclusions: miR-660-5p is overexpressed in BC cells compared to healthy breast cells. Furthermore, miR-660-5p induces the processes of proliferation, migration and invasion in BC cells, and angiogenesis in HUVEC cells

    A Directory of Cartographic Inventors: Clever People who were Awarded a US Patent for a Map-related Device or Method

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    As its title and subtitle imply, this book is a collection of short biographies of people awarded United States patents for inventions intended to improve map use or map making. We say “intended” because, as with most patented innovations, their clever ideas seldom made it to store shelves, magazine ads, or mail order catalogs—a fate shared with most improvements proposed in cartography’s scientific-technical journals. This collection is a spinoff of a project focused on inventions rather than inventors. The project’s principal product was Monmonier\u27s book Patents and Cartographic Inventions: A New Perspective for Map History, published in 2017 by Palgrave Macmillan. As its chapter titles confirm, the emphasis was on genres of innovation like route-following devices and map folding, rather than on their inventors, whose diverse life stories could too readily distract from a narrative focused on technological trends, clever ideas, and wider impacts

    Synbiotic therapy decreases microbial translocation and inflammation and improves immunological status in HIV-infected patients: a double-blind randomized controlled pilot trial

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    BACKGROUND: HIV-infection results in damage and dysfunction of the gastrointestinal system. HIV enteropathy includes pronounced CD4+ T-cell loss, increased intestinal permeability, and microbial translocation that promotes systemic immune activation, which is implicated in disease progression. A synbiotic is the combination of probiotics and prebiotics that could improve gut barrier function. Our study goal was to determine whether the use of a synbiotic, probiotics or a prebiotic can recover immunological parameters in HIV-infected subjects through of a reduction of microbial translocation and pro-inflammatory cytokine production. METHODS: A randomized, double-blind controlled study was performed; twenty Antiretroviral treatment-naïve HIV-infected subjects were subgrouped and assigned to receive a synbiotic, probiotics, a prebiotic, or a placebo throughout 16 weeks. RESULTS: We had no reports of serious adverse-events. From baseline to week 16, the synbiotic group showed a reduction in bacterial DNA concentrations in plasma (p = 0.048). Moreover, the probiotic and synbiotic groups demonstrated a decrease in total bacterial load in feces (p = 0.05). The probiotic group exhibited a significant increment of beneficial bacteria load (such as Bifidobacterium; p = 0.05) and a decrease in harmful bacteria load (such as Clostridium; p = 0.063). In the synbiotic group, the CD4+ T-cells count increased (median: +102 cells/μL; p = 0.05) and the level of Interleukin 6 cytokine decreased significantly (p = 0.016). CONCLUSIONS: Our study showed a significant increase in CD4+ T lymphocyte levels in the synbiotic group, which could delay the initiation of antiretroviral therapy and decrease costs in countries with limited resources
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