Accuracy Assessment of Classical Isothermal Experiment in Drug Storage Period Studies

The purpose of this research was to assessment the effect of change of dissolved oxygen concentration on accuracy of classical isothermal experiment in ascorbic acid solution storage period studies. The experiments were performed at temperatures 35 ˚C, 40 ˚C, 45 ˚C and 50 ˚C, the apparent rate constant (kA) and the adjusted apparent rate constant (kA,A) were determined, respectively. By plotting lnkA and lnkA,A against 1/T resulted two lines, respectively. Then the apparent rate constant at 25 ˚C, kA,25˚C =5.168×10-4(mol×L-1×h-1) and the adjusted apparent rate constant at 25 ˚C, kA,A,25˚C=5.157×10-4(mol×L-1×h-1), was extrapolated, respectively. Both the calculated storage period of the experimented ascorbic acid solution were all 55h by kA,25˚C and kA,A,25˚C， respectively. The results suggested that the change of dissolved oxygen concentration has no effect on the accuracy of classical isothermal experiment in ascorbic acid solution storage period studies

Accuracy Assessment of Classical Isothermal Experiment in Drug Storage Period Studies

The purpose of this research was to assessment the effect of change of dissolved oxygen concentration on accuracy of classical isothermal experiment in ascorbic acid solution storage period studies. The experiments were performed at temperatures 35 ˚C, 40 ˚C, 45 ˚C and 50 ˚C, the apparent rate constant (kA) and the adjusted apparent rate constant (kA,A) were determined, respectively. By plotting lnkA and lnkA,A against 1/T resulted two lines, respectively. Then the apparent rate constant at 25 ˚C, kA,25˚C =5.168×10-4(mol×L-1×h-1) and the adjusted apparent rate constant at 25 ˚C, kA,A,25˚C=5.157×10-4(mol×L-1×h-1), was extrapolated, respectively. Both the calculated storage period of the experimented ascorbic acid solution were all 55h by kA,25˚C and kA,A,25˚C， respectively. The results suggested that the change of dissolved oxygen concentration has no effect on the accuracy of classical isothermal experiment in ascorbic acid solution storage period studies

The Impact of COVID-19 on the Mental Health and Wellbeing of Children with Special Education Needs and Disabilities: A Systematic Review

The COVID-19 pandemic has impacted greatly the mental health of children with special educational needs and disabilities (SEND). We performed a systematic review to investigate whether the impact of the pandemic was universal or restricted to specific groups. Following PRISMA guidelines, of 2500 search results, 66 studies were included in our analysis as they met our inclusion criteria concerning: a) children with SEND; b) focus on COVID-19; c) longitudinal or cross-sectional design; d) quantitative or qualitative measures; and e) mental health or wellbeing outcomes. Our review suggests that there was a negative change in mental health and wellbeing universally for children with SEND

Frequency detection of BRAF V600E mutation in a cohort of pediatric langerhans cell histiocytosis patients by next-generation sequencing

Abstract Background Langerhans cell histiocytosis (LCH) is a rare neoplastic disease that occurs in both children and adults, and BRAF V600E is detected in up to 64% of the patients. Several studies have discussed the associations between BRAF V600E mutation and clinicopathological manifestations, but no clear conclusions have been drawn regarding the clinical significance of the mutation in pediatric patients. Results We retrieved the clinical information for 148 pediatric LCH patients and investigated the BRAF V600E mutation using next-generation sequencing alone or with droplet digital PCR. The overall positive rate of BRAF V600E was 60/148 (41%). The type of sample (peripheral blood and formalin-fixed paraffin-embedded tissue) used for testing was significantly associated with the BRAF V600E mutation status (p-value = 0.000 and 0.000). The risk of recurrence declined in patients who received targeted therapy (p-value = 0.006; hazard ratio 0.164, 95%CI: 0.046 to 0.583). However, no correlation was found between the BRAF V600E status and gender, age, stage, specific organ affected, TP53 mutation status, masses close to the lesion or recurrence. Conclusions This is the largest pediatric LCH study conducted with a Chinese population to date. BRAF V600E in LCH may occur less in East Asian populations than in other ethnic groups, regardless of age. Biopsy tissue is a more sensitive sample for BRAF mutation screening because not all of circulating DNA is tumoral. Approaches with low limit of detection or high sensitivity are recommended for mutation screening to avoid type I and II errors

B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection

A new antibody diagnostic assay with more rapid and robust properties is demanded to quantitatively evaluate anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunity in a large population. Here, we developed a nanometer-scale fluorescent biosensor system consisting of CdSe-ZnS quantum dots (QDs) coupled with the highly sensitive B-cell epitopes of SARS-CoV-2 that could remarkably identify the corresponding antibody with a detection limit of 100 pM. Intriguingly, we found that fluorescence quenching of QDs was stimulated more obviously when coupled with peptides than the corresponding proteins, indicating that the energy transfer between QDs and peptides was more effective. Compared to the traditional enzyme-linked immunosorbent assay (ELISA), the B-cell-epitope-based QD-biosensor could robustly distinguish coronavirus disease 2019 (COVID-19) antibody-positive patients from uninfected individuals with a higher sensitivity (92.3–98.1% positive rates by QD-biosensor vs. 78.3–83.1% positive rates by ELISAs in 207 COVID-19 patients’ sera) in a more rapid (5 min) and labor-saving manner. Taken together, the ‘QD-peptides’ biosensor provided a novel real-time, quantitative, and high-throughput method for clinical diagnosis and home-use tests