Unified Modeling of Unconventional Modular and Reconfigurable Manipulation System

Customization of manipulator configurations using modularity and reconfigurability aspects is receiving much attention. Modules presented so far in literature deals with the conventional and standard configurations. This paper presents the 3D printable, light-weight and unconventional modules: MOIRs' Mark-2, to develop any custom `n'-Degrees-of-Freedom (DoF) serial manipulator even with the non-parallel and non-perpendicular jointed configuration. These unconventional designs of modular configurations seek an easy adaptable solution for both modular assembly and software interfaces for automatic modeling and control. A strategy of assembling the modules, automatic and unified modeling of the modular and reconfigurable manipulators with unconventional parameters is proposed in this paper using the proposed 4 modular units. A reconfigurable software architecture is presented for the automatic generation of kinematic and dynamic models and configuration files, through which, a designer can design, validate using visualization, plan and execute the motion of the developed configuration as required. The framework developed is based upon an open source platform called as Robot Operating System (ROS), which acts as a digital twin for the modular configurations. For the experimental demonstration, a 3D printed modular library is developed and an unconventional configuration is assembled, using the proposed modules followed by automatic modeling and control, for a single cell of the vertical farm setup

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Optimal Compositions using Unconventional Modular Library for Customized Manipulators

This paper presents an optimization approach for generating custom manipulator configurations using a proposed unconventional modular library. An end-to-end solution is presented in which the resulting optimal models of the modular compositions can be integrated directly with the Robot Operating System platform. The approach utilizes an unconventional modular library, which is adaptable to a wide range of parameters for customization including non-parallel and non-perpendicular joint axes, and the unified modeling technique for getting the custom modular configurations. The single objective function optimization problem is formulated based upon the discrete parameters of reconfiguration depending upon the available modular library such as, number of joint modules, skew-twist angle, intersecting-twist angle, connection ports of the module, module size, modular sub-assembly unit and curved links. Two case studies, including an application to the agricultural vertical farms, are presented to validate the results