Management of serology negative human hepatic hydatidosis (caused by Echinococcus granulosus) in a young woman from Bangladesh in a resource-rich setting: A case report

Human cystic echinococcosis (hydatidosis) is a parasitic zoonosis with almost complete worldwide distribution. Echinococcus granulosus, the dog tapeworm, causes hydatidosis which accounts for 95% of human echinococcosis. Although this tapeworm is found in dogs as a definitive host and a number of intermediate hosts, humans are often infected from close contact with infected dogs. Humans are not part of the parasitic lifecycle and serve as accidental hosts. Hydatidosis is an important consideration in the differential diagnosis of hepatic cysts in individuals from endemic areas. Clinicians should be aware of the long incubation period, the high frequency of negative serological tests, and the possibility of intraoperative evaluations of the cyst aspirate being non-diagnostic. We describe a case of serology negative hydatidosis that came to medical attention as an incidental finding in a young woman from Bangladesh. The patient underwent imaging and was then started on albendazole. After several weeks of albendazole, the cyst was punctured, aspirated, injected with hypertonic saline, re-aspirated, and then fully excised. Diagnosis was confirmed by microscopic evaluation of the cyst aspirate. Serological tests for hydatidosis may be negative in patients with early disease and thus should not be used to rule out this disease. Consideration of this diagnosis allows clinicians to avoid the catastrophic spillage of cystic contents risking an anaphylactic reaction, which might prove fatal. Despite World Health Organization hydatidosis staging being based on ultrasound, radiologists in resource-rich setting may prefer MRI in the management and staging of cystic echinococcosis

Effect of Defoliation Management on Expression of the ‘High Sugar’ Cultivar Trait in Tasmania

The proposed benefits of perennial ryegrass (Lolium perenne L.) cultivars with a high concentration of water soluble carbohydrates (WSC) for the dairy industry in Australia include enhanced rumen protein metabolism, and potential improvements in milk components (Stewart et al. 2009). Perennial ryegrass cultivars have been developed to express higher levels of WSC, and extensive trials have shown consistent trait expression in Europe (reviewed by Edwards et al. 2007a). In New Zealand and Australia, there has been less consistent expression of the high sugar trait (Smith et al. 1998; Parsons et al. 2004; Francis et al. 2006), possibly reflecting a genotype by environmental interaction (Parsons et al. 2007). The current study was developed to investigate whether the high sugar trait of cultivars Aber-Magic (developed in Aberystwyth) and SF Joule AR1 (developed in Australia), is consistently expressed in cool temperate Tasmania, Australia, and to quantify the effect of contrasting defoliation management on trait expression. It is also important to confirm that any elevation in WSC concentration does not occur at the expense of dry matter (DM) yield, and to consider the relationship between WSC and crude protein (CP) concentrations - thought to be an important contributor to more efficient nitrogen (N) partitioning (Edwards et al. 2007b)

Preliminary results of parameterisation of DairyMod pasture model for tropical pasture; Brachiaria

Exploring improved tropical forages is considered to be an important approach in delivering quality and consistent feed options in tropical and even subtropical regions under changing climate scenarios. Pasture modelling has been an effective tool in simulating pasture growth and obviating expensive field research under a range of soil, climate, and management strategies. Many models lack parameters for tropical pasture species, hindering their use in tropical regions. This study aims to adapt the generic parameters in the DairyMod pasture model to parameterise and evaluate the model for the tropical pasture species Brachiaria ruziziensis x B. decumbens x B. brizantha ‘Brachiaria Mulato II’ (BM). Data were collected from plots of BM established at the Gatton Research Dairy (27° 54´S, 152°33`E), Queensland, Australia from 19 November 2020 to 06 May 2021 to parameterise the model. Model evaluation was performed through various statistical indices for accuracy and precision. Canopy structure and carbon partitioning, photosynthesis and respiration, senescence and leaf appearance parameters were mainly modified from the generic C4 grass parameters in the model. Results showed that, species specific parameters setup for BM in the model simulated the total above ground yield (R2=0.92), leaf (R2=0.97), and LAI (R2=0.93) at a reasonable accuracy. Stem production also ranged under acceptable level except for the second defoliation due to decreased cutting height. Despite these reasonable simulated results, the model tended to underestimate stem production. Reasons could be higher variation of residual weight across the seasons and model failure to explicitly capture the plant physiological changes like anthesis, accelerated growth rate and increased stem production associated with tropical pasture phenological developments. Results suggest that the developed BM parameters in DairyMod need further testing under range of locations and seasons to improve the model

