A RAPID METHOD FOR THE FUNCTIONALISATION OF POLYMER SUPPORTS FOR SOLID PHASE OLIGONUCLEOTIDE SYNTHESIS

ABSTRACT A rapid method is described for the covalent anchoring of appropriately protected 2'-deoxyribonucleoside-3'-O-succinates to LCAA-CPG, widely used support for solid phase oligonucleotide synthesis. The method involves the reaction of nucleoside-3'-O-succinates with aminoalkyl functions of the support in the presence of improved and commercially available condensing reagent, TBTU or TPP-DTNP to generate fully functionalised polymer supports with excellent nucleoside loadings