A Single-Layer Multimode Metasurface Antenna with a CPW-Fed Aperture for UWB Communication Applications

A single-layer multimode metasurface antenna is proposed with a coplanar waveguide (CPW)-fed aperture. The ultra-wideband (UWB) performance is implemented based on a three-step evolution process with the aid of characteristic mode analysis (CMA). Considering the efficient excitation with a fixed feeding structure, the metasurface modal current variation at different frequencies is analyzed and optimized, in addition to that at the resonant frequency. Correspondingly, the metasurface is firstly designed utilizing an array of 4 × 4 patches. Then, the 1 × 3 and the 1 × 1 parasitic patch arrays are located near the edge patches. Finally, every patch is split into two by a center slot along the current distribution of the required polarization. Four resonant modes of the metasurface become more desirable step by step and can be efficiently excited over the entire band. To enhance the impedance matching level, a pair of 5-stage gradient transitions are added to the CPW-fed slot. The slot mode combined with the four modes further improves the bandwidth. The experimental results demonstrate that the proposed antenna exhibits a 3 dB gain bandwidth of over 74% (4.0–8.7 GHz) with a peak gain of 8.2 dBi. The overall dimensions of the prototype are 1.40λ0 × 1.40λ0 × 0.075λ0 (λ0 is the free-space wavelength at 6 GHz)

Improvement of CT Target Scanning Quality for Pulmonary Nodules by PDCA Management Method

High CT image quality is an important guarantee for doctors to correctly diagnose pulmonary nodules. The aim of this study was to explore the application value of PDCA management method in improving the quality of CT target scanning for pulmonary nodules. We identified 480 patients’ CT image with at least one pulmonary nodule admitted in Ninghai First hospital from September 1st, 2018, to April 30th, 2019. 240 CT images are carried out by the conventional target scanning method, and we analyzed the reasons for the low quality of some CT target scanning images of pulmonary nodules in the radiology department of our hospital. We established a new process of CT target scanning for pulmonary nodules based on the PDCA method and then tested 240 patients who were checked after January 1st, 2019. The excellent rate of CT target scanning image of pulmonary nodules in our department increased from 60.0% to more than 90.0%. The patients’ satisfaction with the examination was significantly higher than that without the implementation of PDCA management. The research result indicated that the process of CT target scanning image, postprocessing reconstruction, and numerical measurement of pulmonary nodules can be improved by standardized PDCA cycle, which benefits effectively improving the theoretical and operational skills of radiologists and significantly improving the image quality rate of CT target scanning of pulmonary nodules

Molecular crosstalk between articular cartilage, meniscus, synovium, and subchondral bone in osteoarthritis

AimsOsteoarthritis (OA) is a common degenerative joint disease worldwide, which is characterized by articular cartilage lesions. With more understanding of the disease, OA is considered to be a disorder of the whole joint. However, molecular communication within and between tissues during the disease process is still unclear. In this study, we used transcriptome data to reveal crosstalk between different tissues in OA.MethodsWe used four groups of transcription profiles acquired from the Gene Expression Omnibus database, including articular cartilage, meniscus, synovium, and subchondral bone, to screen differentially expressed genes during OA. Potential crosstalk between tissues was depicted by ligand-receptor pairs.ResultsDuring OA, there were 626, 97, 1,060, and 2,330 differentially expressed genes in articular cartilage, meniscus, synovium, and subchondral bone, respectively. Gene Ontology enrichment revealed that these genes were enriched in extracellular matrix and structure organization, ossification, neutrophil degranulation, and activation at different degrees. Through ligand-receptor pairing and proteome of OA synovial fluid, we predicted ligand-receptor interactions and constructed a crosstalk atlas of the whole joint. Several interactions were reproduced by transwell experiment in chondrocytes and synovial cells, including TNC-NT5E, TNC-SDC4, FN1-ITGA5, and FN1-NT5E. After lipopolysaccharide (LPS) or interleukin (IL)-1β stimulation, the ligand expression of chondrocytes and synovial cells was upregulated, and corresponding receptors of co-culture cells were also upregulated.ConclusionEach tissue displayed a different expression pattern in transcriptome, demonstrating their specific roles in OA. We highlighted tissue molecular crosstalk through ligand-receptor pairs in OA pathophysiology, and generated a crosstalk atlas. Strategies to interfere with these candidate ligands and receptors may help to discover molecular targets for future OA therapy.Cite this article: Bone Joint Res 2022;11(12):862–872

Blocking TRPV4 Ameliorates Osteoarthritis by Inhibiting M1 Macrophage Polarization via the ROS/NLRP3 Signaling Pathway

Osteoarthritis (OA) is a low-level inflammatory disease in which synovial macrophage M1 polarization exacerbates the progression of synovitis and OA. Notedly, the ROS (reactive oxygen species) level in macrophages is intimately implicated in macrophage M1 polarization. TRPV4 (transient receptor potential channel subfamily V member 4), as an ion channel, plays a pivotal role in oxidative stress and inflammation. In this study, we investigated the role of TRPV4 in OA progression and M1 macrophage polarization. Male adult Sprague–Dawley (SD) rats underwent a medial meniscus radial transection operation to create an OA model in vivo and RAW 264.7 cells were intervened with 100 ng/mL LPS (lipopolysaccharide) to induce M1-polarized macrophages in vitro. We demonstrated that the infiltration of M1 synovial macrophages and the expression of TRPV4 were increased significantly in OA synovium. In addition, intra-articular injection of HC067074 (a specific inhibitor of TRPV4) alleviated the progression of rat OA and significantly decreased synovial macrophage M1 polarization. Further mechanisms suggested that ROS production by M1 macrophages was decreased after TRPV4 inhibition. In addition, NLRP3 (pyrin domain containing protein 3) as a downstream effector of ROS in M1-polarized macrophage, was significantly suppressed following TRPV4 inhibition. In conclusion, this study discovered that inhibition of TRPV4 delays OA progression by inhibiting M1 synovial macrophage polarization through the ROS/NLRP3 pathway

Niobium carbide (MXene) reduces UHMWPE particle-induced osteolysis

Joint replacement surgery is one of the orthopedic surgeries with high successful rates; however, wear debris generated from prostheses can ultimately lead to periprosthetic osteolysis and failure of the implant. The implant-derived particulate debris such as ultrahigh molecular weight polyethylene (UHMWPE) can initiate the local immune response and recruit monocytic cells to phagocytose particles for generating reactive oxygen species (ROS). ROS induces osteoclastogenesis and macrophages to secrete cytokines which ultimately promote the development of osteolysis. In this work, we develop the few-layered Nb2C (FNC) as an antioxidant which possesses the feature of decreasing the production of cytokines and inhibiting osteoclastogenesis by its ROS adsorption. Moreover, local injection of FNC attenuates the UHMWPE-induced osteolysis in a mouse calvarial model. In sum, our results suggest that FNC can be used for treating osteolytic bone disease caused by excessive osteoclastogenesis