The German 79th Reserve Infantry Division in the Battle of Vimy Ridge, April 1917

Canadian narratives of the Great War rarely give a very clear picture of the enemy. They may mention German regiments, brigades, divisions or corps that opposed Canadian troops on this or that front, but detailed citations from German sources are often missing. There are a number of reasons for this, most notably the scarcity of translated primary documents. A number of comprehensive regimental histories were published in Germany during the interwar years, but these are often difficult to obtain and tend to be printed in old German. Consequently, we were glad to receive this translation of Generalleutnant Alfred Dieterich’s report on his brigade’s defensive operations on Vimy Ridge during March-April 1917. Dieterich originally published the account in Germany after the war, and to our knowledge, this is the first time it has appeared in English. The translation was completed by Christopher and Ute Wilde Linnell

Proteogenomics of Pristionchus pacificus reveals distinct proteome structure of nematode models

Pristionchus pacificus is a nematode model organism whose genome has recently been sequenced. To refine the genome annotation we performed transcriptome and proteome analysis and gathered comprehensive experimental information on gene expression. Transcriptome analysis on a 454 Life Sciences (Roche) FLX platform generated >700,000 expressed sequence tags (ESTs) from two normalized EST libraries, whereas proteome analysis on an LTQ-Orbitrap mass spectrometer detected >27,000 nonredundant peptide sequences from more than 4000 proteins at sub-parts-per-million (ppm) mass accuracy and a false discovery rate of <1%. Retraining of the SNAP gene prediction algorithm using the gene expression data led to a decrease in the number of previously predicted protein-coding genes from 29,000 to 24,000 and refinement of numerous gene models. The P. pacificus proteome contains a high proportion of small proteins with no known homologs in other species (“pioneer” proteins). Some of these proteins appear to be products of highly homologous genes, pointing to their common origin. We show that >50% of all pioneer genes are transcribed under standard culture conditions and that pioneer proteins significantly contribute to a unimodal distribution of predicted protein sizes in P. pacificus, which has an unusually low median size of 240 amino acids (26.8 kDa). In contrast, the predicted proteome of Caenorhabditis elegans follows a distinct bimodal protein size distribution, with significant functional differences between small and large protein populations. Combined, these results provide the first catalog of the expressed genome of P. pacificus, refinement of its genome annotation, and the first comparison of related nematode models at the proteome level

The SRF Target Gene Fhl2 Antagonizes RhoA/MAL-Dependent Activation of SRF

RhoA signaling regulates the activity of the transcription factor SRF (serum response factor) during muscle differentiation. How RhoA signaling is integrated at SRF target promoters to achieve muscle-lineage-specific expression is largely unknown. Using large-scale expression profiling combined with bioinformatic and biochemical approaches, we identified several SRF target genes, including Fhl2, encoding a transcriptional cofactor that is highly expressed in the heart. SRF binds the Fhl2 promoter in vivo and regulates Fhl2 expression in response to RhoA activation. FHL2 protein and SRF interact physically, and FHL2 binds the promoters of SRF-responsive smooth muscle (SM) genes, but not the promoters of immediate-early genes (IEGs), in response to RhoA. FHL2 antagonizes induction of SM genes, but not IEGs or cardiac genes, by competing with the coactivator MAL/MRTF-A for SRF binding. Our findings identify an autoregulatory mechanism to selectively regulate subsets of RhoA-activated SRF target genes.National Institutes of Health (U.S.) (Grant GM58801