The identity of Albuca caudata Jacq. (Hyacinthaceae) and a description of a new related species : A. bakeri

The name Albuca caudata Jacq. has been widely misunderstood or even ignored since its description in 1791. After studying herbarium specimens and living populations in South Africa, plants fitting Jacquin´s concept of that species are found to be widely distributed in the Eastern Cape, mainly in the Albany centre of Endemism. Furthermore, some divergent specimens matching Baker´s concept of Albuca caudata are described as a new related species: Albuca bakeri. Data on typification, morphology, ecology, and distribution are reported for both taxa. Affinities and divergences with other close allies are also discussed

Nicipe rosulata (Ornithogaloideae, Hyacinthaceae), a New Species from the Little Karoo in South Africa, with a New Combination in the Genus

A new species of Nicipe from the Little Karoo in South Africa is here described. Nicipe rosulata sp. nov. is characterized by its (3‐)5‐8 short and broad, somewhat leathery leaves disposed in a basal rosette, its narrowly ovate, acute-apiculate capsules, and its long papillate-echinulate seeds. This species is at first sight related to Nicipe britteniae and Ornithogalum lithopsoides based on their short leaves with ciliate to fimbriate margins, but it differs in floral and vegetative characters that clearly support its recognition as a distinct species. Nicipe britteniae differs from N. rosulata by the hard, distichous, ensiform, conduplicate, and densely fimbriate leaves and the rugose seeds. Ornithogalum lithopsoides clearly differs by the more numerous and much thinner leaves, and the rugose seeds, among other characters. Here we provide a detailed morphological description for Nicipe rosulata, including ecological and chorological data, and discuss relationships with its close allies. Finally, the recently described Ornithogalum lithopsoides, also from the Little Karoo, clearly belongs to Nicipe based on the leathery rosulate leaves all arising nearly at the same level, the relatively small flowers, the tepals with a dark longitudinal band mostly visible on the abaxial side, and the small capsules and seeds. This new combination in the latter genus is also presented here.This work was partly supported by the Fundación Ramón Areces (Spain) and Universidad de Alicante (Spain)

Drimia trichophylla (Hyacinthaceae, Urgineoideae), a New Species from the Eastern Cape Province, South Africa

Within the framework of a taxonomic revision of Drimia sensu lato we here describe a new species from the Eastern Cape Province in South Africa. Drimia trichophylla sp. nov. is at first sight related to D. vermiformis, but it can be clearly differentiated by the flower and leaf morphology and distribution. A complete description is presented for this species, and data on morphology, ecology, and distribution are reported. Affinities and divergences with other close allies are also discussed.This work was partly supported by Fundación Ramón Areces (Spain), H2020 Research and Innovation Staff Exchange Programme of the European Commission, project 645636: 'Insect-plant relationships: insights into biodiversity and new applications' (FlyHigh), University of Alicante (Spain), Rhodes University (Dept. of Botany) and the Selmar Schonland Herbarium (GRA) (Eastern Cape, South Africa)

Austronea patersoniae (Asparagaceae, Scilloideae), a new species from the Eastern Cape Province in South Africa

Our fieldwork in the Eastern Cape province of South Africa revealed an undescribed species of Austronea which was named by Schonland as “Urginea Patersoniae Schonl. Ms.”, but never validly published. We here describe Austronea patersoniae to include plants showing bulbs with loose scales; 5–9 narrowly linear erect leaves with papillate margins; elongate peduncle; lowermost bracts with a broad, flat, papery spur and reddish flowers with papillate filaments. We provide a complete morphological description as well as data on ecology and distribution.This work was partly supported by H2020 Research and Innovation Staff Exchange Programme of the European Commission, project 645636: ' Insect-plant relationships: insights into biodiversity and new applications' (FlyHigh), and the grants ACIEI8-Q3 UAUSTI I8-Q2 from the University of Alicante

The rediscovery of the Great Winterberg endemic Lotononis harveyi B.–E.van Wyk after 147 years, and notes on the poorly known Amathole endemic Macowania revoluta Oliv. (southern Great Escarpment, South Africa)