Effect of Nitrogen Fertiliser Applications on Botanical Composition

The diminishing returns associated with nitrogen (N) fertiliser use over time are well recognised, as are the detrimental effect to the environment of excess N fertiliser use (Eckard 1998). However, many Tasmanian dairy farmers apply continually high rates of N fertiliser (e.g. in excess of 500 kg/ha annually) to minimise risk associated with variable pasture dry matter (DM) yields, and this often occurs at the expense of white clover populations within the pasture (Frame 1990; Frame et al. 1998). The responses of botanical composition and perennial ryegrass DM yields to N fertiliser application rate were investigated in the current field plot study, to provide further information for farmers about the production-related effects of their N use

Plantain (\u3cem\u3ePlantago lanceolata\u3c/em\u3e L.) Growth Islimited Under Waterlogging

Plantain (Plantago lanceolata L.; PL) is becoming an increasingly important component of New Zealand dairy pastures because of its ability to reduce nitrogen losses to the environment. However, widespread suggestions of its poor persistence, have led to industry concern over its suitability for dairy systems. Anecdotal evidence suggests that PL does not cope well in waterlogged soil, yet there is little scientific literature relevant to this issue. Thus, the aim of this study was to investigate the impact of waterlogging stress on PL growth and survival. In a glasshouse, the performance of PL under waterlogging was evaluated against perennial ryegrass (Lolium perenne L.; PRG), the predominant plant species in New Zealand dairy pastures. Three watering regimes were applied to PL and PRG plants in plastic pots: control (optimal watering), wet (soil at field capacity), and waterlogged (water table 5cm below the soil surface) for 39 days, before 27 days of recovery under control watering. Plant dry mass (DM) harvests were made on days 22, 39 and 66. The leaf DM of PL was reduced by the waterlogging treatment by 37% and 38% respectively during the treatment and recovery periods, in comparison with the control. In contrast, the leaf DM of PRG plants was only reduced by 18% and 3% by the waterlogging treatment during the treatment and recovery periods respectively, and so PRG produced 28% and 45% more leaf DM under waterlogging than PL, during the treatment and recovery periods, respectively. The root DM of PRG was 33% higher under waterlogging in comparison with the control at day 39, while PL root DM was not significantly affected by water treatment. Whilst PL growth appears to be sensitive to waterlogging stress, the survival of PL under waterlogged conditions suggests that it possesses some waterlogging tolerance strategies. PRG exhibited an enhanced ability for coping under, and recovering from waterlogging stress, which may have been associated with the maintenance of root growth under waterlogging. These findings suggest that PL growth may be somewhat limited in periodically waterlogged pastures, and the enhanced ability of PRG to cope with waterlogging stress, could provide it with a competitive advantage over PL under such conditions, should they coexist in a mixed sward

Suitability Evaluation of Three Tropical Pasture Species (Mulato II, Gatton Panic, and Rhodes Grass) for Cultivation under a Subtropical Climate of Australia

