Efficacy of metabarcoding for identification of fish eggs evaluated with mock communities.

There is urgent need for effective and efficient monitoring of marine fish populations. Monitoring eggs and larval fish may be more informative than that traditional fish surveys since ichthyoplankton surveys reveal the reproductive activities of fish populations, which directly impact their population trajectories. Ichthyoplankton surveys have turned to molecular methods (DNA barcoding & metabarcoding) for identification of eggs and larval fish due to challenges of morphological identification. In this study, we examine the effectiveness of using metabarcoding methods on mock communities of known fish egg DNA. We constructed six mock communities with known ratios of species. In addition, we analyzed two samples from a large field collection of fish eggs and compared metabarcoding results with traditional DNA barcoding results. We examine the ability of our metabarcoding methods to detect species and relative proportion of species identified in each mock community. We found that our metabarcoding methods were able to detect species at very low input proportions; however, levels of successful detection depended on the markers used in amplification, suggesting that the use of multiple markers is desirable. Variability in our quantitative results may result from amplification bias as well as interspecific variation in mitochondrial DNA copy number. Our results demonstrate that there remain significant challenges to using metabarcoding for estimating proportional species composition; however, the results provide important insights into understanding how to interpret metabarcoding data. This study will aid in the continuing development of efficient molecular methods of biological monitoring for fisheries management

Gauging Support for Innovative Farmland Preservation Techniques

This paper describes four innovative farmland preservation techniques and gauges support through interviews of key stakeholders: program administrators, lawmakers, and landowners. Four techniques were selected for assessment from approximately 30 novel techniques: rights of first refusal; term conservation easements; land preservation tontines; and agricultural conservation pension. Rights of first refusal was the most favored, although respondents thought effective implementation would need targeting of land and a dedicated funding source. Agricultural conservation pension was also viewed favorably, although considered administratively difficult to implement. Tontines were perceived to be an interesting concept, but confusing, difficult to implement, and ill-defined. Term easements were viewed unfavorably because they did not preserve land permanently.Land Economics/Use,

Asymptotic Improvements of Lower Bounds for the Least Common Multiples of Arithmetic Progressions

For relatively prime positive integers $u_0$ and $r$, we consider the least common multiple $L_n:=\mathrm{lcm}(u_0,u_1,\ldots, u_n)$ of the finite arithmetic progression $\{u_k:=u_0+kr\}_{k=0}^n$. We derive new lower bounds on $L_n$ which improve upon those obtained previously when either $u_0$ or $n$ is large. When $r$ is prime, our best bound is sharp up to a factor of $n+1$ for $u_0$ properly chosen, and is also nearly sharp as $n\to\infty$.Comment: 10 page

An Age-Period-Cohort Database of Inter-Regional Migration in Australia and Britain, 1976-96

Report prepared as part of a collaborative project on "Migration Trends in Australia and Britain: Levels and Trends in an Age-Period-Cohort Framework" funded by the Economic and Social Research Council and the Australian Research Council. This paper describes the way in which parallel databases of inter-regional migration flows for Australia and Britain, classified by five year ages and birth cohorts for four five year periods between 1976 and 1996. The data processing involves estimation of migration data for comparable spatial units, the reduction of the number of those units to a reduced set for ease of analysis, the extraction of migration data from official data files supplied by the Australian Bureau of Statistics and the Office for National Statistics, and the filling of gaps in these files through iterative proportional fitting for some of the British data. The final stage in preparation of the migration databases was to estimate the numbers of transitions (Australia) or movements (Britain) for age-period-cohort spaces. In principle, this last estimation involves a fairly simple interpolation or aggregation of age-time classified migration data, but in practice a great deal of detailed attention is required. A final section specifies the populations at risk to be used for each age-period-cohort observation plan to compute migration intensities

Harmonising Databases for the Cross National Study of Internal Migration: Lessons from Australia and Britain

This project involves the development of a Web interface to origin-destination statistics from the 1991 Census (in a form that will be compatible with planned 2001 outputs). It provides the user with a set of screen-based tools for setting the parameters governing each data extraction (data set, areas, variables) in the form of a query. Traffic light icons are used to signal what the user has set so far and what remains to be done. There are options to extract different types of flow data and to generate output in different formats. The system can now be used to access the interaction flow data contained in the 1991 Special Migration Statistics Sets 1 and 2 and Special Workplace Statistics Set C. WICID has been demonstrated at the Origin-Destination Statistics Roadshows organised by GRO Scotland and held during May/June 2000 and the Census Offices have expressed interest in using the software in the Census Access Project

Use of a Producer Survey to Reconcile Differences in Experiment Station Yield Estimates

Average producer practice reveals that the expected returns are greater from dual-purpose wheat grown for both forage and grain than for grain-only wheat. Variety trials report an 11 bushel per acre yield advantage and hence economic advantage for grain-only. Research was conducted to reconcile the inconsistency.direct elicitation method, dual-purpose, simulation, stocker steers, survey, triangular distribution, wheat, Farm Management, Livestock Production/Industries, Q10, Q12,

Summer Workshop on Near-Earth Resources

The possible large scale use of extraterrestrial resources was addressed, either to construct structures in space or to return to Earth as supplements for terrestrial resources. To that end, various specific recommendations were made by the participants in the summer study on near-Earth resources, held at La Jolla, California, 6 to 13 August, 1977. The Moon and Earth-approaching asteroids were considered. Summaries are included of what is known about their compositions and what needs to be learned, along with recommendations for missions designed to provide the needed data. Tentative schedules for these projects are also offered

THE CAUSAL STRUCTURE OF LAND PRICE DETERMINANTS

This paper investigates causation contemporaneously and over time to elucidate the persistent lack of agreement about what "causes" changes in farmland prices. Using recently developed causal modeling framework of directed acyclic graphs (DAGs) and cointegrated (VAR) techniques, the assumed causal structures of existing structural and empirical models are tested directly. The results validate concerns about the nonstationarity of these series. Land price changes are found to respond to a small subset of the oft-cited causes of price change, including macroeconomic variables.Land Economics/Use,

A Conjoint Analysis of Public Preferences for Agricultural Land Preservation

Public preferences for the nonmarket services of permanently preserved agricultural land are measured and compared using conjoint analysis. The results from a survey of 199 Delawareans suggest environmental and nonmarket-agricultural services are the most important preserved-land attributes. Results also suggest that open space associated with wetlands on farms is neither an amenity nor a disamenity. On the margin, preserved parcels with agricultural and environmental attributes provide net benefits, which may exceed $1,000,000 for a 1,000-acre parcel. Preserved forestland provides benefits per acre that are statistically equivalent to cropland, though forestland may be less expensive to preserve.Land Economics/Use,

Editors' Note

Marketing,