Eicosapentaenoic acid influences the pathogenesis of Candida albicans in Caenorhabditis elegans via inhibition of hyphal formation and stimulation of the host immune response

DATA AVAILABILITY : All data generated during this study are included in this article and supplementary data.The intake of omega-3 polyunsaturated fatty acids, including eicosapentaenoic acid (EPA), is associated with health benefits due to its anti-inflammatory properties. This fatty acid also exhibits antifungal properties in vitro. In order to determine if this antifungal property is valid in vivo, we examined how EPA affects Candida albicans pathogenesis in the Caenorhabditis elegans infection model, an alternative to mammalian host models. The nematodes were supplemented with EPA prior to infection, and the influence of EPA on C. elegans lipid metabolism, survival and immune response was studied. In addition, the influence of EPA on hyphal formation in C. albicans was investigated. It was discovered that EPA supplementation changed the lipid composition, but not the unsaturation index of C. elegans by regulating genes involved in fatty acid and eicosanoid production. EPA supplementation also delayed killing of C. elegans by C. albicans due to the inhibition of hyphal formation in vivo, via the action of the eicosanoid metabolite of EPA, 17,18-epoxyeicosatetraenoic acid. Moreover, EPA supplementation also caused differential expression of biofilm-related gene expression in C. albicans and stimulated the immune response of C. elegans. This provides a link between EPA and host susceptibility to microbial infection in this model.The National Research Foundation (NRF) of South Africa. Open access funding provided by University of the Free State.https://link.springer.com/journal/430hj2024BiochemistryGeneticsMicrobiology and Plant PathologyNon

Measurement of eddy current conductivity

SIGLELD:6029.315(NPL-DES--77) / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Measuring Water Content Heterogeneity Using Multifold GPR with Reflection Tomography

Continuous multioffset acquisition of ground penetrating radar (GPR) data provides the capability to measure the lateral and vertical distribution of soil moisture. Multioffset data enable measurement of radar velocity, which in turn allows the estimation of soil moisture through an appropriate petrophysical relationship. Although rarely used in GPR investigations, reflection tomography coupled with prestack depth migration has the ability to measure lateral velocity variations with much greater resolution and accuracy than conventional methods of velocity analysis. I used reflection tomography in the post-migration domain to estimate radar velocity and the Topp equation to estimate subsurface moisture distribution in two and three dimensions. At a contaminated site near a former refinery I identified a near-vertical boundary separating coarse-grained sands and gravels from a unit containing a high fraction of silts and clays. At a chlorinated solvent waste site, I found significant heterogeneity in the moisture content distribution despite apparent homogeneity indicated by direct push methods