Medicinal plants sub-sector review - Pharmacopoeia promoting programme: Preparatory Study

Prospect Consult Ed. Bruxelles (93 pages + 218 pages annexes)20/SC/96info:eu-repo/semantics/publishe

A strategy for the mapping of N-glycans by high-performance capillary electrophoresis

We have evaluated high-performance capillary electrophoresis (HPCE) with respect to its suitability for use in establishing a carbohydrate-mapping database that would enable a carbohydrate structural analysis by mere comparison of migration times. The suitability of HPCE for carbohydrate structural assignments was ascertained by validation experiments. The migration times of distinct N-glycans, prepared and measured on different days, were shown to be highly reproducible, with a coefficient of variation of usually less than 0.20%, requiring only femtomoles of N-glycan per injection for reliable measurements. By including mesityl oxide and sialic acid as internal standards and a triple-correction method, HPCE fulfills the analytical requirements with respect to accuracy, precision, reproducibility, and sensitivity. The N-glycan-mapping database was established using a newly developed and optimized buffer system containing 1,5-diaminopentane as an organic modifier. Approximately 80 different sialylated N-glycans of known structure, which have thus far been measured and characterized, have been entered into our Lotus 1-2-3 mapping database. The database for structural determinations was tested using the N-linked carbohydrates released from recombinant human urinary erythropoietin (baby hamster kidney) by PNGase F treatment and from bovine serum fetuin and alpha1-acid glycoprotein by automated and manual (large-scale) hydrazinolysis, respectively. The efficiency of the database and of the triple-correction method was further confirmed by HPCE measurements performed in a different laboratory and by a different analyst who used the HPCE system of a different manufacturer

Piani di assistenza infermieristica. Linee guida per un'assistenza personalizzata.

questo libro è pensato per gli studenti che troveranno utili i piani di assistenza per apprendere e sviluppare capacità nella pianificazione del "processo di nursing" e nell'utilizzo delle diagnosi infermieristiche; completerà il loro studio e supporterà l'uso del pensiero critico: Inoltre, la monografia fornisce anche un immediato riferimento per gli infermieri clinici e funge da catalizzatore per la riflessione sulla pianificazione , valutazione e documentazione dell'assistenza infermieristica