Cool bottom processes on the thermally-pulsing AGB and the isotopic composition of circumstellar dust grains

(Abridged) We examine the effects of cool bottom processing (CBP) on several isotopic ratios in the convective envelope during the TP-AGB phase of evolution in a 1.5 M_sun initial-mass star of solar initial composition. We use a parametric model which treats extra mixing by introducing mass flow between the convective envelope and the underlying radiative zone. The parameters of this model are the mass circulation rate (Mdot) and the maximum temperature (T_P) experienced by the circulating material. The effects of nuclear reactions in the flowing matter were calculated using a set of structures of the radiative zone selected from a complete stellar evolution calculation. The compositions of the flowing material were obtained and the resulting changes in the envelope determined. Abundant ^26Al was produced by CBP for log T_P > 7.65. While ^26Al/^27Al depends on T_P, the isotopic ratios in CNO elements depend dominantly on the circulation rate. The correspondence is shown between models of CBP as parameterized by a diffusion formalism within the stellar evolution model and those using the mass-flow formalism employed here. The isotopic ratios are compared with the data on circumstellar dust grains. It is found that the ratios ^{18}O/^{16}O, ^{17}O/^{16}O, and ^26Al/^27Al observed for oxide grains formed at C/O < 1 are reasonably well-understood. However, the ^15N/^14N, ^12C/^13C, and ^26Al/^27Al in carbide grains (C/O > 1) require many stellar sources with ^14N/^15N at least a factor of 4 below solar. The rare grains with ^12C/^13C < 10 cannot be produced by any red-giant or AGB source.Comment: 35 pages, plus 18 included figures. Scheduled for January 10, 2003 issue of Ap

An analysis of ultraviolet spectra of Extreme Helium Stars and new clues to their origins

Abundances of about 18 elements including the heavy elements Y and Zr are determined from Hubble Space Telescope Space Telescope Imaging Spectrograph ultraviolet spectra of seven extreme helium stars (EHes): LSE 78, BD+10 2179, V1920 Cyg, HD 124448, PV Tel, LS IV -1 2, and FQ Aqr. New optical spectra of the three stars -- BD+10 2179, V1920 Cyg, and HD 124448 were analysed. The abundance analyses is done using LTE line formation and LTE model atmospheres especially constructed for these EHe stars. The stellar parameters derived from an EHe's UV spectrum are in satisfactory agreement with those derived from its optical spectrum. Adopted abundances for the seven EHes are from a combination of the UV and optical analyses. Published results for an additional ten EHes provide abundances obtained in a nearly uniform manner for a total of 17 EHes, the largest sample on record. The initial metallicity of an EHe is indicated by the abundance of elements from Al to Ni; Fe is adopted to be the representative of initial metallicity. Iron abundances range from approximately solar to about one-hundredth of solar. Clues to EHe evolution are contained within the H, He, C, N, O, Y, and Zr abundances. Two novel results are (i) the O abundance for some stars is close to the predicted initial abundance yet the N abundance indicates almost complete conversion of initial C, N, and O to N by the CNO-cycles; (ii) three of the seven stars with UV spectra show a strong enhancement of Y and Zr attributable to an s-process. The observed compositions are discussed in light of expectations from accretion of a He white dwarf by a CO white dwarf.Comment: 126 pages, 15 figures, 20 Tables, accepted for publication in the Ap

Reduced innate immune response to a Staphylococcus aureus small colony variant compared to its wild-type parent strain

Background: Staphylococcus aureus (S. aureus) small colony variants (SCVs) can survive within the host intracellular milieu and are associated with chronic relapsing infections. However, it is unknown whether host invasion rates and immune responses differ between SCVs and their wild-type counterparts. This study used a stable S. aureus SCV (WCH-SK2SCV) developed from a clinical isolate (WCH-SK2WT) in inflammation-relevant conditions. Intracellular infection rates as well as host immune responses to WCH-SK2WT and WCH-SK2SCV infections were investigated. Method: NuLi-1 cells were infected with either WCH-SK2WT or WCH-SK2SCV, and the intracellular infection rate was determined over time. mRNA expression of cells infected with each strain intra- and extra-cellularly was analyzed using a microfluidic qPCR array to generate an expression profile of thirty-nine genes involved in the host immune response. Results: No difference was found in the intracellular infection rate between WCH-SK2WT and WCH-SK2SCV. Whereas, extracellular infection induced a robust pro-inflammatory response, intracellular infection elicited a modest response. Intracellular WCH-SK2WT infection induced mRNA expression of TLR2, pro-inflammatory cytokines (IL1B, IL6, and IL12) and tissue remodeling factors (MMP9). In contrast, intracellular WCH-SK2SCV infection induced up regulation of only TLR2. Conclusions: Whereas, host intracellular infection rates of WCH-SK2SCV and WCH-SK2WT were similar, WCH-SK2SCV intracellular infection induced a less widespread up regulation of pro-inflammatory and tissue remodeling factors in comparison to intracellular WCH-SK2WT infection. These findings support the current view that SCVs are able to evade host immune detection to allow their own survival.Judy J.J. Ou, Amanda J. Drilling, Clare Cooksley, Ahmed Bassiouni, Stephen P. Kidd, Alkis J. Psaltis, Peter J. Wormald and Sarah Vreugd

