288 research outputs found
Evaluation of the Progeny of Beef Sires Differing in Genetic Merit
End of project reportThe Irish Cattle Breeding Federation (ICBF) publishes breeding values (BVs) for beef bulls. Historically, BVs were expressed in index form relative to the base population. Sometime ago this changed to expression in units of measurement of trait. This change occurred in the course of this project and was accompanied by some re-ranking of bulls. BVs are published for growth, carcass grades and calving traits. Growth BV is
expressed as carcass weight but there is no indication if this results from higher
live weight gain or from a higher kill-out proportion and there is no indication of any
consequences for feed intake or efficiency.
• The objectives of the project were (i) to compare progeny of bulls of high and low
growth genetic index, for growth, feed intake, slaughter traits and carcass traits, (ii)
to partition the extra live weight of progeny of high growth index bulls into carcass
and non-carcass parts, and (iii) to partition any extra carcass weight of progeny
from high growth index bulls into its component fat, muscle and bone fractionsEuropean Unio
Performance and carcass traits of progeny of Limousin sires differing in genetic merit
peer-reviewedGenetic indices for growth and carcass classification are published for beef sires used in Ireland for artificial insemination (AI). The objective of this study was to compare growth and carcass traits of progeny of Limousin sires of low and high genetic index for growth. A total of 70 progeny (42 males and 28 females) out of predominantly Holstein-Friesian cows by 7 AI Limousin sires were reared together to slaughter. The 7 sires were classified as low (n=3) or high (n=4) index based on their published genetic index for growth. The male progeny were reared entire and all animals were slaughtered at about 20 months of age. Carcasses were classified for conformation and fatness, and a rib joint (ribs 6 to 10) was separated into fat, muscle and bone. Growth rate did not differ significantly between the index groups but tended to be higher for the high index progeny. This higher growth rate, combined with a significantly higher kill out proportion, resulted in carcass weight andcarcass weight per day of age being significantly higher for the high index progeny. Carcass conformation and fat class were not affected by genetic index, nor was the composition of the rib joint. Compared with males, females had a significantly lower growth rate and kill out proportion and, consequently, had a significantly lower carcass weight. The proportions of fat and bone in the rib joint were significantly higher, and the proportion of muscle was significantly lower for females than for males. It is concluded that carcass weight reflected sire group genetic index for growth but feed intake, carcass classification and rib joint composition were not affected
Studies relating to Polyunsaturated Fatty Acid (PUFA) Supplementation and Fertility in Cattle
End of project reportReproductive inefficiency has a significant impact on the economic performance of both dairy and beef herds, particularly in seasonal calving systems. Nutrition plays a fundamental role in reproduction. Furthermore, there is emerging evidence that supplemental dietary polyunsaturated fatty acids (PUFA) may increase cow fertility independent of their role as energy substrates. A number of studies have reported enhanced reproductive performance in dairy cows following dietary supplementation with sources of n-3 PUFA. However the possible mechanisms involved have not been identified and there is some inconsistency in the published literature on this topic. The objective of the research reported was to conduct a holistic examination of the effects of dietary long-chain n-3 PUFA supplementation on metabolic and reproductive responses in cattle. Such information is essential for the appropriate formulation of diets to enhance cow reproductive performance and in particular embryo survival
Site Percolation on Pseudo-Random Graphs
We consider vertex percolation on pseudo-random regular graphs. The
previous study by the second author established the existence of phase
transition from small components to a linear (in ) sized
component, at . In the supercritical regime, our main result
recovers the sharp asymptotic of the size of the largest component, and shows
that all other components are typically much smaller. Furthermore, we consider
other typical properties of the largest component such as the number of edges,
existence of a long cycle and expansion. In the subcritical regime, we
strengthen the upper bound on the likely component size
Studies relating to protein expression in the uterus of the cow
End of project reportEmbryo loss is a major cause of reproductive wastage in the cow. The majority of embryo loss occurs in the first 16 days after fertilisation when the embryo is critically dependent on the maternal uterine environment for survival. Despite the central role of uterine fluid in the normal growth and development of the embryo, there is limited information on the protein composition of these fluids. The main objectives of the studies in this thesis were to examine the protein composition of the bovine uterus during the oestrous cycle and to examine the relationship between the concentration of systemic progesterone and uterine protein expression in the cow.
