Maggot Debridement Therapy in the Treatment of Footrot and Foot Scald in Sheep

In sheep with acute and chronic interdigital skin inflammation, maggot therapy was used for the debridement and speeding up the healing process. Sheep were housed at the university farm Zemplínska Teplica and before using maggot therapy, they were unsuccessfully treated by a footbath containing 10% copper sulphate and topical application of oxytetracycline solution in alcohol. Six non-pregnant Valachian sheep between two to four years of age, weighing 43 to 57 kg were treated with maggots of Lucilia sericata (Meigen) (Diptera: Calliphoridae). The larval population should be 8-12 per square centimetre of wound, but all affected tissues were trimmed away before their application. The effect of a single application for 3-6 days was evaluated. Debridement was rapid and selective. The treatment was well tolerated by animals. New layers of healthy tissue were formed over the wounds. It was observed that maggots of L. sericata are capable of cleaning the wounds after single application

Efficacy of manuka honey with conventional antifungals on <i>Malassezia pachydermatis</i>

Yeast infections such as otitis externa and seborrheic dermatitis in dogs and cats are frequently associated with Malassezia pachydermatis secondary infection. It is part of the normal cutaneous microflora of most warm-blooded vertebrates, however, under certain conditions, it can become a causative agent of infection that needs to be treated pharmacologically. Azole derivatives are the drugs of the first choice. An interesting trend in developing resistance is the use of natural substances, which include manuka honey with confirmed antimicrobial properties. The main intention of this research was to evaluate the mutual effect of manuka honey in combination with four conventional azole antifungals – clotrimazole, fluconazole, itraconazole, and miconazole – on 14 Malassezia pachydermatis isolates obtained from dogs and 1 reference strain. A slightly modified M27–A3 method (CLSI 2008) and the checkerboard test (Nikolić et al. 2017) were used for this purpose. Our results show an additive effect of all 4 antifungals with manuka honey concurrent use. Based on the determined values of fractional inhibitory concentration index (FICI – 0.74±0.03 when manuka honey combined with clotrimazole, 0.96±0.08 with fluconazole, 1.0±0 with miconazole and 1.16±0.26 with itraconazole), it was found in all cases that the effect of substances used is more pronounced in mutual combination than when used separately

Tetanus in Animals — Summary of Knowledge

Tetanus is a neurologic non-transmissible disease (often fatal) of humans and other animals with a worldwide occurrence. Clostridium tetani is the spore producing bacillus which causes the bacterial disease. In deep penetrating wounds the spores germinate and produce a toxin called tetanospasmin. The main characteristic sign of tetanus is a spastic paralysis. A diagnosis is usually based on the clinical signs because the detection in the wound and the cultivation of C. tetani is very difficult. Between animal species there is considerable variability in the susceptibility to the bacillus. The most sensitive animal species to the neurotoxin are horses. Sheep and cattle are less sensitive and tetanus in these animal species are less common. Tetanus in cats and dogs are rare and dogs are less sensitive than cats. Clinically two forms of tetanus have been recognized, i. e. localized and generalized. The available treatment is not specific because the toxin in neuronal cells cannot be accessed by antitoxin antibodies. The aim of the therapy is to: neutralise the unbound neurotoxin, inhibit C. tetani growth in the wound, and provide supportive care to mitigate the effects of the neurotoxin. The treatment is difficult with an unclear prognosis

Performance Anxiety in Slovak Students at Adolescent and Young Age

The paper presents outcomes of a study on the levels of performance anxiety in Slovak school and university students in adolescence and early adulthood from a gender perspective and depending on their educational stage. The research sample consisted of 589 respondents, 270 students of secondary schools and 319 students of the Economic University. The data was collected using a standardized questionnaire KSAT [13] and brainstorming method. The following hypotheses were tested: 1) there are statistically significant differences in the level of anxiety between males and females; 2) performance anxiety decreases with age. Our research didn’t confirm the direct impact of sex on stage fright; nor did it prove that performance anxiety decreases with age. A statistically significant difference in the level of stage fright between males and females was found only in the second-year university students (p-value = 0.0001).In our pedagogical practice, we will focus on the development and implementation of special strategies for overcoming stage fright that would fit every person

Mycobiota and Mycotoxic Contamination of Feed Cereals

The cereals are a suitable substrate for the growth of microscopic filamentous fungi. Micromycetes are capable of reducing the nutritional value of feedstuff and they can produce several mycotoxins. The most frequent genera of microscopic filamentous fungi are Fusarium, Penicillium, Alternaria and Aspergillus. The contamination by microscopic fungi and mycotoxins was determinated in 56 samples of feed cereals originating from the Slovak Republic. The most common genera of fungi detected in the feed cereals included: Alternaria (67.8 %), Fusarium (44.6 %), Penicillium (39.2 %), Mucor (30.3 %), Rhizopus (28.5 %), Cladosporium (21.4 %), Scopulariopsis (8.9 %) and Aspergillus (1.7 %). Deoxynivalenol was present in 24 samples (42.8 %) and zearalenone in 15 samples (26.7 %). The values of both mycotoxins did not reach the regulatory limits and thus they do not pose a risk to livestock nutrition

