5 research outputs found
Effet de lâapplication du fongicide sur la productivitĂ© de lâeau du blĂ© tendre
En Tunisie, le secteur cĂ©rĂ©alier joue un rĂŽle socio-Ă©conomique important. Le prĂ©sent travail est rĂ©alisĂ© dans lâobjectif dâĂ©tudier lâeffet dâun fongicide sur lâefficience de lâutilisation de lâeau des anciennes variĂ©tĂ©s et des nouvelles obtentions de blĂ© tendre. Les composantes finales du rendement ainsi que les efficiences de lâutilisation de lâeau des anciennes et nouvelles variĂ©tĂ©s ont Ă©tĂ© statistiquement analysĂ©es Ă la fin de la compagne expĂ©rimentale. Les rĂ©sultats ont montrĂ© que les variĂ©tĂ©s Kodss et Maktarus ont prĂ©sentĂ© des performances meilleures mĂȘme en absence du traitement fongicide. Ceci confirme leurs capacitĂ©s de rĂ©sistance Ă la rouille jaune la plus dĂ©vastatrice de la culture du blĂ© tendre. NĂ©anmoins, le fongicide utilisĂ© (ogame) a prouvĂ© son efficacitĂ© Ă protĂ©ger les cultures contre les stress biotiques et Ă amĂ©liorer les rendements et la qualitĂ© de la production. Ainsi, il est recommandĂ© dâĂ©viter les traitements fongiques sur les nouvelles variĂ©tĂ©s
Study of Coxsackie B viruses interactions with Coxsackie Adenovirus receptor and Decay-Accelerating Factor using Human CaCo-2 cell line
Effet de lâapplication du fongicide sur la productivitĂ© de lâeau du blĂ© tendre
En Tunisie, le secteur cĂ©rĂ©alier joue un rĂŽle socio-Ă©conomique important. Le prĂ©sent travail est rĂ©alisĂ© dans lâobjectif dâĂ©tudier lâeffet dâun fongicide sur lâefficience de lâutilisation de lâeau des anciennes variĂ©tĂ©s et des nouvelles obtentions de blĂ© tendre. Les composantes finales du rendement ainsi que les efficiences de lâutilisation de lâeau des anciennes et nouvelles variĂ©tĂ©s ont Ă©tĂ© statistiquement analysĂ©es Ă la fin de la compagne expĂ©rimentale. Les rĂ©sultats ont montrĂ© que les variĂ©tĂ©s Kodss et Maktarus ont prĂ©sentĂ© des performances meilleures mĂȘme en absence du traitement fongicide. Ceci confirme leurs capacitĂ©s de rĂ©sistance Ă la rouille jaune la plus dĂ©vastatrice de la culture du blĂ© tendre. NĂ©anmoins, le fongicide utilisĂ© (ogame) a prouvĂ© son efficacitĂ© Ă protĂ©ger les cultures contre les stress biotiques et Ă amĂ©liorer les rendements et la qualitĂ© de la production. Ainsi, il est recommandĂ© dâĂ©viter les traitements fongiques sur les nouvelles variĂ©tĂ©s
Natural Recombination Event within the Capsid Genomic Region Leading to a Chimeric Strain of Human Enterovirus Bâż
Recombination between two strains is a known phenomenon for enteroviruses replicating within a single cell. We describe a recombinant strain recovered from human stools, typed as coxsackievirus B4 (CV-B4) and CV-B3 after partial sequencing of the VP1 and VP2 coding regions, respectively. The strain was neutralized by a polyclonal CV-B3-specific antiserum but not by a CV-B4-specific antiserum. The nucleotide sequence analysis of the whole structural genomic region showed the occurrence of a recombination event at position 1950 within the VP3 capsid gene, in a region coding for the 2b antigenic site previously described for CV-B3. This observation evidences for the first time the occurrence of an interserotypic recombination within the VP2-VP3-VP1 capsid region between two nonpoliovirus enterovirus strains. The neutralization pattern suggests that the major antigenic site is located within the VP2 protein
Typing of Human Enterovirus by Partial Sequencing of VP2âż
The sequencing of the VP1 hypervariable region of the human enterovirus (HEV) genome has become the reference test for typing field isolates. This study describes a new strategy for typing HEV at the serotype level that uses a reverse transcription-PCR assay targeting the central part of the VP2 capsid protein. Two pairs of primers were used to amplify a fragment of 584 bp (with reference to the PV-1 sequence) or a part of it (368 bp) for typing. For a few strains not amplified by the first PCR, seminested primers enhanced the sensitivity (which was found to be approximately 10â1 and 10â4 50% tissue culture infective dose per reaction tube for the first and seminested assay, respectively). The typing method was then applied to 116 clinical and environmental strains of HEV. Sixty-one typeable isolates were correctly identified at the serotype level by comparison to seroneutralization. Forty-eight of 55 âuntypeableâ strains (87.3%) exhibited the same serotype using VP1 and VP2 sequencing methods. For six strains (four identified as EV-71, one as E-9, and one as E-30 by the VP2 method), no amplification was obtained by the VP1 method. The last strain, typed as CV-B4 by VP1 and CV-B3 by VP2 and monovalent antiserum, could exhibit recombination within the capsid region. Although the VP2 method was tested on only 36 of the 68 HEV serotypes, it appears to be a promising strategy for typing HEV strains isolated on a routine basis. The good sensitivity of the seminested technique could avoid cell culture and allow HEV typing directly from PCR products