114 research outputs found
Anti-dendriform algebras, new splitting of operations and Novikov type algebras
We introduce the notion of anti-dendriform algebras as a new approach of
splitting the associativity. They are characterized as the algebras with two
operations whose sum is associative and the negative left and right
multiplication operators compose the bimodules of the sum associative algebras,
justifying the notion due to the comparison with the corresponding
characterization of dendriform algebras. The notions of anti--operators and anti-Rota-Baxter operators on associative algebras are
introduced to interpret anti-dendriform algebras. In particular, there are
compatible anti-dendriform algebra structures on associative algebras with
nondegenerate commutative Connes cocycles. There is an important observation
that there are correspondences between certain subclasses of dendriform and
anti-dendriform algebras in terms of -algebras. As a direct consequence, we
give the notion of Novikov-type dendriform algebras as an analogue of Novikov
algebras for dendriform algebras, whose relationship with Novikov algebras is
consistent with the one between dendriform and pre-Lie algebras. Finally we
extend to provide a general framework of introducing the notions of analogues
of anti-dendriform algebras, which interprets a new splitting of operations.Comment: 25 page
An extracellular protein expression system in Escherichia coli implies potential application
Escherichia coli is commonly used as a host for the extracellular production of proteins. However, its secretion capacity is often limited to a frustratingly low level compared with other expression hosts, because E. coli has a complex cell envelope with two layers. We recently identified the catalytic domain of a cellulase (Cel-CD) from Bacillus sp. that can be secreted into the medium from recombinant E. coli in large quantities without its native signal peptide. By subcellular location analysis, we verified that the secretion was a two-step process via the SecB-dependent pathway through the inner membrane and an unknown pathway through the outer membrane. However, the N-terminal region of Cel-CD is polar and hydrophilic, which showed no similarities to other typical signal sequences. Random mutagenesis experiment suggested that the N-terminal sequence is a compromising result of transportation through inner and outer membranes. This is the first report that a non-classical signal peptide can guide recombinant proteins out of the cells from cytoplasm. Both the Cel-CD and its N-terminal sequence can serve as carriers for efficient extracellular production of select target proteins with a concentration from 101 to 691 mg/L in flask cultivation.
This protein can degrading cellulose efficiently in the culture medium indicating a great potential. Therefore, a recombinant E.coli that can directly utilize cellulose as sole carbon source by fusion Cel-CD with a b-glucosidase was constructed. Recombinant strains were confirmed to use the amorphous cellulose as well as cellobiose as the sole carbon source for growth. Furthermore, both strains were engineered with poly (3-hydroxybutyrate) (PHB) synthesis pathway to demonstrate the production of biodegradable polyesters directly from cellulose materials without exogenously added cellulases. The results suggested that this system has a potential application in lignocellulosic biomass degradation and biochemical biofuel production.
These guidelines have been prepared in the format that should be used for the abstract submission. Authors should replace the text of this template in order to prepare their abstracts. Fonts, sizes and spacing should be used as they are used in this document. Page size is US 8.5 inch x 11 inch, top and bottom margin 0.8 inches, left and right margin 0.8 inches. Body text should be written in Arial, 10 pt, single spacing. The Abstract, in English, should introduce the proposed paper’s subject, summarize its contents, explain any unique aspects, and clearly indicate the specific relevance to the themes of the Conference. Do not sub-divide the text into separate sections. References may be included at the bottom.
Reference Exploring the N-terminal role of a heterologous protein in secreting out of Escherichia coli, Biotechnol Bioeng. 2016 Dec;113(12):2561-2567. doi: 10.1002/bit.26028. Epub 2016 Jun 14.
Construction of cellulose-utilizing Escherichia coli based on a secretable cellulose, Microb Cell Fact. 2015 Oct 9;14:159. doi: 10.1186/s12934-015-0349-7
C3: Zero-shot Text-to-SQL with ChatGPT
This paper proposes a ChatGPT-based zero-shot Text-to-SQL method, dubbed C3,
which achieves 82.3\% in terms of execution accuracy on the holdout test set of
Spider and becomes the state-of-the-art zero-shot Text-to-SQL method on the
Spider Challenge. C3 consists of three key components: Clear Prompting (CP),
Calibration with Hints (CH), and Consistent Output (CO), which are
corresponding to the model input, model bias and model output respectively. It
provides a systematic treatment for zero-shot Text-to-SQL. Extensive
experiments have been conducted to verify the effectiveness and efficiency of
our proposed method
Phase-locking matter-wave interferometer of vortex states
Matter-wave interferometer of ultracold atoms with different linear momenta
has been extensively studied in theory and experiment. The vortex matter-wave
interferometer with different angular momenta is applicable as a quantum sensor
for measuring the rotation, interatomic interaction, geometric phase, etc. Here
we report the first experimental realization of a vortex matter-wave
interferometer by coherently transferring the optical angular momentum to an
ultracold Bose condensate. After producing a lossless interferometer with atoms
only populating the two spin states, we demonstrate that the phase difference
between the interferences in the two spin states is locked on . We also
demonstrate the robustness of this out-of-phase relation, which is independent
of the angular-momentum difference between the two interfering vortex states,
constituent of Raman optical fields and expansion of the condensate. The
experimental results agree well with the calculation from the unitary evolution
of wave packet in quantum mechanics. This work opens a new way to build a
quantum sensor and measure the atomic correlation in quantum gases.Comment: 5 figure
Expansion dynamics of a spherical Bose-Einstein condensate
We experimentally and theoretically observe the expansion behaviors of a
spherical Bose-Einstein condensate. A rubidium condensate is produced in an
isotropic optical dipole trap with an asphericity of 0.037. We measure the
variation of the condensate size during the expansion process. The free
expansion of the condensate is isotropic, which is different from that of the
condensate usually produced in the anisotropic trap. The expansion in the short
time is speeding and then after a long time the expansion velocity
asymptotically approaches a constant value. We derive an analytic solution of
the expansion behavior based on the spherical symmetry, allowing a quantitative
comparison with the experimental measurement. The interaction energy of the
condensate is gradually converted into the kinetic energy at the beginning of
the expansion and the kinetic energy dominates after a long-time expansion. We
obtain the interaction energy of the condensate in the trap by probing the
expansion velocity, which is consistent with the theoretical prediction.Comment: 6 pages, 5 figure
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