School Courses as a Motivational Factor for the Implementation of Active Recreation in Adulthood

Abstract The motivation of sports occupies an even more important role than actually performing physical activities. The Ministry of Education of the Slovak Republic ensures the implementation of several compulsory elective sports courses through different stages of educational institutions. We were curious how these courses -especially school in nature -act upon vacations in adulthood. By examining curricular documents and implementing questionnaire methods -applying ,,Vacation practice questionnaire" -we questioned the Hungarian language speakers of southern Slovakia n = 2965. The data were processed using the statistic program SPSS 17. 84.5 % (p < 0.01), of the canvassed took part in school in nature, from which 65,8 % (p < 0.01), rated the time spent there as positive. Amongst the positively valuing 44,2 % indicated the mountains as a favourite and frequently chosen travel destination. 25 % spends more than four hours hiking. We may say that organizing schools in nature has to be considered as an important event (5-7 days), where the students have fun, wherefore they will choose mountains as their travel destination more frequently, which results in higher physical activity. Listed partial discoveries are included in the grant: VEGA no. 1/0376/14 Physical activity intervention for the prevention of health of the population of Slovakia

School Courses as a Motivational Factor for the Implementation of Active Recreation in Adulthood

The motivation of sports occupies an even more important role than actually performing physical activities. The Ministry of Education of the Slovak Republic ensures the implementation of several compulsory elective sports courses through different stages of educational institutions. We were curious how these courses - especially school in nature - act upon vacations in adulthood. By examining curricular documents and implementing questionnaire methods – applying ,,Vacation practice questionnaire” - we questioned the Hungarian language speakers of southern Slovakia n = 2965. The data were processed using the statistic program SPSS 17. 84.5 % (p < 0.01), of the canvassed took part in school in nature, from which 65,8 % (p < 0.01), rated the time spent there as positive. Amongst the positively valuing 44,2 % indicated the mountains as a favourite and frequently chosen travel destination. 25 % spends more than four hours hiking. We may say that organizing schools in nature has to be considered as an important event (5-7 days), where the students have fun, wherefore they will choose mountains as their travel destination more frequently, which results in higher physical activity. Listed partial discoveries are included in the grant: VEGA no. 1/0376/14 Physical activity intervention for the prevention of health of the population of Slovakia

A szabadidős sport keresleti és kínálati elemek vizsgálata az Észak-alföldi régió egészségturizmusában

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An improved and widely accessible dNTP quantitation tool

Cells maintain a fine-tuned concentration balance in the pool of deoxyribonucleoside 5’- triphosphates (dNTPs). The perturbation of this balance results in increased mutation frequencies suggested to promote cancer development and drug resistance. To study dNTP imbalances and their consequences, an accurate and relatively high-throughput method is necessary. The dNTP quantitation method of our choice is a fluorescence-based, TaqMan-like polymerase assay published by Wilson et al, NAR 2011. This assay has the advantages of being accessible in a standard molecular biology laboratory and having the potential to be automated in contrast to mass spectrometry or radioactive measurements. Although this method works well in diluted samples with high dNTP levels, we observed that the sample matrix largely decreases assay performance. Upon thorough kinetic analysis of the fluorescent dNTP incorporation curves, we found that the Taq polymerase exhibits a dNTP independent, signal generating exonuclease activity and that the polymerization and exonuclease activity are partially inhibited by the sample matrix. Based on our kinetic investigations we suggest several assay modifications and a novel, kineticsbased and automated analysis method. Using these modifications, we measured dNTP pools in widely different organisms including Mycobacterium smegmatis, Staphylococcus aureus and human cancer cells. We found that our improved method is capable of i) determining dNTP concentrations in samples previously proved to be unmeasurable by eliminating the interfering matrix effect, and ii) improving the quantitation limits of the assay. Fundings: NKFIH-PD 124330, NKFIH-K 115993, János Bolyai Research Scholarshi

Health of Adults Through Prism of Physical Activity

The aim of the research was to find out the realization of the physical activity among the middle-aged adults by the association with their health, retrospective of the factor contributing to the transfer of the physical activity to the adulthood. The monitored group consisted of 742 respondents of the middle-aged adults from the Southern Districts of Slovakia, of which 403 were the women (age = 37.2 ± 3.04 years) and 339 were the men (age = 36.5 ± 4.54 years) as the selection was deliberate. The monitoring was conducted by the three stages in 2014, through the so-called “Egészség és mozgás” - “Health and Movement” standardized, anonymous questionnaire, which consisted of 60 questions. Our findings point to the stated facts. The findings found the relationship between the health and fitness among the men (r = 0.8300), as well as among the women (r = 0.7193). The relationship between the physical activity and the feeling of health was recorded only among the men (r = 0.8921), while the relationship between the health problems and the feeling of health was also found among the men (r = 0.739), as well as among the women (r = 0, 6714). At the same time, the men perceive the importance of the physical activity, in terms of their health condition (r = 0.8791) more intense than the women. The physical education was significantly (χ2 = 112.47, p < 0.01) among the men (67.6 %, n = 229), opposite to the women (33.7 %; n = 136) contributed to the transfer of the physical activity from the school environment to the adulthood. The stated findings show that the targeted education of the population, even from the childhood with the other effective, preventive measures is very important