Exploring improved tropical forages is considered to be an important approach in delivering quality and consistent feed options for dairy cattle in tropical and subtropical regions. The present study aimed to study the suitability of three improved tropical grasses, Chloris gayana ‘Rhodes grass cv. Reclaimer’ (RR), Megathyrsus maximus ‘Gatton Panic’ (GP), and Brachiaria ruziziensis x B. decumbens x B. brizantha ‘Brachiaria Mulato II’ (BM) evaluating their carbon assimilation, canopy structure, herbage plant–part accumulation and quality parameters under irrigated conditions. An experiment was conducted at Gatton Research Dairy (27°54′ S, 152°33′ E, 89 m asl) Queensland, Australia, which has a predominantly subtropical climate. Photosynthesis biochemistry, canopy structure, herbage accumulation, plant part composition, and nutritive value were evaluated. Photosynthesis biochemistry differed between pasture species. Efficiency of CO2 assimilation was highest for GP and quantum efficiency was highest for BM. Pasture canopy structure was significantly affected by an interaction between pasture species and harvest. Forage biomass accumulation was highest in GP, while BM produced more leaf and less stem compared to both GP and RR. A greater leafy stratum and lower stemmy stratum depth were observed in the vertical sward structure of BM. Brachiaria Mulato II showed greater carbon partitioning to leaves, leaf: stem ratio, canopy, and leaf bulk density. It also demonstrated greater nutritive value (Total digestible nutrients (TDN), acid detergent fibre (ADF), neutral detergent fibre (NDF), neutral detergent insoluble protein (NDICP), Starch, nonfibre carbohydrates (NFC), metabolisable energy (ME), mineral profile (Mg, P, K, Fe, Zn) and dietary cation–anion difference (DCAD) for leaf, stem, and the whole plant. Greater quantum efficiency, leaf accumulation, and nutritive value of BM observed in the present study suggest BM as an attractive forage option for dairying that warrants further research in pasture-based systems in tropical and subtropical climates

Suitability Evaluation of Three Tropical Pasture Species (Mulato II, Gatton Panic, and Rhodes Grass) for Cultivation under a Subtropical Climate of Australia

Exploring improved tropical forages is considered to be an important approach in delivering quality and consistent feed options for dairy cattle in tropical and subtropical regions. The present study aimed to study the suitability of three improved tropical grasses, Chloris gayana ‘Rhodes grass cv. Reclaimer’ (RR), Megathyrsus maximus ‘Gatton Panic’ (GP), and Brachiaria ruziziensis x B. decumbens x B. brizantha ‘Brachiaria Mulato II’ (BM) evaluating their carbon assimilation, canopy structure, herbage plant–part accumulation and quality parameters under irrigated conditions. An experiment was conducted at Gatton Research Dairy (27° 54 ′S, 152°33 ′E, 89 m asl) Queensland, Australia, which has a predominantly subtropical climate. Photosynthesis biochemistry, canopy structure, herbage accumulation, plant part composition, and nutritive value were evaluated. Photosynthesis biochemistry differed between pasture species. Efficiency of CO2 assimilation was highest for GP and quantum efficiency was highest for BM. Pasture canopy structure was significantly affected by an interaction between pasture species and harvest. Forage biomass accumulation was highest in GP, while BM produced more leaf and less stem compared to both GP and RR. A greater leafy stratum and lower stemmy stratum depth were observed in the vertical sward structure of BM. Brachiaria Mulato II showed greater carbon partitioning to leaves, leaf: stem ratio, canopy, and leaf bulk density. It also demonstrated greater nutritive value (Total digestible nutrients (TDN), acid detergent fibre (ADF), neutral detergent fibre (NDF), neutral detergent insoluble protein (NDICP), Starch, nonfibre carbohydrates (NFC), metabolisable energy (ME), mineral profile (Mg, P, K, Fe, Zn) and dietary cation–anion difference (DCAD) for leaf, stem, and the whole plant. Greater quantum efficiency, leaf accumulation, and nutritive value of BM observed in the present study suggest BM as an attractive forage option for dairying that warrants further research in pasture-based systems in tropical and subtropical climates

Research diagnostic criteria for mild cognitive impairment with Lewy bodies: A systematic review and meta-analysis.