Activity of bacteriophages in removing biofilms of pseudomonas aeruginosa isolates from chronic rhinosinusitis patients

Introduction: Pseudomonas aeruginosa infections are prevalent amongst chronic rhinosinusitis (CRS) sufferers. Many P. aeruginosa strains form biofilms, leading to treatment failure. Lytic bacteriophages (phages) are viruses that infect, replicate within, and lyse bacteria, causing bacterial death. Aim: To assess the activity of a phage cocktail in eradicating biofilms of ex vivo P.aeruginosa isolates from CRS patients. Methods: P. aeruginosa isolates from CRS patients with and without cystic fibrosis (CF) across three continents were multi-locus sequence typed and tested for antibiotic resistance. Biofilms grown in vitro were treated with a cocktail of four phages (CT-PA). Biofilm biomass was measured after 24 and 48 h, using a crystal violet assay. Phage titrations were performed to confirm replication of the phages. A linear mixed effects model was applied to assess the effects of treatment, time, CF status, and multidrug resistance on the biomass of the biofilm. Results: The isolates included 44 strain types. CT-PA treatment significantly reduced biofilm biomass at both 24 and 48 h post-treatment (p < 0.0001), regardless of CF status or antibiotic resistance. Biomass was decreased by a median of 76% at 48 h. Decrease in biofilm was accompanied by a rise in phage titres for all except one strain. Conclusion: A single dose of phages is able to significantly reduce biofilms formed in vitro by a range of P.aeruginosa isolates from CRS patients. This represents an exciting potential and novel targeted treatment for P. aeruginosa biofilm infections and multidrug resistant bacteria.Stephanie A. Fong, Amanda Drilling, Sandra Morales, Marjolein E. Cornet, Bradford A. Woodworth, Wytske J. Fokkens, Alkis J. Psaltis, Sarah Vreugde and Peter-John Wormal

Long-term X-ray variability of the microquasar system LS 5039/RX J1826.2-1450

We report on the results of the spectral and timing analysis of a BeppoSAX observation of the microquasar system LS 5039/RX J1826.2-1450. The source was found in a low-flux state with Fx(1-10 keV)= 4.7 x 10^{-12} erg cm^{-2} s^{-1}, which represents almost one order of magnitude lower than a previous RXTE observation 2.5 years before. The 0.1--10 keV spectrum is described by an absorbed power-law continuum with photon-number spectral index Gamma=1.8+-0.2 and hydrogen column density of NH=1.0^{+0.4}_{-0.3} x 10^{22} cm^{-2}. According to the orbital parameters of the system the BeppoSAX observation covers the time of an X-ray eclipse should one occur. However, the 1.6-10 keV light curve does not show evidence for such an event, which allows us to give an upper limit to the inclination of the system. The low X-ray flux detected during this observation is interpreted as a decrease in the mass accretion rate onto the compact object due to a decrease in the mass-loss rate from the primary.Comment: 6 pages, 5 figures, accepted for publication in A&

The Spectroscopic Orbit of the Evolved Binary HD 197770

We have used spectra taken between 1992 and 1997 to derive the spectroscopic orbit of the eclipsing double-lined spectroscopic binary HD 197770. This binary has a period of 99.69 +/- 0.02 days and K amplitudes of 31.2 +/- 0.8 and 47.1 +/- 0.4 km s^{-1} for components A & B, respectively. The msin^{3}i values for A & B are 2.9 and 1.9, respectively, and are close to the actual masses due to the eclipsing nature of this binary. Both components of HD 197770 have spectral types near B2 III. This means both components are undermassive by about a factor of five and, thus, evolved stars. Additional evidence of the evolved nature of HD 197770 is found in 25, 60, and 100 micron IRAS images of HD 197770. These images show 2 apparent shells centered on HD 197770; a bright 60 micron shell with a 14' diameter and a larger (1.2 degeree diameter) bubble-like feature. At least one of the components of HD 197770 is likely to be a post-AGB star.Comment: will be published in the AJ (1998 June), also availible at http://snake.phys.lsu.edu/~gordon/papers/hd197770.htm

Faint Supernovae and Supernova Impostors: Case studies of SN2002kg/NGC2403-V37 and SN 2003gm

