The Effect of Grown Cell and Cell Free Extract of Rhodococcus sp. and Paenibacillus sp.

Abstract: For first time, biodegradation of Dimethyl Disulfide (DMDS) and Diethyl Disulfide (DEDS) were studied by several bacterial strains, isolated from experimental biofilter filled with these compounds. Among the isolates, two strains identified as Paenibacillus and Rhodococcus could utilize DEDS up to 80.4 % when they were grown on glycerol during 10 days while induced cell free extract of Rhodococcus could remove this compound by 58 % in 30'. Using of DEDS (2 ml l −1) as the sole sulfur source delayed lag phase of Paenibacillus growth for 3 days, when this strain was cultivated on screw capped Castaneda bottles, but growth rate was the same as control. Addition of DMDS (2 ml l −1) reduced the growth rate of both strains; however, Paenibacillus and Rhodococcus could utilize this substrate up to 28.8%. None of these isolates could use DMDS or DEDS as the sole carbon sources. Microplate titer assay showed that using high concentration of DMDS (5 ml l −1) and DEDS (5 ml l −1) did not have positive effect on growth in aerobic condition. However, low concentration of DEDS (5mM) in the same condition increased the growth rate of both strains

Final Author’s Version Single-Ion Solvation Free Energies and the Normal Hydrogen Electrode Potential in

Abstract: The division of thermodynamic solvation free energies of electrolytes into ionic constituents is conventionally accomplished by using the single-ion solvation free energy of one reference ion, conventionally the proton, to set the single-ion scales. Thus, the determination of the free energy of solvation of the proton in various solvents is a fundamental issue of central importance in solution chemistry. In the present article, relative solvation free energies of ions and ion−solvent clusters in methanol, acetonitrile, and dimethyl sulfoxide (DMSO) have been determined using a combination of experimental and theoretical gas-phase free energies of formation, solution-phase reduction potentials and acid dissociation constants, and gas-phase clustering free energies. Applying the cluster pair approximation to differences between these relative solvation free energies leads to values of −263.5, −260.2, and −273.3 kcal/mol for the absolute solvation free energy of the proton in methanol, acetonitrile, and DMSO, respectively. The final absolute proton solvation free energies are used to assign absolute2 values for the normal hydrogen electrode potential and the solvation free energies of other single ions in the solvents mentioned above. 1

2.5. Nitrous Oxide 2.6. Methane

According to the Intergovernmental Panel on Climate Change (IPCC), “the combined radiative forcing due to increases in carbon dioxide, methane, and nitrous oxide is +2.30 [+2.07 to +2.35] W m-2, and its rate o

Synthesis, Molecular Structure and Spectral Properties of Quaternary Ammonium Derivatives of

Abstract: 1,1-Dimethyl-3-oxo-1,4-diazepan-1-ium chloride (1) and 1,1-dimethyl-1carboxymethyl-3-aminopropyl ammonium hydrochloride (2) have been obtained by the reactions of 1,1-dimethyl-1,3-propylenediamine with ethyl chloroacetate and chloroacetic acid, respectively. The products have been characterized by FTIR, Raman and NMR spectroscopy. B3LYP calculations have also been carried out. The screening constants for 13 C- and 1 H- atoms have been calculated by the GIAO/B3LYP/6-31G(d,p) approach and analyzed. The FTIR and NMR spectra of the investigated compounds 1 and 2 are in excellent agreement with the structures optimized by Density Functional Theory (DFT) calculations

546 THE EFFECT OF OVARIECTOMY ON THE PROTEIN AND NUCLEIC ACID CONTENT OF RAT MAMMARY TUMOURS, INDUCED BY

MAMMARY tumours can be induced in rats by intragastric or intravenous administration of 9,10-dimethyl-1,2-benzanthracene (Huggins, Grand, and Brillantes, 1959). The growth of the majority of these tumours depends on the amounts of circulating oestrogen (Huggins, Briziarelli, and Sutton, 1959; Dao, 1962). Ovariectomy causes regression of the tumours but growth may be restored by additive hormone therapy. A change in the size of the tumours after ovariectomy is not usually detectable for three or four days, but it is probable that many biochemical changes occur before this in preparation for the regression process. At present nothing is known about the mechanism of this process. The purpose of this work is to investigate possible changes in nucleic acid and protein levels, within days of ovariectomy. MATERIALS AND METHODS The methods of induction, detection, and assessment of the biological activity of mammary tumours have been previously described (Stevens, Stevens, and Currie, 1965). Ovariectomy and subsequent tumour biopsies were carried ou

R[F 2>2 (F 2)] = 0.083

diffractometer Absorption correction: scan (North et al., 1968) T min = 0.981, T max = 0.995 2069 measured reflections Refinemen

Refinement

wR(F 2) = 0.11

Prepared By John Diggory Revision Date: 22 OCT 10 Section II- HAZARD IDENTIFICATION

Classification This Ultralife battery product meets the definition of an article. Under th

Developmental Toxicity in the Rat After Ingestion or Gavage of

The structural development of fetuses was altered when Dipterex was administered by diet to pregnant rats from days 6 through 15 of gestation. Major external and skeletal alterations occurred after consumption of 432 or 519 mg/kg body weight per day, only minor skeletal changes occurred in the 375 mg/kg dose group and the incidence of alterations in the 145 mg/kg dose group was not significantly different from that in the pair-fed controls. The malformations seen at the two highest doses did not result directly from the associated decrease in food consumed. Dipterex was not shown to have teratogenic potential when given for the same time span, once daily by gavage, even at levels that produced maternal lethality. Imidan was not teratogenic when similarly given, either by diet at concentrations that resulted in a 45 % reduction in food consumption, or by gavage at dose levels that resulted in some maternal lethality. Data collected from pair-fed control females revealed that limitation of food consumption to 13-15 g/rat per day from days 6 through 15 of gestation did not result in increased fetal mortality or stunting. However, fetal weight was reduced slightly, and the incidence of minor skeletal changes was approximately three to four times that among fetuses of control dams that were not pair-fed

Kinetics of Appearance of Differentiation-associated Characteristics

The process of drug-induced cell differentiation was studied by comparing the effects 12-O-tetradecanoylphorbol-IS-acetate (TPA), dimethyl sulfoxide (DMSO), and 1-yS-D-arabinofuranosylcytosine (ara-C) exert on the in vitro growth and viability of a human myeloblastic leukemia cell line, ML-1, and by monitoring the kinetics with which these agents induce the appearance of various differentiation-associated characteris tics. An increase in Fc surface receptors became detectable within 6 hr after exposure of the cells to 5 x 10~'Â ° M TPA or 1.6 % DMSO, and this marker was found present in 25 and 40%, respectively, of the viable cells incubated for 24 hr in the presence of these agents. This level remained essentially con stant during the additional 6-day incubation period. After trea