INTRODUCTION Operationalized research criteria for mild cognitive impairment with Lewy bodies (MCI-LB) were published in 2020. The aim of this systematic review and meta-analysis was to review the evidence for the diagnostic clinical features and biomarkers in MCI-LB set out in the criteria. METHODS MEDLINE, PubMed, and Embase were searched on 9/28/22 for relevant articles. Articles were included if they presented original data reporting the rates of diagnostic features in MCI-LB. RESULTS Fifty-seven articles were included. The meta-analysis supported the inclusion of the current clinical features in the diagnostic criteria. Evidence for striatal dopaminergic imaging and meta-iodobenzylguanidine cardiac scintigraphy, though limited, supports their inclusion. Quantitative electroencephalogram (EEG) and fluorodeoxyglucose positron emission tomography (PET) show promise as diagnostic biomarkers. DISCUSSION The available evidence largely supports the current diagnostic criteria for MCI-LB. Further evidence will help refine the diagnostic criteria and understand how best to apply them in clinical practice and research. HIGHLIGHTS A meta-analysis of the diagnostic features of MCI-LB was carried out. The four core clinical features were more common in MCI-LB than MCI-AD/stable MCI. Neuropsychiatric and autonomic features were also more common in MCI-LB. More evidence is needed for the proposed biomarkers. FDG-PET and quantitative EEG show promise as diagnostic biomarkers in MCI-LB

Full potential LAPW calculation of electron momentum density and related properties of Li

Electron momentum density and Compton profiles in Lithium along $$,$$, and $$ directions are calculated using Full-Potential Linear Augmented Plane Wave basis within generalized gradient approximation. The profiles have been corrected for correlations with Lam-Platzman formulation using self-consistent charge density. The first and second derivatives of Compton profiles are studied to investigate the Fermi surface breaks. Decent agreement is observed between recent experimental and our calculated values. Our values for the derivatives are found to be in better agreement with experiments than earlier theoretical results. Two-photon momentum density and one- and two-dimensional angular correlation of positron annihilation radiation are also calculated within the same formalism and including the electron-positron enhancement factor.Comment: 11 pages, 7 figures TO appear in Physical Review

Plants Modify Biological Processes to Ensure Survival following Carbon Depletion: A Lolium perenne Model

BACKGROUND: Plants, due to their immobility, have evolved mechanisms allowing them to adapt to multiple environmental and management conditions. Short-term undesirable conditions (e.g. moisture deficit, cold temperatures) generally reduce photosynthetic carbon supply while increasing soluble carbohydrate accumulation. It is not known, however, what strategies plants may use in the long-term to adapt to situations resulting in net carbon depletion (i.e. reduced photosynthetic carbon supply and carbohydrate accumulation). In addition, many transcriptomic experiments have typically been undertaken under laboratory conditions; therefore, long-term acclimation strategies that plants use in natural environments are not well understood. METHODOLOGY/PRINCIPAL FINDINGS: Perennial ryegrass (Lolium perenne L.) was used as a model plant to define whether plants adapt to repetitive carbon depletion and to further elucidate their long-term acclimation mechanisms. Transcriptome changes in both lamina and stubble tissues of field-grown plants with depleted carbon reserves were characterised using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The RT-qPCR data for select key genes indicated that plants reduced fructan degradation, and increased photosynthesis and fructan synthesis capacities following carbon depletion. This acclimatory response was not sufficient to prevent a reduction (P<0.001) in net biomass accumulation, but ensured that the plant survived. CONCLUSIONS: Adaptations of plants with depleted carbon reserves resulted in reduced post-defoliation carbon mobilization and earlier replenishment of carbon reserves, thereby ensuring survival and continued growth. These findings will help pave the way to improve plant biomass production, for either grazing livestock or biofuel purposes