Stimulatory effect of 17 beta-estradiol on osteogenic differentiation potential of rat adipose tissue-derived stem cells

WOS: 000291300600008PubMed ID: 21613672Adipose tissue-derived stem cells (ADSCs) are considered as a potential cell source for regenerative medicine and tissue engineering. Although ADSCs have greater proliferation capacity than bone marrow stem cells (BMSCs), lower differentiation ability of these cells limits their utility in experimental and clinical studies. The purpose of this study was to investigate whether 17 beta-estradiol (E(2)) has a stimulatory effect on osteogenic differentiation potential of ADSCs in vitro. ADSCs were isolated from visceral adipose tissues of rats and treated with different concentrations of E(2) in osteogenic medium (OM) for 21 days. The differences in osteogenic differentiation potential of the cultures were assessed by von Kossa staining, measurement of alkaline phosphatase (ALP) activity and calcium levels. ADSCs cultured in OM supplemented with E(2) showed greater bone-like nodule formation and mineral deposition in comparing with the cells grown in OM. In addition, ALP activity and calcium levels also were significantly higher in the cultures exposed to E(2) than the cells treated only with OM (p < 0.005, n = 5). Our results suggest that E(2) may stimulate the osteogenic differentiation of ADSCs and therefore, can be used as an inducing agent to improve the efficiency of these cells in in vitro and in vivo studies

The investigation of protective effects of glucagon-like peptide-1 (GLP-1) analogue exenatide against glucose and fructose-induced neurotoxicity

###EgeUn###Diabetes mellitus (DM) is one of the most common metabolic disorders characterized by hyperglycemia due to insufficiency of insulin and/or insulin resistance. Clinical studies have revealed a higher risk of neurodegenerative disorders such as Alzheimer's disease or Parkinson's disease in diabetic patients. Recently, glucagon-like peptide-1 (GLP-1) is an attractive potential treatment modality for various neurodegenerative diseases. In our study, we aimed to investigate whether exenatide, a GLP-1 analogue, has neuroprotective effects against glucose and fructose-induced toxicity in human SH-SY5Y neuroblastoma cell line. Neurotoxicity was induced by incubating SH-SY5Y cells with different doses (25-100 mM) of glucose and fructose for 24, 48 and 72 hours. Following determination of the significant toxic doses of glucose and fructose, the cells were treated with various doses of exenatide (10-250 nM) in the presence or absence of glucose and fructose. Neurotoxicity was evaluated by MTT assay and Hoechst 33258 staining. Caspase-3 activity and the levels of advanced glycation end products (AGEs) were determined in the cytosolic fractions of treated cells. Our results demonstrated that both glucose and fructose treatments decreased cell viability in neuronal cells dose and time-dependently. Glucose and fructose-treated groups showed increased numbers of apoptotic cells, caspase-3 activity and AGEs levels. Treatment of the cells with exenatide significantly prevented cell death. The most prominent effect was observed at 100 nM exenatide-treated cultures. Our results suggest that high doses of glucose and fructose may lead to neurotoxicity, and exenatide may have protective effects against neuronal damage through its anti-apoptotic feature.TUBITAK (The Scientific and Technological Research Council of TURKEY)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [115S136]; Ege University Research FoundationEge University [2016-TIP-010]This study was supported by grants from TUBITAK (The Scientific and Technological Research Council of TURKEY) (No. 115S136) and Ege University Research Foundation (No. 2016-TIP-010)

NEPHROPROTECTIVE EFFECT OF ADENOSINE IN STREPTOZOTOCIN INDUCED DIABETIC RATS

53rd ERA-EDTA Congress -- MAY 21-24, 2016 -- Vienna, AUSTRIAWOS: 000376653801327European Renal Assoc, European Dialysis & Transplant Asso

NEPHROPROTECTIVE EFFECT OF ADENOSINE IN STREPTOZOTOCIN INDUCED DIABETIC RATS

53rd ERA-EDTA Congress -- MAY 21-24, 2016 -- Vienna, AUSTRIAWOS: 000376653801327…European Renal Assoc, European Dialysis & Transplant Asso

Granulocyte colony-stimulating factor provides protection against cardiovascular autonomic neuropathy in streptozotocin-induced diabetes in rats

WOS: 000349959600012PubMed ID: 25638453Aims: Cardiovascular autonomic neuropathy (CAN) is a relatively common and detrimental complication of diabetes mellitus (DM). Dysregulation of neuropeptides, such as calcitonin gene-related peptide (CGRP) and vasoactive intestinal peptide (VIP), are thought to play significant roles in diabetes-related cardiovascular disease. Accumulating evidence indicates the neuroprotective effects of granulocyte-colony stimulating factor (G-CSF) in different neurological disorders. The purpose of the study is to investigate the role of CGRP and VIP and possible effects of G-CSF on CAN in type I DM model in rats. Methods: Diabetes was induced by intraperitoneal injection of streptozotocin (STZ) for 14 rats. Seven rats served as controls and 6 rats were administered G-CSF alone. DM group was randomly divided into 2 groups and received either 1 mL/kg saline (DM + saline group) or 100 mu g/kg/day G-CSF (DM + G-CSF group) for 4 weeks. Following electrocardiography (ECG), GCRP and VIP levels were measured in plasma samples. Results: Diabetes promoted a significant prolongation in the corrected QT interval (cQT) (P < 0.001) whereas G-CSF administration significantly shortened cQT interval (P < 0.05). Plasma VIP and CGRP levels of saline treated DM group were significantly lower than those of control group (P < 0.05). G-CSF treatment significantly prevented the reduction in plasma VIP and CGRP levels (P < 0.01 and P < 0.05, respectively). Also, correlation analysis showed a significant negative correlation between the cQT and neuropeptide levels. Conclusions: This study suggests that G-CSF can be effective in CAN by means of neuro-protection, and plasma VIP and CGRP levels can be used for the assessment of autonomic and sensory functions in diabetes. (C) 2015 Elsevier Ireland Ltd. All rights reserved

Student Nurses' Assessment of Medical Errors

Purpose: The aim of this study was to evaluate the quality of nursing students' assessment of cases of medical error. Method: This descriptive cross-sectional study was conducted with 145 nursing students in Izmir, Turkey. The epidemiology of the medical errors that the students witnessed during clinical practice was examined. Then, the success of the students in using the Text-Based Medical Error cases tool developed by the researchers was examined. Results: Of the students, 24.1% had witnessed medical errors during clinical practice. The percentage of students successfully analyzing cases of medical errors related to patient identification, falling, medication administration, blood transfusions, health-care-associated infections, and pressure ulcers were 51.72%, 7.59%, 17.24%, 8.28%, 45.52%, and 56.55%, respectively. Conclusion: The students' ability to evaluate cases of medical error needs improvement