Elucidation of the transmission patterns of an insect-borne bacterium

Quantitative data on modes of transmission are a crucial element in understanding the ecology of microorganisms associated with animals. We investigated the transmission patterns of a -proteobacterium informally known as pea aphid Bemisia-like symbiont (PABS), also known as T-type, which is widely but not universally distributed in natural populations of the pea aphid, Acyrthosiphon pisum. The vertical transmission of PABS to asexual and sexual morphs and sexually produced eggs was demonstrated by a diagnostic PCR-based assay, and the maximum estimated failure rate was 2%. Aphids naturally lacking PABS acquired PABS bacteria administered via the diet, and the infection persisted by vertical transmission for at least three aphid generations. PABS was also detected in two of five aphid honeydew samples tested and in all five siphuncular fluid samples tested but in none of 15 samples of salivary secretions from PABS-positive aphids. However, PABSnegative aphids did not acquire PABS when they were cocultured with PABS-positive aphids; the maximal estimated level of horizontal transmission was 18%. A deterministic model indicated that the force of infection by a horizontal transmission rate of 3% is sufficient to maintain a previously described estimate of the prevalence of PABS-positive aphids (37%), if the vertical transmission rate is 98%. We concluded that PABS infections in A. pisum can be maintained by high vertical transmission rates and occasional horizontal transmission, possibly via the oral route, in the absence of selection either for or against aphids bearing this bacterium

Stories of success: Cultural narratives and personal stories of elite and professional athletes

Using a narrative methodology to explore the stories Olympic and elite athletes tell about success, we identified three alternatives to the dominant conception of success as the achievement of performance outcomes. In these alternatives, success is storied as: (1) ‘I did the best that I could’ – a controllable and sustainable story of effort and application; (2) ‘It’s the closest thing you can get to flying’ – a story where success relates to embodied experience and discovery; (3) ‘People I made the journey with’ – which prioritises relationships and connection between people. We reflect on three key insights: (1) success is a multidimensional concept, broader than the singular conception encapsulated within the dominant performance narrative; (2) through various narrative strategies, experienced athletes resist cultural pressures towards a singular conception of success; (3) for long-term performance and well-being, it is necessary to work towards multiple forms of success over time and across contexts

We haven't got a seat on the bus for you or All the seats are mine: Narratives and career transitions in professional golf

In this article we explore how the stories an athlete tells throughout life in sport affect her career transition experiences. We base our enquiry on a social constructionist conception of narrative theory which holds that storytelling is integral to the creation and maintenance of identity and sense of self. Life stories were gathered through interviews with two professional women golfers (Christiana and Kandy) over a six‐year period. Through a narrative analysis of structure and form we explored each participant’s stories of living in and withdrawing from professional golf. We suggest Christiana told monological performance‐oriented stories which, while aligning with the culture of elite sport, resulted in an exclusive athletic identity and foreclosure of alternative selves and roles. On withdrawal, Christiana experienced narrative wreckage, identity collapse, mental health difficulties and considerable psychological trauma. In contrast, Kandy told dialogical discovery‐oriented stories which, while being in tension with the dominant performance narrative, created and sustained a multidimensional identity and self. Her stories and identity remained intact, authentic and continuous on withdrawal from tournament golf and she experienced few psychological problems

Axons in Cat Visual Cortex are Topologically Self-similar

The axonal arbors of the different types of neocortical and thalamic neurons appear highly dissimilar when viewed in conventional 2D reconstructions. Nevertheless, we have found that their one-dimensional metrics and topologies are surprisingly similar. To discover this, we analysed the axonal branching pattern of 39 neurons (23 spiny, 13 smooth and three thalamic axons) that were filled intracellularly with horseradish peroxidase (HRP) during in vivo experiments in cat area 17. The axons were completely reconstructed and translated into dendrograms. Topological, fractal and Horton-Strahler analyses indicated that axons of smooth and spiny neurons had similar complexity, length ratios (a measure of the relative increase in the length of collateral segments as the axon branches) and bifurcation ratios (a measure of the relative increase in the number of collateral segments as the axon branches). We show that a simple random branching model (Galton-Watson process) predicts with reasonable accuracy the bifurcation ratio, length ratio and collateral length distribution of the axonal arbor

Biological control of an agricultural pest protects tropical forests

Though often perceived as an environmentally-risky practice, biological control of invasive species can restore crop yields, ease land pressure and thus contribute to forest conservation. Here, we show how biological control against the mealybug Phenacoccus manihoti (Hemiptera) slows deforestation across Southeast Asia. In Thailand, this newly-arrived mealybug caused an 18% decline in cassava yields over 2009–2010 and an escalation in prices of cassava products. This spurred an expansion of cassava cropping in neighboring countries from 713,000 ha in 2009 to > 1 million ha by 2011: satellite imagery reveals 388%, 330%, 185% and 608% increases in peak deforestation rates in Cambodia, Lao PDR, Myanmar and Vietnam focused in cassava crop expansion areas. Following release of the host-specific parasitoid Anagyrus lopezi (Hymenoptera) in 2010, mealybug outbreaks were reduced, cropping area contracted and deforestation slowed by 31–95% in individual countries. Hence, when judiciously implemented, insect biological control can deliver substantial environmental benefits

