1 research outputs found
Pathomorphologic evaluation of intra-articular injections of soluble platelet‑rich plasma for treatment of experimental osteoarthritis
Introduction Non-surgical treatment of osteoarthritis is aimed at managing joint degeneration
and inflammation to prolong the life of the original joint and delay total joint replacement. The objective
was to pathomorphologically substantiate preclinical effectiveness of PRP in OA using comparative analysis
of depleted plasma and serum.
Material and methods The experiment was performed in 120 Wistar rats, divided into 4 groups. Osteoarthritis
was simulated using an original method. Knee joint injection given to the animals after skin dissection under
inhalation anesthesia and visual control two weeks later contained 0.05 ml PRP in group 1, 0.05 ml plasma
in groups 2 and 0.05 ml blood serum in groups 3. The same volume of physiological saline solution was used
for the injections produced for control animals. Injections were administered three times at 2-week intervals.
Animals were sacrificed in groups of 10 at 2 weeks of each injection.
Results The median MANKIN value scored 2.0 (1.0; 2.0) in group 1, 6.0 (5.0; 7.0) in group 2 and 7.0 (6.0; 7.0)
in group 3 at 6 weeks. The median MANKIN value scored 7.5 (7.0, 8.0) in the control group. Statistically
significant differences were determined between the groups at p < 0.001.
Discussion Literature data on preclinical evaluation of the effectiveness of PRP therapy in biological models
of OA are controversial. An original, low-traumatic functional method was used for simulating knee OA
to reproduce major pathogenetic mechanisms in rats.
Conclusion The findings suggested a pronounced therapeutic effect with improved morphofunctional
features of the hyaline cartilage and MANKIN score of 2 at 6 days of intra-articular administration of modified
PRP as compared with plasma and serum