Taqman primers/probes used for qPCR analysis of pan GR mRNA and GR exon 1 mRNA transcript variants. HK- housekeeping gene.

<p>Taqman primers/probes used for qPCR analysis of pan GR mRNA and GR exon 1 mRNA transcript variants. HK- housekeeping gene.</p

Endpoint PCR primers used for detection of GR exon 1 mRNA transcript variants.

<p>Endpoint PCR primers used for detection of GR exon 1 mRNA transcript variants.</p

Effect of suicide on expression of GR mRNA transcripts in the DLPFC.

<p>Suicide-positive and suicide-negative control cases, bipolar disorder cases and schizophrenia cases were compared. A) Pan GR mRNA expression; B) GR-1B mRNA expression; C) GR-1C mRNA expression; D) GR-1H mRNA expression. For all transcripts, GR transcript expression was decreased in suicide-negative schizophrenia cases relative to suicide-positive schizophrenia cases and relative to controls (all suicide-negative). For the GR-1C mRNA transcript, expression was decreased in bipolar disorder as well as schizophrenia in suicide-negative cases relative to both suicide-positive cases and controls. Green, orange and blue diamonds represent control, bipolar disorder and schizophrenia cases respectively. Red bars represent group means. Abbreviations: BP- bipolar disorder, SCZ- schizophrenia. * <i>p</i>≤0.05, ** <i>p</i><0.005, *** <i>p</i><0.0005.</p

Effect of rs41423247 (Bcl1) genotype on GRα protein expression in the DLPFC.

<p>There were significant main effects of genotype on IR band 2 intensity (ANCOVA, F(2, 87) = 4.85, <i>p</i><0.05) and total IR bands 1–5 (ANOVA F(2, 86) = 3.28, <i>p</i><0.05). The abundance of IR band 2 (67 kDa GRα) was significantly decreased in rs41423247 GG homozygotes (n = 11; 26.5% decrease, <i>p</i><0.05) and GC heterozygotes (n = 43; 23.4% decrease, <i>p</i><0.01) relative to CC homozygotes (n = 41). The total GRα abundance (IR bands 1–5) was decreased (15.1%) in GC heterozygotes relative to CC homozygotes (<i>p</i><0.05). * p<0.05, ** p = 0.006.</p

Demographic details of cases from the Stanley Array cohort used to quantify GR exon 1 mRNA transcript variant expression.

<p>Abbreviations: M- male, F- female, L- left, R- right, PMI- post-mortem interval, RIN- RNA integrity number. Data quoted are mean (range) +/− standard deviation.</p

Effects of rs10052957 (Tth111l) and rs6190 (R23K) genotypes on GR mRNA expression.

<p>A) A significant main effect of rs10052957 genotype on GR-1B mRNA expression was identified (ANCOVA F(2, 81) = 5.32, <i>p</i><0.001), with significant decreases in GR-1B expression in individuals with CC genotype (n = 46) relative to those with TC genotype (n = 40; 18.4% decrease, <i>p</i><0.05) and TT genotype (n = 9; 31.8% decrease, <i>p</i><0.005). B) An effect of rs6190 genotype on GR-1C mRNA expression was also observed (Mann-Whitney U-test, z = 2.58, <i>p</i><0.01), with a 24.8% decrease in GG homozygotes (n = 91) relative to GA heterozygotes (n = 4). Error bars represent SEM. * <i>p</i><0.05, ** <i>p</i><0.005.</p

Details of NR3C1 SNPs genotyped in this study.

<p>Abbreviations: UCSC- University of California Santa Cruz, MAF- minor allele frequency, HWE- Hardy-Weinberg Equilibrium.</p

Endpoint PCR detection of GR exon 1 mRNA transcript variants in the DLPFC.

<p>A) strong amplification of GR-1B, GR-1C and GR-1H variants was evident in DLPFC cDNA, B) weaker amplification of GR-1E and GR-1F variants was observed in the DLPFC, but strong amplification was observed in universal human cDNA, C) GR-1A and GR-1D variants were detected neither in the DLPFC nor in universal cDNA. UNI- universal human cDNA.</p

Additional file 1: of Effects of sex and DTNBP1 (dysbindin) null gene mutation on the developmental GluN2B-GluN2A switch in the mouse cortex and hippocampus

Supporting information. Figure S1. Synaptophysin abundance in membrane and PSD fractions from the frontal cortex across postnatal development. Figure S2. Representative images of blots used for quantification of NMDA receptor signaling proteins in the hippocampus. Figure S3. Loading standard curves for PSD fractions from the frontal cortex. Figure S4. Loading standard curves for membrane fractions from the frontal cortex. Figure S5. Loading standard curves for PSD fractions from the hippocampus, using P7 samples only. Figure S6. Loading standard curves for PSD fractions from the hippocampus, using P56 samples only. Figure S7. A–G Main effects of age, not described in main text, for proteins quantified in this study; H, I For SRC and PLCγ, distinct developmental trajectories were seen in different subcellular compartments, with stable SRC and PLCγ levels seen in PSD enrichments despite significantly decreasing levels in membranes in general. PSD- postsynaptic density, membranes- crude synaptic membranes (cSM), FCx- frontal cortex, HIP- hippocampus. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.00005, *****p < 1 × 10−5. (DOCX 1627 kb