South Africa’s 800 km-long southern Great Escarpment hosts numerous endemic plant species only known from their type specimens or from very few records. This is a legacy of a 100–150 year lag between the pioneer work of 19th century botanists and repeat fieldwork in the 21st century. As a result, population and ecological data are lacking for many local endemic species. Here we report on the rediscovery of Lotononis harveyi B.–E.van Wyk 147 years after its original description, and provide the first detailed ecological notes on the poorly known shrub Macowania revoluta Oliv. Both species are locally endemic to the Great Winterberg–Amatholes (Eastern Cape Province). With only six known individuals, L. harveyi is recommended the conservation status of Critically Endangered, with fire (and potentially grazing) being the main population constraints. Macowania revoluta is locally abundant, and it is surprising that it has been so poorly collected in recent decades. It occupies an important local niche as a keystone montane wetland species, and its narrow distribution range – combined with pressure from woody alien invasive species – suggests that its conservation status should be Rare. The research further highlights the need for continued biodiversity field research along South Africa’s poorly explored Great Escarpment.The research results were part of two post-graduate studies: a PhD (VRC) supported by the National Research Foundation (NRF, grant GUN 2069059), the South African Biosystematics Initiative (SABI, 2006–2009), the National Geographic Society (USA) Committee for Research and Exploration (grant 8521-08), Buk’Indalo Consultancy cc, a Dudley D’Ewes Scholarship from the Cape Tercentenary Foundation; and a M.Sc. (JB) also supported by the NRF (SABI grant 71072 and an NRF Masters bursary). This paper was constructed during a NRF Scarce Skills Post-doctoral Fellowship (VRC, 2014–2016.http://phytokeys.pensoft.netam2016Physiotherap

Molecular phylogenetics of subfamily Urgineoideae (Hyacinthaceae): Toward a coherent generic circumscription informed by molecular, morphological, and distributional data

The taxonomy and systematics of Urgineoideae (Hyacinthaceae) have been controversial in recent decades, with contrasting taxonomic treatments proposed based on preliminary and partial studies that have focused on morphology and/or solely plastid DNA sequence data. Some authors have recognized only two genera, with a very broadly conceived Drimia, while others have accepted several genera that, although better defined morphologically, were doubtfully monophyletic. Here, we present phylogenetic analyses involving four plastid DNA regions (trnL intron, trnL-F spacer, matK, and the trnCGCA-ycf6 intergenic region), a nuclear region (Agt1), and a selection of 40 morphological characters. Our study covers 293 samples and ca. 160 species of Urgineoideae (ca. 80% of its global diversity). Bayesian inference, maximum likelihood, and maximum parsimony analyses were performed to derive the phylogenetic patterns. The combination of data yielded phylogenetic trees with 31 well-defined clades or lineages, most corresponding to previously described genera, although some have required description or revised circumscription. As with other monocot families, a considerable degree of homoplasy was observed in morphological characters, especially in those groups with unspecialized flowers; nonetheless, consistent syndromes of traditional and novel characters are shown to support clade recognition at genus rank. The forthcoming revised classification of Urgineoideae is outlined here.This work was partly supported by H2020 Research and Innovation Staff Exchange Programme of the European Commission, project 645636: ‘Insect-plant relationships: insights into biodiversity and new applications’ (FlyHigh) and the complementary supporting funds UAUSTI17-03, ACIE17-01, UAUSTI2019-008 (University of Alicante, Spain)

Austronea (Asparagaceae, Scilloideae), a new genus from southern Africa, including the description of seven new species

As part of a taxonomic revision of Urgineeae, combining morphological and genetic data from numerous samples from a large range of its distribution, here we describe Austronea, a new genus from South Africa and southern Namibia. This genus is related to Fusifilum based on general inflorescence and flower morphology, but it differs from it by the leaves usually leathery and sometimes thickened; the capitate to subcorymbose raceme commonly nodding at early developing stages; the reddish to green-yellowish tepals, rarely white (see flower buds), which are usually connate at the base to form a distinct cup and patent free lobes, rarely tepals nearly free from the base; the filaments linear to lanceolate, smooth or rarely papillate below; and the ovary green to yellow-orange. These differences are also supported by our genetic studies (not shown) in which Fusifilum and Austronea form two well supported sister clades. Seven new species of Austronea are described from South Africa and Namibia and 11 new combinations in the genus are presented. An identification key is provided for all 18 accepted species in the new genus.This work was partly supported by H2020 Research and Innovation Staff Exchange Programme of the European Commission, project 645636: ‘Insect-plant relationships: insights into biodiversity and new applications’ (FlyHigh) and the complementary supporting funds UAUSTI17-03 and ACIE17-01 (University of Alicante, Spain)

