Ambassadør for offentlig ledelse – hvad er det for noget?

Hvervet som ledelsesambassadør udspringer af aftalen om ledelse og kompetencer, som den tidligere Regering, KL og Danske Regioner indgik i februar 2019. Blandt de mange initiativer i aftalen er en årlig udnævnelse af en ambassadør for offentlig ledelse, og jeg var så heldig at blive udpeget som de første ledelsesambassadør. I denne artikel vil jeg komme ind på, hvad jeg synes er vigtigt at sætte fokus på som offentlig leder her i 2020

A two-dimensional gel protein database of noncultured total normal human epidermal keratinocytes : identification of proteins strongly up-regulated in psoriatic epidermis

A two-dimensional (2-D) gel database of proteins from noncultured total normal human epidermal keratinocytes has been established. A total of 1449 [35S]methionine labelled proteins (1112 isoelectric focusing, 337 nonequilibrium pH gradient electrophoresis) were resolved and recorded using computer assisted (PDQ-SCAN and PDQUEST software) 2-D gel electrophoresis. By matching the protein patterns of total keratinocytes and transformed human amnion cells (master database; Celis et al., Leukemia 1988, 2, 561-602) as well as by 2-D immunoblotting and microsequencing of keratinocyte proteins, it was possible to identify 72 polypeptides in the keratinocyte database. The database also includes data on polypeptides that are synthesized at a higher level by keratinocytes enriched in basal cells, and on six secreted proteins which are produced, albeit at a reduced rate, by normal keratinocytes and that are strongly up-regulated in psoriatic epidermis (Celis et al., FEBS Letters, in press)

Co-segregation analysis of a <i>BAP1</i> splice mutation in a Danish family.

<p>In the pedigree individuals that have uveal melanoma (UMM) are represented by black circles (female) or boxes (male) and individuals with cutaneous melanoma (CMM) are indicated by grey circles or boxes. The age of diagnosis of each melanoma is indicated in brackets. A line through a symbol indicates that the person is now deceased. If a person carries the <i>BAP1</i> splice mutation it is indicated by an ‘M’ and if they are wild type for this variant it is indicated with a ‘WT’. Where the mutation status is indicated in brackets, the person is a presumed obligate carrier, ‘(M)’. Other cancer types are also indicated in the pedigree with the age of diagnosis in brackets. Asterisks indicate the two individuals that were exome sequenced. Unaffected siblings are represented by diamonds, with the number in the centre indicating the number of people.</p

Sanger sequencing trace and amino acid alignment showing the truncated BAP1 protein.

<p>(A) The left panel shows the wild type chromatogram while the right panel shows that of the <i>BAP1</i> splice mutation. (B) Wild type nucleotide and amino acid sequences are shown in the upper panel while the lower panel shows part of the truncated BAP1 protein resulting from the loss of exon 8.</p