In the first study, the concentration of retinol-binding protein (RBP) in the bovine uterus was found to vary across the cycle and was 5-15-fold higher (P0.05) of the cycle, which was surprising given that previous studies have indicated that uterine RBP gene expression was positively associated with the concentration of systemic progesterone.
In the second study, IGF binding protein 2 (IGFBP-2), IGFBP-3, IGFBP-4 and IGFBP-5 were identified in uterine fluid on days 3, 7, 11 and 15 of the oestrous cycle. There was a local effect on the concentration of IGFBPs where the concentration was greater on the ipsilateral side than that on the contralateral side for IGFBP-2 (P<0.05), 3 (P<0.01) and 5 (P<0.01) on day 15. This difference is a further indication of a local controlling mechanism regulating proteins between the uterine horns. Similar to RBP expression this study could find no significant relationship between the concentration of systemic progesterone and IGFBP concentrations on Day 7 of the oestrous cycle.
In the third study, changes in the global pattern of uterine proteins between Days 3 and Day 15 of the oestrous cycle were examined using two-dimensional electrophoresis (2-DE). Six proteins were found to be upregulated on Day 15 compared to Day 3. Three proteins of these were identified as aldose reductase, plakoglobin and heat shock protein 27 while the other three proteins were identified as bovine serum albumin. Aldose reductase, an enzyme directly involved in the production of sorbitol and indirectly of fructose, was 10-fold higher (P<0.0001) on Day 15 compared to Day 3. Plakoglobin (Pg) was upregulated 2.3-fold (P<0.0001) on Day 15 compared to Day 3. Pg is a component of cellular junctions and its up-regulation may have a role in the uterine glandular epithelium. Heat shock protein 27 (Hsp27) was higher on Day 15 than on Day 3 (P<0.01) and Hsp27 was 1.4-fold higher in the ipsilateral compared to the contralateral uterine horn (P<0.01). Hsp27 may be secreted in response to potential stresses in the uterus or act as a molecular chaperone. On Day 7 there was no difference (P<0.05) in the pattern of proteins secreted between cows with low (2.7±0.10ng/ml) and high (4.8±0.13 ng/ml) concentrations of systemic progesterone on Day 7.
The results of these studies have shown that dramatic changes occur in protein expression across the bovine oestrous cycle. Additionally, it emphasises the need for gene studies to be followed with protein studies as an adjunct or complementary tool. Proteins have a wide range of essential roles in the uterus and together these studies provide novel information on protein expression in the uterus of the cow
Global gene expression in endometrium of high and low fertility heifers during the mid-luteal phase of the estrous cycle
peer-reviewedBackground
In both beef and dairy cattle, the majority of early embryo loss occurs within the first 14 days following insemination. During this time-period, embryos are completely dependent on their maternal uterine environment for development, growth and ultimately survival, therefore an optimum uterine environment is critical to their survival. The objective of this study was to investigate whether differences in endometrial gene expression during the mid-luteal phase of the estrous cycle exist between crossbred beef heifers ranked as either high (HF) or low fertility (LF) (following four rounds of artificial insemination (AI)) using the Affymetrix® 23 K Bovine Gene Chip.
Results
Conception rates for each of the four rounds of AI were within a normal range: 70–73.3%. Microarray analysis of endometrial tissue collected on day 7 of the estrous cycle detected 419 differentially expressed genes (DEG) between HF (n = 6) and LF (n = 6) animals. The main gene pathways affected were, cellular growth and proliferation, angiogenesis, lipid metabolism, cellular and tissue morphology and development, inflammation and metabolic exchange. DEG included, FST, SLC45A2, MMP19, FADS1 and GALNT6.
Conclusions
This study highlights, some of the molecular mechanisms potentially controlling uterine endometrial function during the mid-luteal phase of the estrous cycle, which may contribute to uterine endometrial mediated impaired fertility in cattle. Differentially expressed genes are potential candidate genes for the identification of genetic variation influencing cow fertility, which may be incorporated into future breeding programmes.Teagasc Walsh Fellowship Programm
Biotechnology in Cattle Reproduction.
End of Project ReportTeagasc acknowledges support from the European Union 3rd and 4th Framework Programmes (CT-92-0163 and CT-95-0190)Over the next decade the Irish agri-food industry will have to compete in a rapidly changing world environment arising from increased competitiveness, decreased world market prices and increased consumer demands for higher quality, healthier and safer food. To become competitive in this environment the scale and efficiency of production at both farm and factory level will have to increase significantly and this must be achieved with due regard for the protection of the environment and the welfare of animals. New technologies will be needed to achieve this. Biotechnology will be central to the development of these new technologies.
This project has been concerned with the identification and evaluation of biotechnology developments that have the potential to increase reproductive efficiency in cattle. This includes a range of technologies relating to the in vitro production, manipulation, cryopreservation and transfer of cattle embryos. The potential of other emerging technologies such as embryo and sperm sexing, cloning and biopharming or the production of commercially desirable proteins in cows milk are also addressed in this report.European Unoi
Protein Nutrition and Fertility in Cattle.
End of Project ReportThe objectives of the project were to determine the effects of dietary
crude protein intake, on blood concentrations of ammonia and urea and
on fertility, and on the possible biological mechanisms involved. Heifers
were used in all experiments in order to avoid any confounding effects of
lactation and, or, negative energy balance. The results are summarised as
follows.
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Blood urea and ammonia increased linearly with increases in
dietary urea. Fermentable carbohydrate, in the form of
molassed sugar beet pulp (MSBP), was more effective than
barley in reducing blood urea concentration. Plasma ammonia
concentrations were more variable but MSBP was again more
effective than barley in reducing these.
Embryo survival rate was not affected by either dietary crude
protein (CP) or fermentable energy intake irrespective of
whether animals were fed a silage diet supplemented with feed
grade urea or were grazing pasture with a high crude protein
content. Despite generating systemic urea concentrations of up
to 25 mmol/L and systemic ammonia concentrations of up to
242 μmol/L no significant relationship between these
parameters and embryo survival rate was established.
Consistent with these results on embryo survival, elevated
blood urea or ammonia had little effect on the biochemical
composition of oviduct fluid, the environment of the early
developing embryo.
It can be concluded that elevations in systemic concentrations
of ammonia or urea per se, particularly of the magnitude
observed under normal feeding conditions, are unlikely to
impair embryo survival in cattle as a consequence of
disruptions to the oviductal environment
Isoperimetric Inequalities and Supercritical Percolation on High-dimensional Graphs
It is known that many different types of finite random subgraph models
undergo quantitatively similar phase transitions around their percolation
thresholds, and the proofs of these results rely on isoperimetric properties of
the underlying host graph. Recently, the authors showed that such a phase
transition occurs in a large class of regular high-dimensional product graphs,
generalising a classic result for the hypercube.
In this paper we give new isoperimetric inequalities for such regular
high-dimensional product graphs, which generalise the well-known isoperimetric
inequality of Harper for the hypercube, and are asymptotically sharp for a wide
range of set sizes. We then use these isoperimetric properties to investigate
the structure of the giant component in supercritical percolation on
these product graphs, that is, when , where is the
degree of the product graph and is a small enough constant.
We show that typically has edge-expansion . Furthermore, we show that likely contains a linear-sized
subgraph with vertex-expansion . These
results are best possible up to the logarithmic factor in .
Using these likely expansion properties, we determine, up to small
polylogarithmic factors in , the likely diameter of as well as the
typical mixing time of a lazy random walk on . Furthermore, we show the
likely existence of a path of length .
These results not only generalise, but also improve substantially upon the
known bounds in the case of the hypercube, where in particular the likely
diameter and typical mixing time of were previously only known to be
polynomial in
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