Gene Expression during Survival of Escherichia coli O157:H7 in Soil and Water

The in vitro survival of Escherichia coli O157:H7 at 15°C under two experimental conditions (sterile soil and sterile natural water) was examined. DNA microarrays of the entire set of E. coli O157:H7 genes were used to measure the genomic expression patterns after 14 days. Although the populations declined, some E. coli O157:H7 cells survived in sterile stream water up to 234 days and in sterile soil for up to 179 days. Cells incubated in soil microcosms for 14 days expressed genes for antibiotic resistance, biosynthesis, DNA replication and modification, metabolism, phages, transposons, plasmids, pathogenesis and virulence, antibiotic resistance, ribosomal proteins, the stress response, transcription, translation, and transport and binding proteins at significantly higher levels than cells grown in Luria broth. These results suggest that E. coli O157:H7 may develop a different phenotype during transport through the environment. Furthermore, this pathogen may become more resistant to antibiotics making subsequent infections more difficult to treat

Analysis of language development and reading comprehension of learning disabled and chapter 1 students

The purpose of this study was to investigate the relationships between language development and reading ccmprehension found among thirty-three learning disabled students (LD) and thirty-three students in programs supported by Chapter 1 funds. These sixty-six subjects were in grades three through five in eleven elementary schools in Anderson Community Schools, Anderson, Indiana.The relationships between the language features of syntax and semantics, the language systems of listening and speaking and the levels of reading comprehension were assessed with the Test of Language Development - Intermediate (TOLD-I) and the Gates-MacGinitie Reading Tests, Level B Form 1. The .05 level of significance was set for acceptance of sixteen null hypotheses.The multivariate analysis of variance, used to determine if an overall difference existed between the groups on the assessment measure, indicated that such a difference did not exist. The two-way analysis of variance between the dependent variables of reading comprehension extended scaled scores and the TOLD-I quotient scores for the LD and Chapter 1 students did not yield significant differences. The Pearson product-moment correlation yielded statistical significance but low correlation coefficients (below .40) for the Gates-MacGinitie Reading Test (comprehension subtest) extended scaled scores and the TOLD-I quotients of Speaking, Semantics, Syntax, and Spoken language. All sixteen null hypothese were not rejected.Thesis (D. Ed.

Analysis of language development and reading comprehension of learning disabled and chapter 1 students

The purpose of this study was to investigate the relationships between language development and reading ccmprehension found among thirty-three learning disabled students (LD) and thirty-three students in programs supported by Chapter 1 funds. These sixty-six subjects were in grades three through five in eleven elementary schools in Anderson Community Schools, Anderson, Indiana.The relationships between the language features of syntax and semantics, the language systems of listening and speaking and the levels of reading comprehension were assessed with the Test of Language Development - Intermediate (TOLD-I) and the Gates-MacGinitie Reading Tests, Level B Form 1. The .05 level of significance was set for acceptance of sixteen null hypotheses.The multivariate analysis of variance, used to determine if an overall difference existed between the groups on the assessment measure, indicated that such a difference did not exist. The two-way analysis of variance between the dependent variables of reading comprehension extended scaled scores and the TOLD-I quotient scores for the LD and Chapter 1 students did not yield significant differences. The Pearson product-moment correlation yielded statistical significance but low correlation coefficients (below .40) for the Gates-MacGinitie Reading Test (comprehension subtest) extended scaled scores and the TOLD-I quotients of Speaking, Semantics, Syntax, and Spoken language. All sixteen null hypothese were not rejected.Thesis (D. Ed.

Suppression of the loss of an electroactive dopant from polypyrrole by using a non-aqueous electrolyte of dopant-phobicity

Loss of electroactive dopants from conducting polymers (CPs) was investigated by electrochemical quartz crystal microgravimetry (EQCM). pPy[ABTS], polypyrrole doped with ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate), was used as a model system. Two different kinds of electrolytes were used for studying the loss of dopants under electrochemical stimuli: one is a solvent to dissolve the dopant (dopant-philic) while the dopant is insoluble in the other (dopant-phobic). Degradation of polypyrrole backbone as well as loss of the dopant from pPy[ABTS] was observed in the dopant-philic electrolyte. Severe chemical overoxidation of polypyrrole by the most oxidized state of ABTS {(ABTS(2+))(2-)} was emphasized as a factor responsible for de-doping in addition to potential-driven overoxidation or ion exchange. In the dopant-phobic electrolyte, however, the chemical degradation of the polymer film was suppressed.close