The intestinal expulsion of the roundworm Ascaris suum is associated with eosinophils, intra-epithelial T cells and decreased intestinal transit time

Ascaris lumbricoides remains the most common endoparasite in humans, yet there is still very little information available about the immunological principles of protection, especially those directed against larval stages. Due to the natural host-parasite relationship, pigs infected with A. suum make an excellent model to study the mechanisms of protection against this nematode. In pigs, a self-cure reaction eliminates most larvae from the small intestine between 14 and 21 days post infection. In this study, we investigated the mucosal immune response leading to the expulsion of A. suum and the contribution of the hepato-tracheal migration. Self-cure was independent of previous passage through the liver or lungs, as infection with lung stage larvae did not impair self-cure. When animals were infected with 14-day-old intestinal larvae, the larvae were being driven distally in the small intestine around 7 days post infection but by 18 days post infection they re-inhabited the proximal part of the small intestine, indicating that more developed larvae can counter the expulsion mechanism. Self-cure was consistently associated with eosinophilia and intra-epithelial T cells in the jejunum. Furthermore, we identified increased gut movement as a possible mechanism of self-cure as the small intestinal transit time was markedly decreased at the time of expulsion of the worms. Taken together, these results shed new light on the mechanisms of self-cure that occur during A. suum infections

New combinations in the tribe Urgineeae (Asparagaceae subfam. Scilloideae) with comments on contrasting taxonomic treatments

As part of a taxonomic revision of tribe Urgineeae, and informed by morphological and phylogenetic evidence obtained in the last decade, we present 17 new combinations in Austronea, Indurgia, Schizobasis, Tenicroa, Thuranthos, Urgineopsis, and Vera-duthiea. These are for taxa recently described in Drimia sensu latissimo or otherwise named during the past century. We include type information for all considered taxa and designate lectotypes for Drimia pauciflora, Urginea salmonea and U. sebirii. We discuss recent analytic and synthetic approaches to taxonomic arrangements for the Urgineeae and reinforce the support of an analytic treatment that recognises several genera characterised by distinct syndromes of morphological characters, biogeography and molecular evidence.This work was partly supported by H2020 Research and Innovation Staff Exchange Programme of the European Commission, project 645636: ‘Insect-plant relationships: insights into the biodiversity and new applications’ (FlyHigh), and the grants ACIE18-03 and UAUSTI18-02 from University of Alicante

Evolution of long-term vaccine-induced and hybrid immunity in healthcare workers after different COVID-19 vaccine regimens

BACKGROUND: Both infection and vaccination, alone or in combination, generate antibody and T cell responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the maintenance of such responses-and hence protection from disease-requires careful characterization. In a large prospective study of UK healthcare workers (HCWs) (Protective Immunity from T Cells in Healthcare Workers [PITCH], within the larger SARS-CoV-2 Immunity and Reinfection Evaluation [SIREN] study), we previously observed that prior infection strongly affected subsequent cellular and humoral immunity induced after long and short dosing intervals of BNT162b2 (Pfizer/BioNTech) vaccination. METHODS: Here, we report longer follow-up of 684 HCWs in this cohort over 6-9 months following two doses of BNT162b2 or AZD1222 (Oxford/AstraZeneca) vaccination and up to 6 months following a subsequent mRNA booster vaccination. FINDINGS: We make three observations: first, the dynamics of humoral and cellular responses differ; binding and neutralizing antibodies declined, whereas T and memory B cell responses were maintained after the second vaccine dose. Second, vaccine boosting restored immunoglobulin (Ig) G levels; broadened neutralizing activity against variants of concern, including Omicron BA.1, BA.2, and BA.5; and boosted T cell responses above the 6-month level after dose 2. Third, prior infection maintained its impact driving larger and broader T cell responses compared with never-infected people, a feature maintained until 6 months after the third dose. CONCLUSIONS: Broadly cross-reactive T cell responses are well maintained over time-especially in those with combined vaccine and infection-induced immunity ("hybrid" immunity)-and may contribute to continued protection against severe disease