INTEGRATED MICROFLUIDIC DEVICE FOR DROPLET MANIPULATION

Droplets based microfluidic systems have a big potential for the miniaturization of processes for bioanalysis. In the form of droplets, reagents are used in discrete volume, enabling high-throughput chemical reactions as well as single-cell encapsulation. Microreactors of this type can be manipulated and applied in bio-testing. In this work we present a platform for droplet generation and manipulation by using dielectrophoresis force. This platform is an integrated microfluidic device with a dielectrophoresis (DEP) chip. The microfluidic device generates microdroplets such as water in oil emulsion

Lens aberration compensation in interference microscopy

Emergence of products that feature functional surfaces with complex geometries, such as freeform optics in consumer electronics and augmented reality and virtual reality, requires high-accuracy non-contact surface measurement. However, large discrepancies are often observed between the measurement results of optical methods and contact stylus methods, especially for complex surfaces. For interference microscopy, such as coherence scanning interferometry, the three-dimensional surface transfer function provides information about the instrument spatial frequency passband and about lens aberrations that can result in measurement errors. Characterisation and phase inversion of the instrument’s three-dimensional surface transfer function yields an inverse filter that can be applied directly to the three-dimensional fringe data. The inverse filtering is shown to reduce measurement errors without using any data processing or requiring any a priori knowledge of the surface. We present an experimental verification of the characterisation and correction process for measurements of several freeform surfaces and an additive manufactured surface. Corrected coherence scanning interferometry measurements agree with traceable contact stylus measurements to the order of 10 nm

Foundation Design of the Athletics Stadium in Pilsner

Úkolem bakalářské práce je popsat návrh bezpečného a ekonomického založení tribuny atletického stadionu v Plzni. Návrh a posouzení je vypracován na základě zjištěných geotechnických podmínek a zatížení od konstrukce. Při výpočtech je postupováno dle platných norem a návrhových přístupů. Dále se práce věnuje technologii a postupu prací vrtaných velkoprůměrových pilot.The task of thisbachelor dissertation is to describe the concept of safe and economic foundation of the athletic stadium in Plzeň, the Czech Republic. The concept and assessment are developer on the basis of obtained geotechnical conditions and the load of the construction. The calculations are based upon valid forms and the concept approaches. Furthermore, the thesis focuses on technology and working proces of large diameter bored piles.

Laser-assisted morphing of complex three dimensional objects

Morphing refers to the smooth transition from a specific shape into another one, in which the initial and final shapes can be significantly different. A typical illustration is to turn a cube into a sphere by continuous change of shape curvatures. Here, we demonstrate a process of laser-induced morphing, driven by surface tension and thermally-controlled viscosity. As a proof-of-concept, we turn 3D glass structures fabricated by a femtosecond laser into other shapes by locally heating up the structure with a feedback-controlled CO2 laser. We further show that this laser morphing process can be accurately modelled and predicte

Visualization 2: Laser-assisted morphing of complex three dimensional objects

Media 2 Originally published in Optics Express on 29 June 2015 (oe-23-13-17355

Visualization 1: Laser-assisted morphing of complex three dimensional objects

Media 1 Originally published in Optics Express on 29 June 2015 (oe-23-13-17355

Ultrafast thin-disk laser oscillators as driving sources for high harmonic generation

Thin-disk laser oscillators can nowadays reach few tens of femtosecond pulses at gigawatt-level intracavity powers and megahertz-repetition rates becoming increasingly more powerful sources for intra-oscillator high harmonic generation (HHG). Currently, we can generate high harmonics in neon reaching photon energies of 70 eV, which we expect to increase toward 100 eV in the near future. In parallel, the achievable average and peak output powers of these oscillators in the range of 100 W and 100 MW, respectively, make these sources very promising to drive HHG in single-pass configuration after nonlinear pulse compression. Starting from transform-limited 30 to 50-fs soliton output soliton pulses of TDL oscillators, we will likely see these lasers approaching a single-cycle regime becoming highly attractive sources for attosecond science