VEGF expression by mesenchymal stem cells contributes to angiogenesis in pancreatic carcinoma

Little is known about the factors that enable the mobilisation of human mesenchymal stem cells (MSC) from the bone marrow into the blood stream and their recruitment to and retention in the tumour. We found specific migration of MSC towards growth factors present in pancreatic tumours, such as PDGF, EGF, VEGF and specific inhibitors Glivec, Erbitux and Avastin interfered with migration. Within a few hours, MSC migrated into spheroids consisting of pancreatic cancer cells, fibroblasts and endothelial cells as measured by time-lapse microscopy. Supernatant from subconfluent MSC increased sprouting of HUVEC due to VEGF production by MSC itself as demonstrated by RT-PCR and ELISA. Only few MSCs were differentiated into endothelial cells in vitro, whereas in vivo differentiation was not observed. Lentiviral GFP-marked MSCs, injected in nude mice xenografted with orthotopic pancreatic tumours, preferentially migrated into the tumours as observed by FACS analysis of green fluorescent cells. By immunofluorescence and intravital microscopic studies, we found the interaction of MSC with the endothelium of blood vessels. Mesenchymal stem cells supported tumour angiogenesis in vivo, that is CD31+ vessel density was increased after the transfer of MSC compared with siVEGF-MSC. Our data demonstrate the migration of MSC toward tumour vessels and suggest a supportive role in angiogenesis

Using computer simulation to aid the research of drilling processes

Drilling wells is one of the primary methods used for mineral exploration. Scientific studies have aimed at improving the technical and economic aspects of drilling because of the current competitive economic conditions. Note that the primary topic of these studies has been developing new effective rock-cutting tools. To design a new rock-cutting tool, a thorough, reliable, and accurate study of the processes that occur during drilling is necessary. During drilling, mechanical, hydraulic, thermal, and chemical phenomena, which are interdependent and affect the performance of a drilling tool, simultaneously occur; therefore, a systematic, integrated approach is required for studying drilling processes. Field-based and laboratory experiments are quite tedious to perform and require high material costs, and it is often not possible to separately evaluate small elements of the drilling model. Therefore, computer simulation is an important research tool that enables accurate and reliable visualization of even small parts of the model. The aim. The aim of this study is to examine the potential for computer simulation of the processes that occur during drilling. Objective. In this study, we evaluated the simulation features of various software products, such as KOMPAS-3D, ANSYS, Delphi, and LabVIEW, for their utility in studying the processes that occur during drilling. The possibility of computer simulation for studying drilling processes, including its advantages and disadvantages, are demonstrated. The results are obtained from a model that simulates a rock cutting tool. The main uses of the rock cutting tool are outlined, and the drilling simulation development is planned. Choice of research method. The study of the capabilities of existing modern software products, for use in drilling process research, is carried out by an analytical review method

Schleswig-Holsteinisches Abfall-Forum Kongressbericht

Die Situation der Schleswig-Holsteinischen Abfallwirtschaft wird im Zeichen des neuen Abfallgesetzes, sowie des darin enthaltenen Gesetzes zur Umweltvertraeglichkeitspruefung dargestellt. Die Diskussionsergebnisse liefern Orientierungen weit ueber die Landesgrenzen hinaus. (BBR)The situation of waste management in Schleswig-Holstein is reviewed in consideration of the new Waste Management Act and the Act on Environmental Acceptability Statements comprised therein. The results of the discussions go far beyond the Laender level. (BBR)SIGLEAvailable from TIB Hannover: RO 9545(5) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Sonnenstrahlung Bestimmung der flaechenhaften Verteilung und der spektralen Bestrahlungsstaerke

SIGLEAvailable from TIB Hannover / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman

Messtechnische Untersuchungen am Windpark Westkueste

The wind park Westkueste consisting of 32 wind turbines and a maximum power output of 1.33 MW is the first large wind park in Germany. As a demonstration and research plant a measuring system has been developed in order to investigate aerodynamic influences of the turbines, operation and electrical parameters and question of the technical and real reliability. The broshure gives a short presentation on the measuring system. (BWI)Der Windpark Westkueste ist mit 32 Windkraftanlagen und einer maximalen Gesamtleistung von 1,33 MW der erste grosse Windpark in Deutschland. Als Demonstrations- und Versuchsvorhaben wurde die Anlage messtechnisch ueberwacht, um Fragen der gegenseitigen aerodynamischen und elektrischen Beeinflussung, der Betriebsfuehrung sowie der technischen und tatsaechlichen Verfuegbarkeit zu untersuchen. Im Rahmen der Broschuere wird das Messsystem kurz vorgestellt. (BWI)Available from TIB Hannover / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman

Simulation thermischer Solaranlagen

The brochure presents methods of calculation for solar systems. The simulation program comprises the following data: Meteorological data, insolation data, system components, thermal load, auxiliary heating energy required. (orig.)Es wird berichtet ueber Berechungsverfahren fuer thermische Solaranlagen. Bestandteile des Simulationsprogramms: Wetterdaten, Einstrahlungsdaten, Systemkomponenten, Waermelast, Zusatzheizenergie. (HW)SIGLEAvailable from TIB Hannover: ZO 7282(1994,9) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman