The Naked Mole-Rat: An Unusual Organism with an Unexpected Latent Potential for Increased Intelligence?

Naked mole-rats are eusocial, hairless mammals that are uniquely adapted to their harsh, low-oxygen subsurface habitat. Although their encephalization quotient, a controversial marker of intelligence, is low, they exhibit many features considered tell-tale signs of highly intelligent species on our planet including longevity, plasticity, social cohesion and interaction, rudimentary language, sustainable farming abilities, and maintaining sanitary conditions in their self-built complex housing structures. It is difficult to envision how naked mole-rats would reach even higher levels of intelligence in their natural sensory-challenged habitat, but such an evolutionary path cannot be excluded if they would expand their range onto the earth’s surface.EC/FP7/339231/EU/Habitability of Martian Environments: Exploring the Physiological and Environmental Limits of Life/HOM

Evolution of default genetic control mechanisms

We present a model of the evolution of control systems in a genome under environmental constraints. The model conceptually follows the Jacob and Monod model of gene control. Genes contain control elements which respond to the internal state of the cell as well as the environment to control expression of a coding region. Control and coding regions evolve to maximize a fitness function between expressed coding sequences and the environment. 118 runs of the model run to an average of 1.4 x 10^6 `generations' each with a range of starting parameters probed the conditions under which genomes evolved a `default style' of control. Unexpectedly, the control logic that evolved was not significantly correlated to the complexity of the environment. Genetic logic was strongly correlated with genome complexity and with the fraction of genes active in the cell at any one time. More complex genomes correlated with the evolution of genetic controls in which genes were active (`default on'), and a low fraction of genes being expressed correlated with a genetic logic in which genes were biased to being inactive unless positively activated (`default off' logic). We discuss how this might relate to the evolution of the complex eukaryotic genome, which operates in a `default off' mode.Comment: 25 pages, 8 figures, 1 tabl

Variations in climate habitability parameters and their effect on Earth's biosphere during the Phanerozoic Eon

Essential insights on the characterization and quality of a detectable biosphere are gained by analyzing the effects of its environmental parameters. We compiled environmental and biological properties of the Phanerozoic Eon from various published data sets and conducted a correlation analysis to assess variations in parameters relevant to the habitability of Earth's biosphere. We showed that environmental parameters such as oxygen, global average surface temperatures, runoff rates and carbon dioxide are interrelated and play a key role in the changes of biomass and biodiversity. We showed that there were several periods with a highly thriving biosphere, with one even surpassing present day biodiversity and biomass. Those periods were characterized by increased oxygen levels and global runoff rates, as well as moderate global average surface temperatures, as long as no large or rapid positive and/or negative temperature excursions occurred. High oxygen contents are diagnostic of biomass production by continental plant life. We find that exceptionally high oxygen levels can at least in one instance compensate for decreased relative humidities, providing an even more habitable environment compared to today. Beyond Earth, these results will help us to understand how environmental parameters affect biospheres on extrasolar planets and guide us in our search for extraterrestrial life

Functional Traits Co-Occurring with Mobile Genetic Elements in the Microbiome of the Atacama Desert

Mobile genetic elements (MGEs) play an essential role in bacterial adaptation and evolution. These elements are enriched within bacterial communities from extreme environments. However, very little is known if specific genes co-occur with MGEs in extreme environments and, if so, what their function is. We used shotgun-sequencing to analyse the metagenomes of 12 soil samples and characterized the composition of MGEs and the genes co-occurring with them. The samples ranged from less arid coastal sites to the inland hyperarid core of the Atacama Desert, as well as from sediments below boulders, protected from UV-irradiation. MGEs were enriched at the hyperarid sites compared with sediments from below boulders and less arid sites. MGEs were mostly co-occurring with genes belonging to the Cluster Orthologous Group (COG) categories “replication, recombination and repair,” “transcription” and “signal transduction mechanisms.” In general, genes coding for transcriptional regulators and histidine kinases were the most abundant genes proximal to MGEs. Genes involved in energy production were significantly enriched close to MGEs at the hyperarid sites. For example, dehydrogenases, reductases, hydrolases and chlorite dismutase and other enzymes linked to nitrogen metabolism such as nitrite- and nitro-reductase. Stress response genes, including genes involved in antimicrobial and heavy metal resistance genes, were rarely found near MGEs. The present study suggests that MGEs could play an essential role in the adaptation of the soil microbiome in hyperarid desert soils by the modulation of housekeeping genes such as those involved in energy production.EC/FP7/339231/EU/Habitability of Martian Environments: Exploring the Physiological and Environmental Limits of Life/HOM

The Astrobiology of Alien Worlds: Known and Unknown Forms of Life

Most definitions of life assume that, at a minimum, life is a physical form of matter distinct from its environment at a lower state of entropy than its surroundings, using energy from the environment for internal maintenance and activity, and capable of autonomous reproduction. These assumptions cover all of life as we know it, though more exotic entities can be envisioned, including organic forms with novel biochemistries, dynamic inorganic matter, and self-replicating machines. The probability that any particular form of life will be found on another planetary body depends on the nature and history of that alien world. So the biospheres would likely be very different on a rocky planet with an ice-covered global ocean, a barren planet devoid of surface liquid, a frigid world with abundant liquid hydrocarbons, on a rogue planet independent of a host star, on a tidally locked planet, on super-Earths, or in long-lived clouds in dense atmospheres. While life at least in microbial form is probably pervasive if rare throughout the Universe, and technologically advanced life is likely much rarer, the chance that an alternative form of life, though not intelligent life, could exist and be detected within our Solar System is a distinct possibility.EC/FP7/339231/EU/Habitability of Martian Environments: Exploring the Physiological and Environmental Limits of Life/HOM

Physicochemical Parameters Limiting Growth of Debaryomyces hansenii in Solutions of Hygroscopic Compounds and Their Effects on the Habitability of Martian Brines

The availability of liquid water is a prerequisite for all lifeforms on Earth. In hyperarid subzero environments like the Dry Valleys in Antarctica or the near-subsurface of Mars liquid water might be provided temporarily by hygroscopic substances that absorb water from the atmosphere and lower the freezing point of water. To evaluate the potential of hygroscopic compounds to serve as a habitat, it is necessary to explore the microbial tolerances towards these substances and their life-limiting properties. Here we present a study investigating the tolerances of the halotolerant yeast Debaryomyces hansenii to various solutes. Growth experiments were conducted via counting colony forming units (CFUs) after inoculation of a liquid growth medium containing a specific solute concentration. The lowest water activities (aw) enabling growth were determined to be ~0.83 in glycerol and fructose-rich media. For all other solutes the growth-enabling aw was higher, due to additional stress factors such as chaotropicity and ionic strength. Additionally, we found that the solute tolerances of D. hansenii correlate with both the eutectic freezing point depressions and the deliquescence relative humidities of the respective solutes. Our findings strongly impact our understanding of the habitability of solute-rich low aw environments on Earth and beyond.DFG, 455070607, Salzhaltige Habitate auf dem MarsDFG, 414044773, Open Access Publizieren 2021 - 2022 / Technische Universität Berli

Bacterial Growth in Chloride and Perchlorate Brines: Halotolerances and Salt Stress Responses of Planococcus halocryophilus

Extraterrestrial environments encompass physicochemical conditions and habitats that are unknown on Earth, such as perchlorate-rich brines that can be at least temporarily stable on the martian surface. To better understand the potential for life in these cold briny environments, we determined the maximum salt concentrations suitable for growth (MSCg) of six different chloride and perchlorate salts at 25°C and 4°C for the extremotolerant cold- and salt-adapted bacterial strain Planococcus halocryophilus. Growth was measured through colony-forming unit (CFU) counts, while cellular and colonial phenotypic stress responses were observed through visible light, fluorescence, and scanning electron microscopy. Our data show the following: (1) The tolerance to high salt concentrations can be increased through a stepwise inoculation toward higher concentrations. (2) Ion-specific factors are more relevant for the growth limitation of P. halocryophilus in saline solutions than single physicochemical parameters like ionic strength or water activity. (3) P. halocryophilus shows the highest microbial sodium perchlorate tolerance described so far. However, (4) MSCg values are higher for all chlorides compared to perchlorates. (5) The MSCg for calcium chloride was increased by lowering the temperature from 25°C to 4°C, while sodium- and magnesium-containing salts can be tolerated at 25°C to higher concentrations than at 4°C. (6) Depending on salt type and concentration, P. halocryophilus cells show distinct phenotypic stress responses such as novel types of colony morphology on agar plates and biofilm-like cell clustering, encrustation, and development of intercellular nanofilaments. This study, taken in context with previous work on the survival of extremophiles in Mars-like environments, suggests that high-concentrated perchlorate brines on Mars might not be habitable to any present organism on Earth, but extremophilic microorganisms might be able to evolve thriving in such environments.EC/FP7/339231/EU/Habitability of Martian Environments: Exploring the Physiological and Environmental Limits of Life/HOM

Automated Global Feature Analyzer - A Driver for Tier-Scalable Reconnaissance

For the purposes of space flight, reconnaissance field geologists have trained to become astronauts. However, the initial forays to Mars and other planetary bodies have been done by purely robotic craft. Therefore, training and equipping a robotic craft with the sensory and cognitive capabilities of a field geologist to form a science craft is a necessary prerequisite. Numerous steps are necessary in order for a science craft to be able to map, analyze, and characterize a geologic field site, as well as effectively formulate working hypotheses. We report on the continued development of the integrated software system AGFA: automated global feature analyzerreg, originated by Fink at Caltech and his collaborators in 2001. AGFA is an automatic and feature-driven target characterization system that operates in an imaged operational area, such as a geologic field site on a remote planetary surface. AGFA performs automated target identification and detection through segmentation, providing for feature extraction, classification, and prioritization within mapped or imaged operational areas at different length scales and resolutions, depending on the vantage point (e.g., spaceborne, airborne, or ground). AGFA extracts features such as target size, color, albedo, vesicularity, and angularity. Based on the extracted features, AGFA summarizes the mapped operational area numerically and flags targets of "interest", i.e., targets that exhibit sufficient anomaly within the feature space. AGFA enables automated science analysis aboard robotic spacecraft, and, embedded in tier-scalable reconnaissance mission architectures, is a driver of future intelligent and autonomous robotic planetary exploration