Mitigatory Effects of Quercetin on Bisphenol A-Induced Oxidative Stress in Testis of Swiss Albino Mice

Background: Bisphenol A is a well-known xenoestrogen, mainly used in plastics and food can liners manufacture. Hence, it becomes an integral part of the food chain. Experimental studies shows negative health effects. Quercetin is one of the most effective antioxidants of the flavonoids, generally present in food stuff. The present study was an attempt to assess the mitigatory effects of quer on BPA–induced reduction in fertility of mice.Materials and methods: Inbred male Swiss strain albino mice were orally administered with various doses of BPA for 45 days. In another experiment quer along with high dose of BPA was administered to study the mitigatory consequence.Result: Administration of BPA caused significant (P0.05) and dose-dependent increase in oxidative stress compared to control groups. Administration of quer along with high dose of BPA caused amelioration in enzymatic and non-enzymatic antioxidant. Histopathological alteration was also seen in BPA treated groups, which were ameliorated on administration of quer.Conclusion: Quer is potent enough to mitigate the toxic effect of BPA. Biochemical alteration was mitigated by quer and dose dependent increase in fertility was observed

In vivo analysis of Bisphenol A induced dose-dependent adverse effects in cauda epididymis of mice

Bisphenol A is widely used as a material for the production of epoxy resins and polycarbonate plastics. It contaminates various food stuffs by getting leached out from their container lining. Limited information is available on its effects on the male reproductive system. The aim of the present study was to evaluate the extent to which bisphenol A can affect the reproductive system by measuring biochemical and histological changes in the epididymis. Inbred Swiss strain male albino mice were orally administered 80, 120 and 240 mg/kg body weight/day of BPA for 45 days. After completion of treatment, the animals were sacrificed; cauda epididymis was isolated, weighed, used for biochemical and histopathological studies. The results revealed that BPA administered for 45 days caused significant (p<0.05) and dose-dependent reduction in epididymis weight. There was significant (p<0.05) increase in lipid peroxidation and the acid phosphatase activity. Dose dependent reduction in protein, sialic acid contents, as well as the activity of enzymatic antioxidants and mitochondrial enzymes was recorded compared to vehicle treated group. The effect was dose-dependent. Histopathological alteration was observed. This study concludes that BPA causes toxicity in epididymis of mice by generating free radicals, which may be a possible reason for reduction in sperm parameters

Diethanolamine-Induced Hepatic Steatosis in Mice and Its Amelioration by Curcumin

Extensive use of chemicals in personal care products has led to many health issues. Diethanolamine is one of such harmful chemicals containing two highly functional groups alcohol and amine that requires toxicological evaluation and its mitigation. Swiss strain albino mice were used and divided into different control and treated groups. Different doses of DEA (110, 165 and 330 mg/kg body weight/day) were orally administered for 30 days. Biochemical and histopathological assessments were performed at the end of the treatment. Results collectively revealed body weight loss as well as significant increase in absolute and relative liver weight in DEA-treated groups. Biochemical analysis revealed that DEA treatment further promotes significant (P<0.05), dose-dependent increase in lipid and cholesterol contents and also cause decrease in protein and glycogen content. Histopathological assessment confirms vacuole formation due to accumulation of lipid within the liver tissue. Administration of curcumin (10, 20 and 30 mg/kg body weight/day) along with high dose of DEA (330 mg/kg body weight/day) showed improved values of lipid, cholesterol, protein and glycogen contents. It also helped retaining normal histological structure of liver. Observations in all groups and results indicate DEA-treatment causes hepatic steatosis and treatment of curcumin attenuated effect of DEA that is due to its potential antioxidant properties