Dataset Bastiaans et al. 2015 Evolution

Dataset Bastiaans et al. 2015 Evolutio

Most parsimonous tree based on combined data set of <i>ITS</i>, <i>β-tubulin</i> and <i>calmodulin</i> sequences.

<p>sequences are from 18 selected isolates and close taxa using MEGA 7 software, showing the relationship among our isolates and <i>Paecilomyces formosus</i> isolates, especialy to <i>P</i>. <i>lecythidis</i>. The percentages above and below the branches are the frequencies of a given branch appeared in 1000 bootstrap replications. Bootstrap values less than 50% are not displayed. <i>Thermoascus crustaceus</i> has been used as outgroup.</p

Source of used isolates in phylogeny.

<p>Source of used isolates in phylogeny.</p

Most parsimonous tree based on combined data set of <i>ITS</i>, <i>β-tubulin</i> and <i>calmodulin</i> sequences.

<p>sequences are from 62 selected isolates and close taxa using MEGA 7 software, showing low variation among our isolates. Isolates from <i>Pistacia vera</i>, Isolates from <i>Pistacia mutica</i>, Isolates from <i>Punica granatum</i>, Isolates from <i>Tamarix hispida</i>, Isolates from <i>Tamarix aphylla</i>, Isolates from <i>Prunus amygdalus</i>, Isolates from <i>Haloxylon</i> sp., Isolates from <i>Nerium oleander</i>, Isolates from <i>Caesalpinia gilliesii</i> and Isolates from the air of pistachio orchards. The percentages above and below the branches are the frequencies of a given branch appeared in 1000 bootstrap replications. Bootstrap values less than 50% are not displayed. <i>Paecilomyces formosus</i> CBS990.73B has been used as outgroup.</p

Obtained isolates of <i>Paecilomyces</i>.

<p>Obtained isolates of <i>Paecilomyces</i>.</p

Pathogenicity test.

<p>(A) Inoculation of <i>P</i>. <i>formosus</i> on two years old twigs of pistachio in orchard. Fungus development and canker formation are evident. The fungus develops along the axis of the branch. (B) Control inoculation and callus formation in pathogen free scar.</p

Acid production on CREA.

<p>(A) Creatine sucrose agar (CREA) plate inoculated with isolate Pf623 which was unable to grow and produce acid, hence an unchanged purple plate color and (B) CREA plate inoculated with isolate Pf613 which was able to grow and produce acid at 25°C for seven days in continuous dark condition; acid production indicated by the color change to yellow.</p

Strains and their sequences from GenBank (NCBI) used in phylogenetic analysis.

<p>Strains and their sequences from GenBank (NCBI) used in phylogenetic analysis.</p

data on mycelial growth, nodulation and genotyping

The file contains data on Termitomyces mycelial growth rate and asexual spore yield, and on genotyping data from the mixing experiment

dataset Bastiaans et al. (2014)

These are the data collected and used for the publication "Regular bottlenecks and restrictions to somatic fusion prevent the accumulation of mitochondrial defects in Neurospora". For further details please read the paper