The Result of Some Biomarker of Free Radical-Peroxidation of Lipids and Antioxidant System of Experimental Acute Infarction Model Induced by Coronary Artery Occlusion in Rats

Background: Recently years, Increasing coronary artery disease, diabetes, and cancer in the worldwide. Epidemiological studies indicate that ischemic heart disease will constitute the major disease-burden worldwide by the year 2020. Myocardial infarction is invariably followed by numerous pathophysiological and biochemical alterations including hyperlipidemia, thrombosis, lipid peroxidation and free radical damage etc., leading to qualitative and quantitative changes of myocardium. Determination of in blood serum and cardiac tissue homogenate levels of cardiac biomarker cardiac specific troponin I and estimation of lipid peroxidation products and some antioxidants enzymes of experimental acute infarction model induced by coronary artery occlusion in rats. Materials and methods: Adult male Wistar rats, weighting approximately 180 to 200±20 gram, used for the experiment. The were divided randomly into 2 groups (6-8 animals in each group). They were distributed as follow: first group (healthy, non treatment), second group (control, experimental acute infarction model induced by coronary artery occlusion in rats,  non treatment). We did coronary occlusion induced myocardial ischemia by Kogan A.K., Ambaga M /1979/‘s method.The experiment is 1st, 3rd, 7th, 14th 21st days blood samples were collected and used to determine in blood serum and cardiac tissue homogenate levels of cardiac biomarker cardiac specific troponin I (CTn-I) and estimation of lipid peroxidation products (MDA, SA, LPO) and some antioxidants enzymes (SOD, GSH, GSH-px) were estimated using standard rat ELISA KIT by enzyme-linked immunesorbent assay. Result: Determination of cardiac troponin-I levels in blood serum of experimental acute infarction model induced by coronary artery occlusion in rats increased by 31.9-58.4% in the 1st, 3rd, 7th, 14th 21st days of the test compared to healthy groups. The cardiac biomarker cardiac specific troponin I is indicative for cardiomyocyte damage and is currently used in the diagnosis and prognosis of myocardial ischemia. Experimental acute infarction model induced by coronary artery occlusion in white rats, it is sufficiently shown that from day 1-21 MDA in plasma increased by 43.9 – 76.3%, plasma SA by 18.3-49.8% and LPO by 12.8-38.3%. This results in necrosis of cells and tissues, dissolving of membrane and stimulation of pathogenesis of disruption of membrane parts. It is also shown that substances such as endogen and antioxidant decreases in heart ischemia. Conclusion: Determination of increased level are lipid peroxidation products (MDA, SA, LPO, CTn-I), and decreased level are antioxidants (SOD, GSH, GSH-px) in blood serum of experimental acute infarction model induced by coronary artery occlusion in rats, it seems to induced of pathogenesis of coronary disease and infarction myocardium while the accumulation of lipid product cause to damage the cell membrane

A study on structure and functions of organs involved in the formation of Mongolian Khuumii sound

Khuumii (throat or overtone singing) is a unique form of art derived from the nomadic population of Central Asia, which is a type of singing in which the singer manipulates the resonances (or formants) created as air travels from the lungs, past the vocal folds, and out of the lips to produce a melody. A total of 60 participants, aged 18-60 years (54 men and 6 women), were selected by non-random sampling method using cross-sectional study. X-ray, endoscopy, and sound research method were used in the study, and the composition of blood gas was analysed. X-ray examination determined the state during each different types of Khuumii; Shakhaa and Kharkhiraa. As the basic timbre of Shakhaa Khuumii went up progressively the larynx grew and the compression strength increased, while the basic timbre went down, and the larynx became lower. In the case of Kharkhiraa Khuumii, the larynx position was elevated to a relatively small extent compared to Shakhaa Khuumii and the distance between the sublingual bone and the larynx was large. The sublingual bone trunk lowered during Shakhaa Khuumii, while it was slightly elevated during Kharkhiraa Khuumii. The laryngeal endoscopy evaluated the movement of true and false vocal chords, glottal volume, movements of epiglottis and arytenoid cartilage, and mucosa. Furthermore, the sound frequency is 2-4 times higher than that of normal speech, and sound volume is 0.5-1 times higher. The blood gas composition test showed partial pressure (pO2), and saturation of oxygen (SaO2) decreased after performing Khuumii. In the case of Shakhaa and Kharkhiraa Khuumi, it is appropriate to divide Khuumii into two main types according to structural and functional changes in the organs involved