Using Stories in Coach Education

The purpose of this paper is to illustrate how storied representations of research can be used as an effective pedagogical tool in coach education. During a series of continuing professional development seminars for professional golf coaches, we presented our research in the form of stories and poems which were created in an effort to evoke and communicate the lived experiences of elite professional golfers. Following these presentations, we obtained written responses to the stories from 53 experienced coaches who attended the seminars. Analysis of this data revealed three ways in which coaches responded to the stories: (i) questioning; (ii) summarising; and (iii) incorporating. We conclude that these responses illustrate the potential of storied forms of representation to enhance professional development through stimulating reflective practice and increasing understanding of holistic, person-centred approaches to coaching athletes in high-performance sport

Product CFTs, gravitational cloning, massive gravitons and the space of gravitational duals

The question of graviton cloning in the context of the bulk/boundary correspondence is considered. It is shown that multi-graviton theories can be obtained from products of large-N CFTs. No more than one interacting massless graviton is possible. There can be however, many interacting massive gravitons. This is achieved by coupling CFTs via multi-trace marginal or relevant perturbations. The geometrical structure of the gravitational duals of such theories is that of product manifolds with their boundaries identified. The calculational formalism is described and the interpretation of such theories is discussed.Comment: Latex, 25 pages. (v2) Minor corrections and references adde

‘It stays with you’: multiple evocative representations of dance and future possibilities for studies in sport and physical cultures

This article considers the integration of arts-based representations via poetic narratives together with artistic representation on dancing embodiment so as to continue an engagement with debates regarding multiple forms/representations. Like poetry, visual images are unique and can evoke particular kinds of emotional and visceral responses, meaning that alternative representational forms can resonate in different and powerful ways. In the article, we draw on grandparent-grandchild interactions, narrative poetry, and artistic representations of dance in order to illustrate how arts-based methods might synergise to offer new ways of ‘knowing’ and ‘seeing’. The expansion of the visual arts into interdisciplinary methodological innovations is a relatively new, and sometimes contentious approach, in studies of sport and exercise. We raise concerns regarding the future for more arts-based research in the light of an ever-changing landscape of a neoliberal university culture that demands high productivity in reductionist terms of what counts as ‘output’, often within very restricted time-frames. Heeding feminist calls for ‘slow academies’ that attempt to ‘change’ time collectively, and challenge the demands of a fast-paced audit culture, we consider why it is worth enabling creative and arts-based methods to continue to develop and flourish in studies of sport, exercise and health, despite the mounting pressures to ‘perform’

Metabolomic Evidence for a Field Effect in Histologically Normal and Metaplastic Tissues in Patients with Esophageal Adenocarcinoma

Patients with Barrett's esophagus (BO) are at increased risk of developing esophageal adenocarcinoma (EAC). Most Barrett's patients, however, do not develop EAC, and there is a need for markers that can identify those most at risk. This study aimed to see if a metabolic signature associated with the development of EAC existed. For this, tissue extracts from patients with EAC, BO, and normal esophagus were analyzed using 1H nuclear magnetic resonance. Where possible, adjacent histologically normal tissues were sampled in those with EAC and BO. The study included 46 patients with EAC, 7 patients with BO, and 68 controls who underwent endoscopy for dyspeptic symptoms with normal appearances. Within the cancer cohort, 9 patients had nonneoplastic Barrett's adjacent to the cancer suitable for biopsy. It was possible to distinguish between histologically normal, BO, and EAC tissue in EAC patients [area under the receiver operator curve (AUROC) 1.00, 0.86, and 0.91] and between histologically benign BO in the presence and absence of EAC (AUROC 0.79). In both these cases, sample numbers limited the power of the models. Comparison of histologically normal tissue proximal to EAC versus that from controls (AUROC 1.00) suggests a strong field effect which may develop prior to overt EAC and hence be useful for identifying patients at high risk of developing EAC. Excellent sensitivity and specificity were found for this model to distinguish histologically normal squamous esophageal mucosa in EAC patients and healthy controls, with 8 metabolites being very significantly altered. This may have potential diagnostic value if a molecular signature can detect tissue from which neoplasms subsequently arise

Topological Orthoalgebras

We define topological orthoalgebras (TOAs) and study their properties. While every topological orthomodular lattice is a TOA, the lattice of projections of a Hilbert space is an example of a lattice-ordered TOA that is not a toplogical lattice. On the other hand, we show that every compact Boolean TOA is a topological Boolean algebra. We also show that a compact TOA in which 0 is an isolated point is atomic and of finite height. We identify and study a particularly tractable class of TOAs, which we call {\em stably ordered}: those in which the upper-set generated by an open set is open. This includes all topological OMLs, and also the projection lattices of Hilbert spaces. Finally, we obtain a topological version of the Foulis-Randall representation theory for stably ordered TOAsComment: 16 pp, LaTex. Minor changes and corrections in sections 1; more substantial corrections in section