Photometric and spectroscopic observations of the faint Supernovae (SNe) 2002kg and 2003gm, and their precursors, in NGC 2403 and NGC 5334 respectively, are presented. The properties of these SNe are discussed in the context of previously proposed scenarios for faint SNe: low mass progenitors producing under-energetic SNe; SNe with ejecta constrained by a circumstellar medium; and outbursts of massive Luminous Blue Variables (LBVs). The last scenario has been referred to as ``Type V SNe'', ``SN impostors'' or ``fake SNe.'' The faint SN 2002kg reached a maximum brightness of $\mathrm{M_{V}=-9.6}$, much fainter than normal type II SNe. The precursor of SN 2002kg is confirmed to be, as shown in previous work, the LBV NGC2403-V37. Strong $\mathrm{Fe II}$ lines are observed in the spectra of SN 2002kg, similar to both the LBV NGC2363-V1 and the type IIn SN 1995G. The spectrum of SN 2002kg does show strong resolved $\mathrm{[N II]}$ at $\lambda\lambda$6549,6583\ang. The identified progenitor of SN 2003gm is a bright yellow star, consistent with a F5-G2 supergiant. SN 2003gm, at the epoch of discovery, was of similar brightness to the possible fake SN 1997bs. Photometrically SN 2003gm shows the same decrease in brightness, over the same time period as SN 1997bs. The early time spectra of SN 2003gm are dominated by Balmer emission lines, which at the observed resolution, appear similar to SN 2000ch. On the basis of the post-discovery photometric and spectroscopic observations presented here we suggest that SN 2003gm is a similar event to SN 1997bs. The presence of strong $\mathrm{[N II]}$ lines, near $\mathrm{H\alpha}$, is suggested as a possible means of identifying objects such as SN 2002kg/NGC2403-V37 as being LBVs - although not as a general classification criterion of all LBVs masquerading as SNe (abridged).Comment: 17 pages, 17 figures (4 jpg), MNRAS accepted, 4 typos correcte

A Study of the Type II-P Supernova 2003gd in M74

We present photometric and spectroscopic data of the type II-P supernova 2003gd, which was discovered in M74 close to the end of its plateau phase. SN 2003gd is the first type II supernova to have a directly confirmed red supergiant progenitor. We compare SN 2003gd with SN 1999em, a similar type II-P supernova, and estimate an explosion date of 18th March 2003. We determine a reddening towards the supernova of E(B-V) = 0.14+/-0.06, using three different methods. We also calculate three new distances to M74 of 9.6+/-2.8 Mpc, 7.7+/-1.7 Mpc and 9.6+/-2.2 Mpc. The former was estimated using the Standardised Candle Method (SCM), for type II supernovae, and the latter two using the Brightest Supergiants Method (BSM). When combined with existing kinematic and BSM distance estimates, we derive a mean value of 9.3+/-1.8 Mpc. SN 2003gd was found to have a lower tail luminosity compared to other ``normal'' type II-P SNe bringing into question the nature of this supernova. We present a discussion concluding that this is a ``normal'' type II-P supernova which is consistent with the observed progenitor mass of 8(+4/-2) Mo.Comment: 23 pages, 24 figures to appear in MNRA

Spring temperatures influence selection on breeding date and the potential for phenological mismatch in a migratory bird

Climate change has affected the seasonal phenology of a variety of taxa, including that of migratory birds and their critical food resources. However, whether climate-induced changes in breeding phenology affect individual fitness, and how these changes might, therefore, influence selection on breeding date remain unresolved. Here, we use a 36-year dataset from a long-term, individual-based study of House Wrens (Troglodytes aedon) to test whether the timing of avian breeding seasons is associated with annual changes in temperature, which have increased to a small but significant extent locally since the onset of the study in 1980. Increasing temperature was associated with an advancement of breeding date in the population, as the onset of breeding within years was closely associated with daily spring temperatures. Warmer springs were also associated with a reduced incubation period, but reduced incubation periods were associated with a prolonged duration of nestling provisioning. Nest productivity, in terms of fledgling production, was not associated with temperature, but wetter springs reduced fledging success. Most years were characterized by selection for earlier breeding, but cool and wet years resulted in stabilizing selection on breeding date. Our results indicate that climate change and increasing spring temperatures can affect suites of life-history traits, including selection on breeding date. Increasing temperatures may favor earlier breeding, but the extent to which the phenology of populations might advance may be constrained by reductions in fitness associated with early breeding during cool, wet years. Variability in climatic conditions will, therefore, shape the extent to which seasonal organisms can respond to changes in their environment.Peer reviewedIntegrative Biolog

On the radio emitting high mass X-ray binary LS 5039

We present new optical - near-IR spectroscopic and photometric observations of the newly discovered galactic microquasar LS 5039, which indicate a classification for the mass donor in the system of O6.5V((f)). Optical spectroscopy and photometry shows no variability over a timescale of years, and we find no evidence of modulation by, or emission from the compact companion in these data. However significant photometric variability (~0.4 mag) is present in the H and K bands between 1995-2000. Such variability has been observed in other radio bright X-ray binaries where it has been attributed to synchrotron emission from the jet. However, given the non-thermal spectral index of the radio emission in LS 5039 this explanation appears unlikely, predicting a near-IR flux ~3 orders of magnitude too small to contribute significantly at such wavelengths. Nightly optical photometry over a 21 day period between 2000 May-June reveals variability at a level of a few hundredths of a magnitude, with no periodicity or long term trend visible. Likewise, while the radio lightcurves show moderate variability ( per cent of the mean flux density) we find no evidence of periodic modulation - Monte Carlo simulations constrain any such periodic variability to <4 per cent modulation at 2.25 GHz. The differences in behaviour between LS 5039 and Cygnus X-1 - the most closely related radio emitting High Mass X-ray Binary - are likely to be a result of the weaker stellar wind and probable greater orbital separation of LS 5039 compared to Cyg X-1