Diagnostics of Malassezia Species: A Review

Yeasts from the genus Malassezia belongs to normal commensal skin flora of warm-blooded vertebrates. These yeasts may act as opportunistic pathogens and cause skin diseases in humans and animals under certain conditions. The identification of Malassezia species is based on the phenotypic or genotypic diagnostics. The methods used for the phenotypic identification is determined by: the growth on Sabouraud agar, growth on selective media (Leeming-Notman agar, Dixon agar, Chrom Malassezia agar), the ability to utilise different concentrations of Tween, monitoring of the growth on CEL agar (soil enriched with castor oil) and TE agar (Tween-esculine agar), and the catalase test. The genotypic identification uses molecular methods like: the pulsed field gel electrophoresis (PFGE), random amplified polymorphic DNA (RAPD), amplified fragment lenght polymorphism (AFLP), denaturing gradient gel electrophoresis (DGGE), and the DNA sequence analysis

In Vivo Assessment of Zearalenone Toxicity

The microscopic filamentous fungi of the genus Fusarium are capable of producing secondary metabolites—mycotoxins. Fusarium fungi synthesize trichothecenes, zearalenone (ZEA) and fumonisins under appropriate environmental conditions. In this biological experiment, we studied the effects of zearalenone on a model organism called Artemia franciscana. During the three-day in vivo tests, we used five different concentrations of zearalenone (0.08 ppm, 0.4 ppm, 2 ppm, 10 ppm and 50 ppm). The results of this study showed that as the zearalenone concentration and the duration of the mycotoxin exposure increased, the lethality of artemia also increased. Our study showed that the toxicity of zearalenone to Artemia franciscana was relatively low

Occurrence of Malassezia Yeasts in Dermatologically Diseased Dogs

The Malassezia genus is represented by several lipophilic yeasts, normally present on the skin of many warm-blooded vertebrates, including humans. The aim of this study was to investigate the occurrence of Malassezia yeasts in dogs with skin lesions (dermatitis, interdigital dermatitis and inflammation of anal sacs) and otitis externa. The presence of Malassezia spp. was investigated in a group of 300 dogs exhibiting clinical manifestations. The isolates of Malassezia were identified by using phenotypic (biochemical-physiological and morphological characteristics) and genotypic methods (PCR, RFLP-AluI, BanI and MspA1I) which allowed their precise identification. Malassezia yeasts were isolated from 84 specimens obtained from 76 positive dogs. M. pachydermatis was the most frequently isolated species (79 isolates) in this study. M. furfur was identified in four dogs and M. nana in one dog. The prevalence of isolated Malassezia spp. was 25.3 % in dogs with skin lesions; from which 36.0 % were dogs suffering from otitis externa, 24.5 % from dogs having dermatitis, 16.4 % from dogs with interdigital dermatitis and 14.3 % from dogs having inflammation of the anal sacs. A higher prevalence of Malassezia spp. was observed in animals with pendulous ears in comparison with dogs having erect ears

Antifungal Susceptibility of Malassezia pachydermatis Isolates from Dogs

The genus Malassezia belongs to Basidiomycota and includes 16 species, from which M. pachydermatis is the most common in dogs. M. pachydermatis is a member of the normal mycobiota of the skin and mucosal sites of dogs. Under certain conditions, these yeasts can be opportunistic pathogens and involved skin and ear canal infections of these animals. Topical and oral antifungal agents are used for the therapy of Malassezia dermatitis and otitis. With the expanding use of antifungal agents, resistant strains of Malassezia are increasingly detected. In this study, the susceptibility of 40 M. pachydermatis isolates to fluconazole, itraconazole, ketoconazole, clotrimazole and nystatin were evaluated in vitro based on the modified standard disk diffusion method M44-2A

Antifungal Effect of Selected Essential Oils on Malassezia pachydermatis Growth

The antifungal activities of 14 selected essential oils (at the concentrations of 0.5 %, 5 %, and 30 %) against the yeast Malassezia pachydermatis (18 isolates and one reference strain) were investigated. The isolates of M. pachydermatis were obtained from swabs of external ear canals of healthy dogs using sterile swabs. The determination of the efficacy was based on a modified disc diffusion method (CLSI M44-A2). The best antifungal efficacy (100 %) was shown by clove, cinnamon and oregano at the concentration of 30 %; less significant efficacy was shown at the concentration of 5 % (38 %, 33 % and 5 %, respectively). Satureja inhibited the growth of Malassezia (efficacy of 16 %) only at the concentration of 30 %. Bergamot, lavender, juniper, cedar, sage, tea-tree, grapefruit, pine, chamomile and yarrow essential oils were not able to form inhibition zones as defined in the methodology used (greater or equal to 15 mm) in all concentrations used. Therefore, according to the interpretation criterion, they were considered ineffective. In all cases, the concentration of 0.5 % was not effective against the growth of Malassezia yeasts